Overexpression of malic enzyme in the larval stage extends Drosophila lifespan

Gye Hyeong Kim, Young Eun Lee, Gun Ho Lee, Youn Ho Cho, Young Nam Lee, Yeogil Jang, Donggi Paik, Joong Jean Park

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    Metabolic modifications during the developmental period can extend longevity. We found that malic enzyme (Men) overexpression during the larval period lengthened the lifespan of Drosophila. Men overexpression by S106-GeneSwitch-Gal4 driver increased pyruvate content and NADPH/NADP+ ratio but reduced triglyceride, glycogen, and ATP levels in the larvae. ROS levels increased unexpectedly in Men-overexpressing larvae. Interestingly, adults exposed to larval Men-overexpression maintained ROS tolerance with enhanced expression levels of glutathione-S-transferase D2 and thioredoxin-2. Our results suggest that metabolic changes mediated by Men during development might be related to the control of ROS tolerance and the longevity of Drosophila.

    Original languageEnglish
    Pages (from-to)676-682
    Number of pages7
    JournalBiochemical and biophysical research communications
    Volume456
    Issue number2
    DOIs
    Publication statusPublished - 2015 Jan 9

    Keywords

    • Drosophila
    • Longevity
    • Malic enzyme
    • Pyruvate
    • ROS

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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