Overexpression of methionine-R-sulfoxide reductases has no influence on fruit fly aging

Valentina A. Shchedrina, Gerd Vorbrüggen, Byung Cheon Lee, Hwa Young Kim, Hadise Kabil, Lawrence G. Harshman, Vadim N. Gladyshev

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Methionine sulfoxide reductases (Msrs) are enzymes that repair oxidized methionine residues in proteins. This function implicated Msrs in antioxidant defense and the regulation of aging. There are two known Msr types in animals: MsrA specific for the reduction of methionine-S-sulfoxide, and MsrB that catalyzes the reduction of methionine-R-sulfoxide. In a previous study, overexpression of MsrA in the nervous system of Drosophila was found to extend lifespan by 70%. Overexpression of MsrA in yeast also extended lifespan, whereas MsrB overexpression did so only under calorie restriction conditions. The effect of MsrB overexpression on lifespan has not yet been characterized in animal model systems. Here, the GAL4-UAS binary system was used to drive overexpression of cytosolic Drosophila MsrB and mitochondrial mouse MsrB2 in whole body, fatbody, and the nervous system of flies. In contrast to MsrA, MsrB overexpression had no consistent effect on the lifespan of fruit flies on either corn meal or sugar yeast diets. Physical activity, fecundity, and stress resistance were also similar in MsrB-overexpressing and control flies. Thus, MsrA and MsrB, the two proteins with similar function in antioxidant protein repair, have different effects on aging in fruit flies.

Original languageEnglish
Pages (from-to)429-443
Number of pages15
JournalMechanisms of Ageing and Development
Volume130
Issue number7
DOIs
Publication statusPublished - 2009 Jul 1
Externally publishedYes

Fingerprint

Methionine Sulfoxide Reductases
Diptera
Fruit
Nervous System
Drosophila
Antioxidants
Yeasts
Proteins
Methionine
Zea mays
Fertility
Meals
Animal Models
Diet
Enzymes

Keywords

  • Aging
  • Drosophila melanogaster
  • Lifespan
  • Methionine sulfoxide reductase
  • Protein repair

ASJC Scopus subject areas

  • Ageing
  • Developmental Biology

Cite this

Shchedrina, V. A., Vorbrüggen, G., Lee, B. C., Kim, H. Y., Kabil, H., Harshman, L. G., & Gladyshev, V. N. (2009). Overexpression of methionine-R-sulfoxide reductases has no influence on fruit fly aging. Mechanisms of Ageing and Development, 130(7), 429-443. https://doi.org/10.1016/j.mad.2009.04.003

Overexpression of methionine-R-sulfoxide reductases has no influence on fruit fly aging. / Shchedrina, Valentina A.; Vorbrüggen, Gerd; Lee, Byung Cheon; Kim, Hwa Young; Kabil, Hadise; Harshman, Lawrence G.; Gladyshev, Vadim N.

In: Mechanisms of Ageing and Development, Vol. 130, No. 7, 01.07.2009, p. 429-443.

Research output: Contribution to journalArticle

Shchedrina, VA, Vorbrüggen, G, Lee, BC, Kim, HY, Kabil, H, Harshman, LG & Gladyshev, VN 2009, 'Overexpression of methionine-R-sulfoxide reductases has no influence on fruit fly aging', Mechanisms of Ageing and Development, vol. 130, no. 7, pp. 429-443. https://doi.org/10.1016/j.mad.2009.04.003
Shchedrina, Valentina A. ; Vorbrüggen, Gerd ; Lee, Byung Cheon ; Kim, Hwa Young ; Kabil, Hadise ; Harshman, Lawrence G. ; Gladyshev, Vadim N. / Overexpression of methionine-R-sulfoxide reductases has no influence on fruit fly aging. In: Mechanisms of Ageing and Development. 2009 ; Vol. 130, No. 7. pp. 429-443.
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