Overexpression of neurogenin induces ectopic expression of HuC in zebrafish

Cheol Hee Kim, Young Ki Bae, Yojiro Yamanaka, Susumu Yamashita, Takashi Shimizu, Ritsuko Fujii, Hae Chul Park, Sang Yeob Yeo, Tae Lin Huh, Masahiko Hibi, Toshio Hirano

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Several basic helix-loop-helix (bHLH) transcription factors are known to be involved in vertebrate neurogenesis. To investigate their roles in zebrafish neurogenesis, we isolated cDNAs for homologues of neurogenin and Math-1/atonal. The transcription of neurogenin was first detectable in zebrafish nervous system at late gastrulation stage. The expression of zebrafish neurogenin precedes and overlaps that of HuC, one of the earliest neuronal precursor markers. Injection of neurogenin mRNA into early stage zebrafish embryos induced ectopic expression of HuC. These results suggest that neurogenin may participate in the generation of HuC-expressing cells, implying its role in neuronal determination in zebrafish.

Original languageEnglish
Pages (from-to)113-116
Number of pages4
JournalNeuroscience Letters
Volume239
Issue number2-3
DOIs
Publication statusPublished - 1997 Dec 15
Externally publishedYes

Fingerprint

Zebrafish
Neurogenesis
Basic Helix-Loop-Helix Transcription Factors
Gastrulation
Nervous System
Vertebrates
Embryonic Structures
Complementary DNA
Ectopic Gene Expression
Messenger RNA
Injections

Keywords

  • HuC
  • Neural plate
  • Neurogenin
  • Neuronal determination
  • ZATH-1
  • Zebrafish

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kim, C. H., Bae, Y. K., Yamanaka, Y., Yamashita, S., Shimizu, T., Fujii, R., ... Hirano, T. (1997). Overexpression of neurogenin induces ectopic expression of HuC in zebrafish. Neuroscience Letters, 239(2-3), 113-116. https://doi.org/10.1016/S0304-3940(97)00908-7

Overexpression of neurogenin induces ectopic expression of HuC in zebrafish. / Kim, Cheol Hee; Bae, Young Ki; Yamanaka, Yojiro; Yamashita, Susumu; Shimizu, Takashi; Fujii, Ritsuko; Park, Hae Chul; Yeo, Sang Yeob; Huh, Tae Lin; Hibi, Masahiko; Hirano, Toshio.

In: Neuroscience Letters, Vol. 239, No. 2-3, 15.12.1997, p. 113-116.

Research output: Contribution to journalArticle

Kim, CH, Bae, YK, Yamanaka, Y, Yamashita, S, Shimizu, T, Fujii, R, Park, HC, Yeo, SY, Huh, TL, Hibi, M & Hirano, T 1997, 'Overexpression of neurogenin induces ectopic expression of HuC in zebrafish', Neuroscience Letters, vol. 239, no. 2-3, pp. 113-116. https://doi.org/10.1016/S0304-3940(97)00908-7
Kim CH, Bae YK, Yamanaka Y, Yamashita S, Shimizu T, Fujii R et al. Overexpression of neurogenin induces ectopic expression of HuC in zebrafish. Neuroscience Letters. 1997 Dec 15;239(2-3):113-116. https://doi.org/10.1016/S0304-3940(97)00908-7
Kim, Cheol Hee ; Bae, Young Ki ; Yamanaka, Yojiro ; Yamashita, Susumu ; Shimizu, Takashi ; Fujii, Ritsuko ; Park, Hae Chul ; Yeo, Sang Yeob ; Huh, Tae Lin ; Hibi, Masahiko ; Hirano, Toshio. / Overexpression of neurogenin induces ectopic expression of HuC in zebrafish. In: Neuroscience Letters. 1997 ; Vol. 239, No. 2-3. pp. 113-116.
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