TY - JOUR
T1 - Overexpression of a pepper basic pathogenesis-related protein 1 gene in tobacco plants enhances resistance to heavy metal and pathogen stresses
AU - Sarowar, Sujon
AU - Young, Jin Kim
AU - Eui, Nam Kim
AU - Ki, Deok Kim
AU - Byung, Kook Hwang
AU - Islam, Rafiul
AU - Jeong, Sheop Shin
N1 - Funding Information:
Acknowledgements This study was supported by the special grants from the Agricultural R & D Promotion Center, Ministry of Agri- culture and Forestry, and by grants from PDRC (M103KD010057-04K0401-05710) of the 21st Century Frontier Research Program and SIGNET (R11-2003-008-02006) funded by the Ministry of Science and Technology (MOST), Korea.
PY - 2005/6
Y1 - 2005/6
N2 - A pepper gene, CABPR1, which encodes basic pathogenesis-related protein 1, has been reported to be strongly induced after ethephon treatment, wounding, and tobacco mosaic virus infection. The potential role of CABPR1 in tolerance of biotic or abiotic stresses was examined in transgenic Nicotiana tabacum cv. xanthi plants. Overexpression of CABPR1 in tobacco plants enhanced tolerance not only to heavy metal stresses, but also to the oomycete pathogen Phytophthora nicotianae, and the bacterial pathogens Ralstonia solanacearum and Pseudomonas syringae pv. tabaci. RT-PCR revealed that the CABPR1 transgene increased expression of the PR-Q and glutathione S-transferase genes, but decreased expression of the PR-1a and thaumatin genes. Moreover, these transgenic lines exhibited significant decreases in total peroxidase activity and transcription level, suggesting that overexpression of CABPR1 in tobacco cells altered the balance of redox systems. Redox imbalance in transgenic lines may lead to H 2O2 accumulation, triggering tolerance to biotic and abiotic stresses.
AB - A pepper gene, CABPR1, which encodes basic pathogenesis-related protein 1, has been reported to be strongly induced after ethephon treatment, wounding, and tobacco mosaic virus infection. The potential role of CABPR1 in tolerance of biotic or abiotic stresses was examined in transgenic Nicotiana tabacum cv. xanthi plants. Overexpression of CABPR1 in tobacco plants enhanced tolerance not only to heavy metal stresses, but also to the oomycete pathogen Phytophthora nicotianae, and the bacterial pathogens Ralstonia solanacearum and Pseudomonas syringae pv. tabaci. RT-PCR revealed that the CABPR1 transgene increased expression of the PR-Q and glutathione S-transferase genes, but decreased expression of the PR-1a and thaumatin genes. Moreover, these transgenic lines exhibited significant decreases in total peroxidase activity and transcription level, suggesting that overexpression of CABPR1 in tobacco cells altered the balance of redox systems. Redox imbalance in transgenic lines may lead to H 2O2 accumulation, triggering tolerance to biotic and abiotic stresses.
KW - CABPR1-Capsicum annuum basic pathogenesis-related protein 1
KW - Heavy metal
KW - Phytophthora nicotianae
KW - Pseudomonas syringae pv. tabaci
KW - Ralstonia solanacearum
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U2 - 10.1007/s00299-005-0928-x
DO - 10.1007/s00299-005-0928-x
M3 - Article
C2 - 15719238
AN - SCOPUS:21444432153
VL - 24
SP - 216
EP - 224
JO - Plant Cell Reports
JF - Plant Cell Reports
SN - 0721-7714
IS - 4
ER -