Abstract
A pepper gene, CABPR1, which encodes basic pathogenesis-related protein 1, has been reported to be strongly induced after ethephon treatment, wounding, and tobacco mosaic virus infection. The potential role of CABPR1 in tolerance of biotic or abiotic stresses was examined in transgenic Nicotiana tabacum cv. xanthi plants. Overexpression of CABPR1 in tobacco plants enhanced tolerance not only to heavy metal stresses, but also to the oomycete pathogen Phytophthora nicotianae, and the bacterial pathogens Ralstonia solanacearum and Pseudomonas syringae pv. tabaci. RT-PCR revealed that the CABPR1 transgene increased expression of the PR-Q and glutathione S-transferase genes, but decreased expression of the PR-1a and thaumatin genes. Moreover, these transgenic lines exhibited significant decreases in total peroxidase activity and transcription level, suggesting that overexpression of CABPR1 in tobacco cells altered the balance of redox systems. Redox imbalance in transgenic lines may lead to H 2O 2 accumulation, triggering tolerance to biotic and abiotic stresses.
| Original language | English |
|---|---|
| Pages (from-to) | 216-224 |
| Number of pages | 9 |
| Journal | Plant Cell Reports |
| Volume | 24 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2005 Jun 1 |
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Keywords
- CABPR1-Capsicum annuum basic pathogenesis-related protein 1
- Heavy metal
- Phytophthora nicotianae
- Pseudomonas syringae pv. tabaci
- Ralstonia solanacearum
ASJC Scopus subject areas
- Plant Science
Cite this
Overexpression of a pepper basic pathogenesis-related protein 1 gene in tobacco plants enhances resistance to heavy metal and pathogen stresses. / Sarowar, Sujon; Young, Jin Kim; Eui, Nam Kim; Kim, Ki Deok; Byung, Kook Hwang; Islam, Rafiul; Shin, Jeong Sheop.
In: Plant Cell Reports, Vol. 24, No. 4, 01.06.2005, p. 216-224.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Overexpression of a pepper basic pathogenesis-related protein 1 gene in tobacco plants enhances resistance to heavy metal and pathogen stresses
AU - Sarowar, Sujon
AU - Young, Jin Kim
AU - Eui, Nam Kim
AU - Kim, Ki Deok
AU - Byung, Kook Hwang
AU - Islam, Rafiul
AU - Shin, Jeong Sheop
PY - 2005/6/1
Y1 - 2005/6/1
N2 - A pepper gene, CABPR1, which encodes basic pathogenesis-related protein 1, has been reported to be strongly induced after ethephon treatment, wounding, and tobacco mosaic virus infection. The potential role of CABPR1 in tolerance of biotic or abiotic stresses was examined in transgenic Nicotiana tabacum cv. xanthi plants. Overexpression of CABPR1 in tobacco plants enhanced tolerance not only to heavy metal stresses, but also to the oomycete pathogen Phytophthora nicotianae, and the bacterial pathogens Ralstonia solanacearum and Pseudomonas syringae pv. tabaci. RT-PCR revealed that the CABPR1 transgene increased expression of the PR-Q and glutathione S-transferase genes, but decreased expression of the PR-1a and thaumatin genes. Moreover, these transgenic lines exhibited significant decreases in total peroxidase activity and transcription level, suggesting that overexpression of CABPR1 in tobacco cells altered the balance of redox systems. Redox imbalance in transgenic lines may lead to H 2O 2 accumulation, triggering tolerance to biotic and abiotic stresses.
AB - A pepper gene, CABPR1, which encodes basic pathogenesis-related protein 1, has been reported to be strongly induced after ethephon treatment, wounding, and tobacco mosaic virus infection. The potential role of CABPR1 in tolerance of biotic or abiotic stresses was examined in transgenic Nicotiana tabacum cv. xanthi plants. Overexpression of CABPR1 in tobacco plants enhanced tolerance not only to heavy metal stresses, but also to the oomycete pathogen Phytophthora nicotianae, and the bacterial pathogens Ralstonia solanacearum and Pseudomonas syringae pv. tabaci. RT-PCR revealed that the CABPR1 transgene increased expression of the PR-Q and glutathione S-transferase genes, but decreased expression of the PR-1a and thaumatin genes. Moreover, these transgenic lines exhibited significant decreases in total peroxidase activity and transcription level, suggesting that overexpression of CABPR1 in tobacco cells altered the balance of redox systems. Redox imbalance in transgenic lines may lead to H 2O 2 accumulation, triggering tolerance to biotic and abiotic stresses.
KW - CABPR1-Capsicum annuum basic pathogenesis-related protein 1
KW - Heavy metal
KW - Phytophthora nicotianae
KW - Pseudomonas syringae pv. tabaci
KW - Ralstonia solanacearum
UR - http://www.scopus.com/inward/record.url?scp=21444432153&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21444432153&partnerID=8YFLogxK
U2 - 10.1007/s00299-005-0928-x
DO - 10.1007/s00299-005-0928-x
M3 - Article
VL - 24
SP - 216
EP - 224
JO - Plant Cell Reports
JF - Plant Cell Reports
SN - 0721-7714
IS - 4
ER -