Overexpression of ethionine resistance gene for maximized production of S-adenosylmethionine in Saccharomyces cerevisiae sake kyokai No. 6

Sang Woo Lee; Bu Soo Park; Eun Sil Choi; Min Kyu Oh

doi:10.2478/s11814-010-0100-3

Overexpression of ethionine resistance gene for maximized production of S-adenosylmethionine in Saccharomyces cerevisiae sake kyokai No. 6

Sang Woo Lee, Bu Soo Park, Eun Sil Choi, Min Kyu Oh

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

In our previous work, we developed an auxotrophic mutant of Saccharomyces cerevisiae sake kyokai No. 6 strain for the selection of recombinant DNA. Sake yeast can accumulate high amount of S-adenosylmethionine (SAM) that is effective for treating depression, Alzheimer's disease and so on. In this work, we further introduced SAM2 gene and ERC1, a frame-shift mutant of YHR032w, to maximize the production of S-adenosylmethionine in S. cerevisiae sake K6. The recombinant strain accumulated SAM about half of its dry cell weight.

Original language	English
Pages (from-to)	587-589
Number of pages	3
Journal	Korean Journal of Chemical Engineering
Volume	27
Issue number	2
DOIs	https://doi.org/10.2478/s11814-010-0100-3
Publication status	Published - 2010 Mar

Keywords

Metabolic Engineering
S-adenosyl-L-methionine
Saccharomyces cerevisiae sake

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

Access to Document

10.2478/s11814-010-0100-3

Fingerprint Dive into the research topics of 'Overexpression of ethionine resistance gene for maximized production of S-adenosylmethionine in Saccharomyces cerevisiae sake kyokai No. 6'. Together they form a unique fingerprint.

Cite this

@article{52b5703a570d420eb837c4be474d5c4d,

title = "Overexpression of ethionine resistance gene for maximized production of S-adenosylmethionine in Saccharomyces cerevisiae sake kyokai No. 6",

abstract = "In our previous work, we developed an auxotrophic mutant of Saccharomyces cerevisiae sake kyokai No. 6 strain for the selection of recombinant DNA. Sake yeast can accumulate high amount of S-adenosylmethionine (SAM) that is effective for treating depression, Alzheimer's disease and so on. In this work, we further introduced SAM2 gene and ERC1, a frame-shift mutant of YHR032w, to maximize the production of S-adenosylmethionine in S. cerevisiae sake K6. The recombinant strain accumulated SAM about half of its dry cell weight.",

keywords = "Metabolic Engineering, S-adenosyl-L-methionine, Saccharomyces cerevisiae sake",

author = "Lee, {Sang Woo} and Park, {Bu Soo} and Choi, {Eun Sil} and Oh, {Min Kyu}",

year = "2010",

month = mar,

doi = "10.2478/s11814-010-0100-3",

language = "English",

volume = "27",

pages = "587--589",

journal = "Korean Journal of Chemical Engineering",

issn = "0256-1115",

publisher = "Springer New York",

number = "2",

}

TY - JOUR

T1 - Overexpression of ethionine resistance gene for maximized production of S-adenosylmethionine in Saccharomyces cerevisiae sake kyokai No. 6

AU - Lee, Sang Woo

AU - Park, Bu Soo

AU - Choi, Eun Sil

AU - Oh, Min Kyu

PY - 2010/3

Y1 - 2010/3

N2 - In our previous work, we developed an auxotrophic mutant of Saccharomyces cerevisiae sake kyokai No. 6 strain for the selection of recombinant DNA. Sake yeast can accumulate high amount of S-adenosylmethionine (SAM) that is effective for treating depression, Alzheimer's disease and so on. In this work, we further introduced SAM2 gene and ERC1, a frame-shift mutant of YHR032w, to maximize the production of S-adenosylmethionine in S. cerevisiae sake K6. The recombinant strain accumulated SAM about half of its dry cell weight.

AB - In our previous work, we developed an auxotrophic mutant of Saccharomyces cerevisiae sake kyokai No. 6 strain for the selection of recombinant DNA. Sake yeast can accumulate high amount of S-adenosylmethionine (SAM) that is effective for treating depression, Alzheimer's disease and so on. In this work, we further introduced SAM2 gene and ERC1, a frame-shift mutant of YHR032w, to maximize the production of S-adenosylmethionine in S. cerevisiae sake K6. The recombinant strain accumulated SAM about half of its dry cell weight.

KW - Metabolic Engineering

KW - S-adenosyl-L-methionine

KW - Saccharomyces cerevisiae sake

UR - http://www.scopus.com/inward/record.url?scp=77951640334&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951640334&partnerID=8YFLogxK

U2 - 10.2478/s11814-010-0100-3

DO - 10.2478/s11814-010-0100-3

M3 - Article

AN - SCOPUS:77951640334

VL - 27

SP - 587

EP - 589

JO - Korean Journal of Chemical Engineering

JF - Korean Journal of Chemical Engineering

SN - 0256-1115

IS - 2

ER -