Overexpression of neurogenin induces ectopic expression of HuC in zebrafish

Cheol Hee Kim; Young Ki Bae; Yojiro Yamanaka; Susumu Yamashita; Takashi Shimizu; Ritsuko Fujii; Hae Chul Park; Sang Yeob Yeo; Tae Lin Huh; Masahiko Hibi; Toshio Hirano

doi:10.1016/S0304-3940(97)00908-7

Overexpression of neurogenin induces ectopic expression of HuC in zebrafish

Cheol Hee Kim, Young Ki Bae, Yojiro Yamanaka, Susumu Yamashita, Takashi Shimizu, Ritsuko Fujii, Hae Chul Park, Sang Yeob Yeo, Tae Lin Huh, Masahiko Hibi, Toshio Hirano

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

Several basic helix-loop-helix (bHLH) transcription factors are known to be involved in vertebrate neurogenesis. To investigate their roles in zebrafish neurogenesis, we isolated cDNAs for homologues of neurogenin and Math-1/atonal. The transcription of neurogenin was first detectable in zebrafish nervous system at late gastrulation stage. The expression of zebrafish neurogenin precedes and overlaps that of HuC, one of the earliest neuronal precursor markers. Injection of neurogenin mRNA into early stage zebrafish embryos induced ectopic expression of HuC. These results suggest that neurogenin may participate in the generation of HuC-expressing cells, implying its role in neuronal determination in zebrafish.

Original language	English
Pages (from-to)	113-116
Number of pages	4
Journal	Neuroscience Letters
Volume	239
Issue number	2-3
DOIs	https://doi.org/10.1016/S0304-3940(97)00908-7
Publication status	Published - 1997 Dec 15
Externally published	Yes

Keywords

HuC
Neural plate
Neurogenin
Neuronal determination
ZATH-1
Zebrafish

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/S0304-3940(97)00908-7

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title = "Overexpression of neurogenin induces ectopic expression of HuC in zebrafish",

abstract = "Several basic helix-loop-helix (bHLH) transcription factors are known to be involved in vertebrate neurogenesis. To investigate their roles in zebrafish neurogenesis, we isolated cDNAs for homologues of neurogenin and Math-1/atonal. The transcription of neurogenin was first detectable in zebrafish nervous system at late gastrulation stage. The expression of zebrafish neurogenin precedes and overlaps that of HuC, one of the earliest neuronal precursor markers. Injection of neurogenin mRNA into early stage zebrafish embryos induced ectopic expression of HuC. These results suggest that neurogenin may participate in the generation of HuC-expressing cells, implying its role in neuronal determination in zebrafish.",

keywords = "HuC, Neural plate, Neurogenin, Neuronal determination, ZATH-1, Zebrafish",

author = "Kim, {Cheol Hee} and Bae, {Young Ki} and Yojiro Yamanaka and Susumu Yamashita and Takashi Shimizu and Ritsuko Fujii and Park, {Hae Chul} and Yeo, {Sang Yeob} and Huh, {Tae Lin} and Masahiko Hibi and Toshio Hirano",

year = "1997",

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doi = "10.1016/S0304-3940(97)00908-7",

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pages = "113--116",

journal = "Neuroscience Letters",

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number = "2-3",

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T1 - Overexpression of neurogenin induces ectopic expression of HuC in zebrafish

AU - Kim, Cheol Hee

AU - Bae, Young Ki

AU - Yamanaka, Yojiro

AU - Yamashita, Susumu

AU - Shimizu, Takashi

AU - Fujii, Ritsuko

AU - Park, Hae Chul

AU - Yeo, Sang Yeob

AU - Huh, Tae Lin

AU - Hibi, Masahiko

AU - Hirano, Toshio

PY - 1997/12/15

Y1 - 1997/12/15

N2 - Several basic helix-loop-helix (bHLH) transcription factors are known to be involved in vertebrate neurogenesis. To investigate their roles in zebrafish neurogenesis, we isolated cDNAs for homologues of neurogenin and Math-1/atonal. The transcription of neurogenin was first detectable in zebrafish nervous system at late gastrulation stage. The expression of zebrafish neurogenin precedes and overlaps that of HuC, one of the earliest neuronal precursor markers. Injection of neurogenin mRNA into early stage zebrafish embryos induced ectopic expression of HuC. These results suggest that neurogenin may participate in the generation of HuC-expressing cells, implying its role in neuronal determination in zebrafish.

AB - Several basic helix-loop-helix (bHLH) transcription factors are known to be involved in vertebrate neurogenesis. To investigate their roles in zebrafish neurogenesis, we isolated cDNAs for homologues of neurogenin and Math-1/atonal. The transcription of neurogenin was first detectable in zebrafish nervous system at late gastrulation stage. The expression of zebrafish neurogenin precedes and overlaps that of HuC, one of the earliest neuronal precursor markers. Injection of neurogenin mRNA into early stage zebrafish embryos induced ectopic expression of HuC. These results suggest that neurogenin may participate in the generation of HuC-expressing cells, implying its role in neuronal determination in zebrafish.

KW - HuC

KW - Neural plate

KW - Neurogenin

KW - Neuronal determination

KW - ZATH-1

KW - Zebrafish

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DO - 10.1016/S0304-3940(97)00908-7

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SN - 0304-3940

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EP - 116

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 2-3

ER -

Overexpression of neurogenin induces ectopic expression of HuC in zebrafish

Abstract

Keywords

ASJC Scopus subject areas

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