Overexpression of gene encoding tonoplast intrinsic aquaporin promotes urea transport in Arabidopsis

Sun Hee Kim, Kang Il Kim, Hyun Woo Ju, Hojoung Lee, Suk Whan Hong

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Complementation assay of the urea uptake-defective yeast mutants led to the identification of the Arabidopsis AtTIP4;1 gene encoding the aquaporin. However, its physiological functions still remain elusive. In the present study, histochemical and genetic analyses were performed to understand the physiological roles of AtTIP4;1 in urea uptake. The AtTIP4;1 product was detectible in the roots, but not in the leaves, the stem, and the flower. Its promoter allowed the expression of the β-glucuronidase reporter gene in the roots and the apical meristem in Arabidopsis. The AtTIP4;1 products were induced under nitrogen-deficient conditions. To investigate the role of the tonoplast intrinsic protein in urea transport and developments, Arabidopsis with the loss- and the gain-of-function mutations by T-DNA insertion in AtTIP4;1 and 35S promoter-mediated overexpression of AtTIP4;1 were identified, respectively. The transfer DNA insertion and the AtTIP4; 1-overexpressed plants showed normal growth and development under normal or abiotic stress growth conditions. The urea-uptake studies using 14C-labeled urea revealed higher accumulation of urea in the AtTIP4;1-overexpressed plants. These results provide evidence that overexpression of AtTIP4;1 leads to the increase in the urea-uptake rate in plants without detectable defects to the growth and development.

Original languageEnglish
Pages (from-to)102-110
Number of pages9
JournalJournal of Applied Biological Chemistry
Volume51
Issue number3
DOIs
Publication statusPublished - 2008 Jun 30

Fingerprint

Aquaporins
Gene encoding
Urea
DNA
Glucuronidase
Yeast
Assays
Nitrogen
Genes
Proteins
Defects

Keywords

  • Aquaporins
  • T-DNA insertion mutant
  • Tonoplast intrinsic proteins (TIP)
  • Transgenic plants
  • Urea transport

ASJC Scopus subject areas

  • Organic Chemistry
  • Bioengineering

Cite this

Overexpression of gene encoding tonoplast intrinsic aquaporin promotes urea transport in Arabidopsis. / Kim, Sun Hee; Kim, Kang Il; Ju, Hyun Woo; Lee, Hojoung; Hong, Suk Whan.

In: Journal of Applied Biological Chemistry, Vol. 51, No. 3, 30.06.2008, p. 102-110.

Research output: Contribution to journalArticle

Kim, Sun Hee ; Kim, Kang Il ; Ju, Hyun Woo ; Lee, Hojoung ; Hong, Suk Whan. / Overexpression of gene encoding tonoplast intrinsic aquaporin promotes urea transport in Arabidopsis. In: Journal of Applied Biological Chemistry. 2008 ; Vol. 51, No. 3. pp. 102-110.
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