Overexpression of a pepper basic pathogenesis-related protein 1 gene in tobacco plants enhances resistance to heavy metal and pathogen stresses

Sujon Sarowar, Jin Kim Young, Nam Kim Eui, Ki Deok Kim, Kook Hwang Byung, Rafiul Islam, Jeong Sheop Shin

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

A pepper gene, CABPR1, which encodes basic pathogenesis-related protein 1, has been reported to be strongly induced after ethephon treatment, wounding, and tobacco mosaic virus infection. The potential role of CABPR1 in tolerance of biotic or abiotic stresses was examined in transgenic Nicotiana tabacum cv. xanthi plants. Overexpression of CABPR1 in tobacco plants enhanced tolerance not only to heavy metal stresses, but also to the oomycete pathogen Phytophthora nicotianae, and the bacterial pathogens Ralstonia solanacearum and Pseudomonas syringae pv. tabaci. RT-PCR revealed that the CABPR1 transgene increased expression of the PR-Q and glutathione S-transferase genes, but decreased expression of the PR-1a and thaumatin genes. Moreover, these transgenic lines exhibited significant decreases in total peroxidase activity and transcription level, suggesting that overexpression of CABPR1 in tobacco cells altered the balance of redox systems. Redox imbalance in transgenic lines may lead to H 2O 2 accumulation, triggering tolerance to biotic and abiotic stresses.

Original languageEnglish
Pages (from-to)216-224
Number of pages9
JournalPlant Cell Reports
Volume24
Issue number4
DOIs
Publication statusPublished - 2005 Jun 1

Fingerprint

pathogenesis-related proteins
pepper
heavy metals
tobacco
genetically modified organisms
biotic stress
abiotic stress
pathogens
Pseudomonas syringae pv. tabaci
Phytophthora nicotianae
Ralstonia solanacearum
Tobacco mosaic virus
genes
Oomycetes
ethephon
glutathione transferase
Nicotiana tabacum
transgenes
peroxidase
transcription (genetics)

Keywords

  • CABPR1-Capsicum annuum basic pathogenesis-related protein 1
  • Heavy metal
  • Phytophthora nicotianae
  • Pseudomonas syringae pv. tabaci
  • Ralstonia solanacearum

ASJC Scopus subject areas

  • Plant Science

Cite this

Overexpression of a pepper basic pathogenesis-related protein 1 gene in tobacco plants enhances resistance to heavy metal and pathogen stresses. / Sarowar, Sujon; Young, Jin Kim; Eui, Nam Kim; Kim, Ki Deok; Byung, Kook Hwang; Islam, Rafiul; Shin, Jeong Sheop.

In: Plant Cell Reports, Vol. 24, No. 4, 01.06.2005, p. 216-224.

Research output: Contribution to journalArticle

@article{7509899a8c6d432c813b16f1e076e5a0,
title = "Overexpression of a pepper basic pathogenesis-related protein 1 gene in tobacco plants enhances resistance to heavy metal and pathogen stresses",
abstract = "A pepper gene, CABPR1, which encodes basic pathogenesis-related protein 1, has been reported to be strongly induced after ethephon treatment, wounding, and tobacco mosaic virus infection. The potential role of CABPR1 in tolerance of biotic or abiotic stresses was examined in transgenic Nicotiana tabacum cv. xanthi plants. Overexpression of CABPR1 in tobacco plants enhanced tolerance not only to heavy metal stresses, but also to the oomycete pathogen Phytophthora nicotianae, and the bacterial pathogens Ralstonia solanacearum and Pseudomonas syringae pv. tabaci. RT-PCR revealed that the CABPR1 transgene increased expression of the PR-Q and glutathione S-transferase genes, but decreased expression of the PR-1a and thaumatin genes. Moreover, these transgenic lines exhibited significant decreases in total peroxidase activity and transcription level, suggesting that overexpression of CABPR1 in tobacco cells altered the balance of redox systems. Redox imbalance in transgenic lines may lead to H 2O 2 accumulation, triggering tolerance to biotic and abiotic stresses.",
keywords = "CABPR1-Capsicum annuum basic pathogenesis-related protein 1, Heavy metal, Phytophthora nicotianae, Pseudomonas syringae pv. tabaci, Ralstonia solanacearum",
author = "Sujon Sarowar and Young, {Jin Kim} and Eui, {Nam Kim} and Kim, {Ki Deok} and Byung, {Kook Hwang} and Rafiul Islam and Shin, {Jeong Sheop}",
year = "2005",
month = "6",
day = "1",
doi = "10.1007/s00299-005-0928-x",
language = "English",
volume = "24",
pages = "216--224",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - Overexpression of a pepper basic pathogenesis-related protein 1 gene in tobacco plants enhances resistance to heavy metal and pathogen stresses

AU - Sarowar, Sujon

AU - Young, Jin Kim

AU - Eui, Nam Kim

AU - Kim, Ki Deok

AU - Byung, Kook Hwang

AU - Islam, Rafiul

AU - Shin, Jeong Sheop

PY - 2005/6/1

Y1 - 2005/6/1

N2 - A pepper gene, CABPR1, which encodes basic pathogenesis-related protein 1, has been reported to be strongly induced after ethephon treatment, wounding, and tobacco mosaic virus infection. The potential role of CABPR1 in tolerance of biotic or abiotic stresses was examined in transgenic Nicotiana tabacum cv. xanthi plants. Overexpression of CABPR1 in tobacco plants enhanced tolerance not only to heavy metal stresses, but also to the oomycete pathogen Phytophthora nicotianae, and the bacterial pathogens Ralstonia solanacearum and Pseudomonas syringae pv. tabaci. RT-PCR revealed that the CABPR1 transgene increased expression of the PR-Q and glutathione S-transferase genes, but decreased expression of the PR-1a and thaumatin genes. Moreover, these transgenic lines exhibited significant decreases in total peroxidase activity and transcription level, suggesting that overexpression of CABPR1 in tobacco cells altered the balance of redox systems. Redox imbalance in transgenic lines may lead to H 2O 2 accumulation, triggering tolerance to biotic and abiotic stresses.

AB - A pepper gene, CABPR1, which encodes basic pathogenesis-related protein 1, has been reported to be strongly induced after ethephon treatment, wounding, and tobacco mosaic virus infection. The potential role of CABPR1 in tolerance of biotic or abiotic stresses was examined in transgenic Nicotiana tabacum cv. xanthi plants. Overexpression of CABPR1 in tobacco plants enhanced tolerance not only to heavy metal stresses, but also to the oomycete pathogen Phytophthora nicotianae, and the bacterial pathogens Ralstonia solanacearum and Pseudomonas syringae pv. tabaci. RT-PCR revealed that the CABPR1 transgene increased expression of the PR-Q and glutathione S-transferase genes, but decreased expression of the PR-1a and thaumatin genes. Moreover, these transgenic lines exhibited significant decreases in total peroxidase activity and transcription level, suggesting that overexpression of CABPR1 in tobacco cells altered the balance of redox systems. Redox imbalance in transgenic lines may lead to H 2O 2 accumulation, triggering tolerance to biotic and abiotic stresses.

KW - CABPR1-Capsicum annuum basic pathogenesis-related protein 1

KW - Heavy metal

KW - Phytophthora nicotianae

KW - Pseudomonas syringae pv. tabaci

KW - Ralstonia solanacearum

UR - http://www.scopus.com/inward/record.url?scp=21444432153&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21444432153&partnerID=8YFLogxK

U2 - 10.1007/s00299-005-0928-x

DO - 10.1007/s00299-005-0928-x

M3 - Article

C2 - 15719238

AN - SCOPUS:21444432153

VL - 24

SP - 216

EP - 224

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 4

ER -