Abstract
In our previous work, we developed an auxotrophic mutant of Saccharomyces cerevisiae sake kyokai No. 6 strain for the selection of recombinant DNA. Sake yeast can accumulate high amount of S-adenosylmethionine (SAM) that is effective for treating depression, Alzheimer's disease and so on. In this work, we further introduced SAM2 gene and ERC1, a frame-shift mutant of YHR032w, to maximize the production of S-adenosylmethionine in S. cerevisiae sake K6. The recombinant strain accumulated SAM about half of its dry cell weight.
| Original language | English |
|---|---|
| Pages (from-to) | 587-589 |
| Number of pages | 3 |
| Journal | Korean Journal of Chemical Engineering |
| Volume | 27 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2010 Mar |
Keywords
- Metabolic Engineering
- S-adenosyl-L-methionine
- Saccharomyces cerevisiae sake
ASJC Scopus subject areas
- Chemistry(all)
- Chemical Engineering(all)
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Lee, S. W., Park, B. S., Choi, E. S., & Oh, M. K. (2010). Overexpression of ethionine resistance gene for maximized production of S-adenosylmethionine in Saccharomyces cerevisiae sake kyokai No. 6. Korean Journal of Chemical Engineering, 27(2), 587-589. https://doi.org/10.2478/s11814-010-0100-3