Overexpression of cyclin DI is rare in human prostate carcinoma

Laura M. Gumbiner, Paul H. Gumerlock, Philip C. Mack, Sung-Gil Chi, Ralph W. DeVere White, James L. Mohler, Thomas G. Pretlow, James V. Tricoli

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

BACKGROUND. Overexpression of cyclin D1 has been documented in a number of human cancers. Increased expression of cyclin D1 can contribute to cellular transformation and abnormal proliferation. METHODS. Quantitative RT- PCR and/or Western blot analysis were used to determine the level of cyclin D1 expression in 96 human prostate tumors, 15 benign prostate hyperplasias, 4 prostate cancer cell lines, and 3 xenografts. RESULTS. Our results demonstrate that 4.2% of the prostate tumors examined overexpressed cyclin D1 transcripts. In the cell lines, expression was normal, with the exception that reduced levels of cyclin D1 transcript and protein were observed in the DU145 cell line, as expected from cells with mutant RB. Normal levels of cyclin D1 were found in all xenograft tumors and BPH specimens examined. CONCLUSIONS. These data show that overexpression of cyclin D1 occurs rarely in human prostate tumors. However, when overexpression of cyclin D1 does occur, it may identify a subset of tumors with a different molecular biology.

Original languageEnglish
Pages (from-to)40-45
Number of pages6
JournalProstate
Volume38
Issue number1
DOIs
Publication statusPublished - 1999 Jan 8
Externally publishedYes

Fingerprint

Cyclins
Cyclin D1
Prostate
Carcinoma
Neoplasms
Heterografts
Cell Line
Hyperplasia
Molecular Biology
Prostatic Neoplasms
Western Blotting
Polymerase Chain Reaction

Keywords

  • Cyclin D1
  • Expression
  • Prostate cancer
  • Retinoblastoma
  • RT-PCR

ASJC Scopus subject areas

  • Urology

Cite this

Gumbiner, L. M., Gumerlock, P. H., Mack, P. C., Chi, S-G., DeVere White, R. W., Mohler, J. L., ... Tricoli, J. V. (1999). Overexpression of cyclin DI is rare in human prostate carcinoma. Prostate, 38(1), 40-45. https://doi.org/10.1002/(SICI)1097-0045(19990101)38:1<40::AID-PROS5>3.0.CO;2-I

Overexpression of cyclin DI is rare in human prostate carcinoma. / Gumbiner, Laura M.; Gumerlock, Paul H.; Mack, Philip C.; Chi, Sung-Gil; DeVere White, Ralph W.; Mohler, James L.; Pretlow, Thomas G.; Tricoli, James V.

In: Prostate, Vol. 38, No. 1, 08.01.1999, p. 40-45.

Research output: Contribution to journalArticle

Gumbiner, LM, Gumerlock, PH, Mack, PC, Chi, S-G, DeVere White, RW, Mohler, JL, Pretlow, TG & Tricoli, JV 1999, 'Overexpression of cyclin DI is rare in human prostate carcinoma', Prostate, vol. 38, no. 1, pp. 40-45. https://doi.org/10.1002/(SICI)1097-0045(19990101)38:1<40::AID-PROS5>3.0.CO;2-I
Gumbiner, Laura M. ; Gumerlock, Paul H. ; Mack, Philip C. ; Chi, Sung-Gil ; DeVere White, Ralph W. ; Mohler, James L. ; Pretlow, Thomas G. ; Tricoli, James V. / Overexpression of cyclin DI is rare in human prostate carcinoma. In: Prostate. 1999 ; Vol. 38, No. 1. pp. 40-45.
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