Overexpression of human erythropoietin (EPO) affects plant morphologies: Retarded vegetative growth in tobacco and male sterility in tobacco and Arabidopsis

Ban Yoon Cheon, Hae Jin Kim, Kyung Hee Oh, Sung Chul Bahn, Ji Hoon Ahn, Jang Won Choi, Sung Han Ok, Jung Myung Bae, Jeong Sheop Shin

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Erythropoietin (EPO) is a glycoprotein used for curing human anemia by regulating the differentiation of erythroid progenitors and the production of red blood cells. To examine the expression of recombinant EPO in plants, pPEV-EP21, in which human epo cDNA under the control of the CaMV 35S promoter, was introduced into tobacco and Arabidopsisvia Agrobacterium tumefaciens-mediated transformation. The RNA expression level of epo in the transgenic lines was initially estimated by Northern blot analysis. Two transgenic lines, which exhibited a high expression level of epo mRNA determined by Northern analysis, were chosen for Western blot analysis to examine the production of EPO proteins. Those two lines, EP21-12 and EP21-14, revealed detectable bands on the immunoblot. Interestingly, constitutive expression of the human epo gene affected the morphologies in transgenic plants such that vegetative growth of transgenic tobacco was retarded, and male sterility was induced in transgenic tobacco and Arabidopsis.

Original languageEnglish
Pages (from-to)541-549
Number of pages9
JournalTransgenic Research
Volume13
Issue number6
DOIs
Publication statusPublished - 2004 Dec 1

Fingerprint

erythropoietin
plant morphology
Male Infertility
male fertility
Erythropoietin
Arabidopsis
Tobacco
vegetative growth
tobacco
genetically modified organisms
Growth
Agrobacterium tumefaciens
Genetically Modified Plants
Northern Blotting
curing (nonfood products)
Anemia
Glycoproteins
Complementary DNA
Erythrocytes
Western Blotting

Keywords

  • Arabidopsis thaliana
  • dwarfism
  • erythropoietin (EPO)
  • male sterility
  • Nicotiana tabacum
  • overexpression
  • phenotypic change

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Biotechnology
  • Food Science

Cite this

Overexpression of human erythropoietin (EPO) affects plant morphologies : Retarded vegetative growth in tobacco and male sterility in tobacco and Arabidopsis. / Cheon, Ban Yoon; Kim, Hae Jin; Oh, Kyung Hee; Bahn, Sung Chul; Ahn, Ji Hoon; Choi, Jang Won; Ok, Sung Han; Bae, Jung Myung; Shin, Jeong Sheop.

In: Transgenic Research, Vol. 13, No. 6, 01.12.2004, p. 541-549.

Research output: Contribution to journalArticle

Cheon, Ban Yoon ; Kim, Hae Jin ; Oh, Kyung Hee ; Bahn, Sung Chul ; Ahn, Ji Hoon ; Choi, Jang Won ; Ok, Sung Han ; Bae, Jung Myung ; Shin, Jeong Sheop. / Overexpression of human erythropoietin (EPO) affects plant morphologies : Retarded vegetative growth in tobacco and male sterility in tobacco and Arabidopsis. In: Transgenic Research. 2004 ; Vol. 13, No. 6. pp. 541-549.
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