Overexpression of a pepper basic pathogenesis-related protein 1 gene in tobacco plants enhances resistance to heavy metal and pathogen stresses

Sujon Sarowar, Jin Kim Young, Nam Kim Eui, Deok Kim Ki, Kook Hwang Byung, Rafiul Islam, Sheop Shin Jeong

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

A pepper gene, CABPR1, which encodes basic pathogenesis-related protein 1, has been reported to be strongly induced after ethephon treatment, wounding, and tobacco mosaic virus infection. The potential role of CABPR1 in tolerance of biotic or abiotic stresses was examined in transgenic Nicotiana tabacum cv. xanthi plants. Overexpression of CABPR1 in tobacco plants enhanced tolerance not only to heavy metal stresses, but also to the oomycete pathogen Phytophthora nicotianae, and the bacterial pathogens Ralstonia solanacearum and Pseudomonas syringae pv. tabaci. RT-PCR revealed that the CABPR1 transgene increased expression of the PR-Q and glutathione S-transferase genes, but decreased expression of the PR-1a and thaumatin genes. Moreover, these transgenic lines exhibited significant decreases in total peroxidase activity and transcription level, suggesting that overexpression of CABPR1 in tobacco cells altered the balance of redox systems. Redox imbalance in transgenic lines may lead to H 2O2 accumulation, triggering tolerance to biotic and abiotic stresses.

Original languageEnglish
Pages (from-to)216-224
Number of pages9
JournalPlant Cell Reports
Volume24
Issue number4
DOIs
Publication statusPublished - 2005 Jun

Keywords

  • CABPR1-Capsicum annuum basic pathogenesis-related protein 1
  • Heavy metal
  • Phytophthora nicotianae
  • Pseudomonas syringae pv. tabaci
  • Ralstonia solanacearum

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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