TY - JOUR
T1 - Overexpression of ethionine resistance gene for maximized production of S-adenosylmethionine in Saccharomyces cerevisiae sake kyokai No. 6
AU - Lee, Sang Woo
AU - Park, Bu Soo
AU - Choi, Eun Sil
AU - Oh, Min Kyu
N1 - Funding Information:
This work was supported by the Korea Research Foundation Grant funded by the Korean Government (KRF-2008-331-D00133) and Yang Young Foundation.
PY - 2010/3
Y1 - 2010/3
N2 - In our previous work, we developed an auxotrophic mutant of Saccharomyces cerevisiae sake kyokai No. 6 strain for the selection of recombinant DNA. Sake yeast can accumulate high amount of S-adenosylmethionine (SAM) that is effective for treating depression, Alzheimer's disease and so on. In this work, we further introduced SAM2 gene and ERC1, a frame-shift mutant of YHR032w, to maximize the production of S-adenosylmethionine in S. cerevisiae sake K6. The recombinant strain accumulated SAM about half of its dry cell weight.
AB - In our previous work, we developed an auxotrophic mutant of Saccharomyces cerevisiae sake kyokai No. 6 strain for the selection of recombinant DNA. Sake yeast can accumulate high amount of S-adenosylmethionine (SAM) that is effective for treating depression, Alzheimer's disease and so on. In this work, we further introduced SAM2 gene and ERC1, a frame-shift mutant of YHR032w, to maximize the production of S-adenosylmethionine in S. cerevisiae sake K6. The recombinant strain accumulated SAM about half of its dry cell weight.
KW - Metabolic Engineering
KW - S-adenosyl-L-methionine
KW - Saccharomyces cerevisiae sake
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U2 - 10.2478/s11814-010-0100-3
DO - 10.2478/s11814-010-0100-3
M3 - Article
AN - SCOPUS:77951640334
VL - 27
SP - 587
EP - 589
JO - Korean Journal of Chemical Engineering
JF - Korean Journal of Chemical Engineering
SN - 0256-1115
IS - 2
ER -