Overexpression of lipid transfer protein (LTP) genes enhances resistance to plant pathogens and LTP functions in long-distance systemic signaling in tobacco

Sujon Sarowar, Young Jin Kim, Ki Deok Kim, Byung Kook Hwang, Sung Han Ok, Jeong Sheop Shin

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The lipid signal is essential for the activation of plant defense responses, but downstream components of the signaling pathway are still poorly defined. To investigate the biological functions of pepper lipid transfer protein (LTP), we carried out virus-induced gene silencing (VIGS) in pepper, constitutive expression of CALTPs and grafting experiments in the tobacco plant. Suppression of endogenous CALTPI and CALTPII by VIGS, respectively, resulted in enhanced susceptibility to Xanthomonas campestris pv. vescatoria and pepper mosaic mottle virus in pepper. On the other hand, the constitutive expression of CALTPI and CALTPII genes in tobacco plants showed enhanced resistance to oomycete pathogen, Phytophthora nicotianae and bacterial pathogen, Pseudomonas syringae pv. tabaci. Enhanced resistance is found to be associated with the enhanced CALTP transcript levels in the independent transgenic CALTPI or II tobacco lines. Induced resistance responses in grafted scion leaves revealed that LTP plays a role in long-distance systemic signaling in plants.

Original languageEnglish
Pages (from-to)419-427
Number of pages9
JournalPlant Cell Reports
Volume28
Issue number3
DOIs
Publication statusPublished - 2009 Mar 1

Fingerprint

plant pathogens
tobacco
pepper
gene silencing
Pepper mottle virus
genes
Pseudomonas syringae pv. tabaci
Phytophthora nicotianae
Xanthomonas campestris
viruses
leaf protein
pathogens
Oomycetes
induced resistance
scions
grafting (plants)
genetically modified organisms
lipid transfer proteins
lipids

Keywords

  • Disease resistance
  • Lipid transfer protein
  • Phytophthora nicotianae
  • Pseudomonas syringae pv. tabaci
  • Transgenic plant
  • Virus-induced gene silencing

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Overexpression of lipid transfer protein (LTP) genes enhances resistance to plant pathogens and LTP functions in long-distance systemic signaling in tobacco. / Sarowar, Sujon; Kim, Young Jin; Kim, Ki Deok; Hwang, Byung Kook; Ok, Sung Han; Shin, Jeong Sheop.

In: Plant Cell Reports, Vol. 28, No. 3, 01.03.2009, p. 419-427.

Research output: Contribution to journalArticle

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