Overexpression of ethionine resistance gene for maximized production of S-adenosylmethionine in Saccharomyces cerevisiae sake kyokai No. 6

Sang Woo Lee, Bu Soo Park, Eun Sil Choi, Min Kyu Oh

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In our previous work, we developed an auxotrophic mutant of Saccharomyces cerevisiae sake kyokai No. 6 strain for the selection of recombinant DNA. Sake yeast can accumulate high amount of S-adenosylmethionine (SAM) that is effective for treating depression, Alzheimer's disease and so on. In this work, we further introduced SAM2 gene and ERC1, a frame-shift mutant of YHR032w, to maximize the production of S-adenosylmethionine in S. cerevisiae sake K6. The recombinant strain accumulated SAM about half of its dry cell weight.

Original languageEnglish
Pages (from-to)587-589
Number of pages3
JournalKorean Journal of Chemical Engineering
Volume27
Issue number2
DOIs
Publication statusPublished - 2010 Mar

Keywords

  • Metabolic Engineering
  • S-adenosyl-L-methionine
  • Saccharomyces cerevisiae sake

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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