Overexpression of the superoxide anion and NADPH oxidase isoforms1 and 4 (NOX1 and NOX4) inallergic nasal mucosa

Joon Hwan Moon, Tae-Hoon Kim, Heung Man Lee, Seung Hoon Lee, Whan Choe, Ha Kyun Kim, Jung Hoon Lee, Kyoung Ho Oh, Sang Hag Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: The purpose of this study was to investigate the expression and distribution of superoxide anion, NADPH oxidase (NOX)1, and NOX4 in healthy, allergic nasal mucosa and nasal polyps to evaluate the possible influence of oxidative stress on the development of allergic rhinitis and nasal polyps. Methods: The expression and distribution of superoxide anion, NOX1 and NOX4 were evaluated in healthy and allergic nasal mucosa and nasal polyps, using dihydroethidium fluorescence, semiquantitative reverse transcriptase-polymerase chain reaction, immunohistochemistry, and Western blot. Results: NOX1 and NOX4 were localized mainly in the epithelial layer, submucosal glands, vascular endothelium, and inflammatory cells in healthy and allergic nasal mucosa and nasal polyps. The cellular source that generated superoxide anion is also localized in the epithelial cells, submucosal glands, vascular endothelium, and inflammatory cells, demonstrating the similar sites of expression of NOX1 and NOX4 in healthy and allergic nasal mucosa and nasal polyps. NOX1 and NOX4 mRNA and proteins and superoxide anions had increased levels of expression in allergic nasal mucosa and nasal polyps compared with healthy nasal mucosa. Conclusions: These results indicate that NOX1 and NOX4 may play an important role in reactive oxygen species production, contributing to the oxidative stress in allergic rhinitis and nasal polyp tissues.

Original languageEnglish
Pages (from-to)370-376
Number of pages7
JournalAmerican Journal of Rhinology and Allergy
Volume23
Issue number4
DOIs
Publication statusPublished - 2009 Sep 24

Fingerprint

Nasal Polyps
Nasal Mucosa
NADPH Oxidase
Superoxides
Vascular Endothelium
Oxidative Stress
Reverse Transcriptase Polymerase Chain Reaction
Reactive Oxygen Species
Fluorescence
Western Blotting
Epithelial Cells
Immunohistochemistry
Messenger RNA

Keywords

  • Allergic nasal mucosa
  • Dihydroethidium fluorescence
  • Healthy nasal mucosa
  • Immunohistochemistry
  • NADPH oxidase
  • NOX1
  • NOX4
  • Semiquantitative RT-PCR
  • Superoxide anion
  • Western blot

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Immunology and Allergy

Cite this

Overexpression of the superoxide anion and NADPH oxidase isoforms1 and 4 (NOX1 and NOX4) inallergic nasal mucosa. / Moon, Joon Hwan; Kim, Tae-Hoon; Lee, Heung Man; Lee, Seung Hoon; Choe, Whan; Kim, Ha Kyun; Lee, Jung Hoon; Oh, Kyoung Ho; Lee, Sang Hag.

In: American Journal of Rhinology and Allergy, Vol. 23, No. 4, 24.09.2009, p. 370-376.

Research output: Contribution to journalArticle

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abstract = "Background: The purpose of this study was to investigate the expression and distribution of superoxide anion, NADPH oxidase (NOX)1, and NOX4 in healthy, allergic nasal mucosa and nasal polyps to evaluate the possible influence of oxidative stress on the development of allergic rhinitis and nasal polyps. Methods: The expression and distribution of superoxide anion, NOX1 and NOX4 were evaluated in healthy and allergic nasal mucosa and nasal polyps, using dihydroethidium fluorescence, semiquantitative reverse transcriptase-polymerase chain reaction, immunohistochemistry, and Western blot. Results: NOX1 and NOX4 were localized mainly in the epithelial layer, submucosal glands, vascular endothelium, and inflammatory cells in healthy and allergic nasal mucosa and nasal polyps. The cellular source that generated superoxide anion is also localized in the epithelial cells, submucosal glands, vascular endothelium, and inflammatory cells, demonstrating the similar sites of expression of NOX1 and NOX4 in healthy and allergic nasal mucosa and nasal polyps. NOX1 and NOX4 mRNA and proteins and superoxide anions had increased levels of expression in allergic nasal mucosa and nasal polyps compared with healthy nasal mucosa. Conclusions: These results indicate that NOX1 and NOX4 may play an important role in reactive oxygen species production, contributing to the oxidative stress in allergic rhinitis and nasal polyp tissues.",
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AB - Background: The purpose of this study was to investigate the expression and distribution of superoxide anion, NADPH oxidase (NOX)1, and NOX4 in healthy, allergic nasal mucosa and nasal polyps to evaluate the possible influence of oxidative stress on the development of allergic rhinitis and nasal polyps. Methods: The expression and distribution of superoxide anion, NOX1 and NOX4 were evaluated in healthy and allergic nasal mucosa and nasal polyps, using dihydroethidium fluorescence, semiquantitative reverse transcriptase-polymerase chain reaction, immunohistochemistry, and Western blot. Results: NOX1 and NOX4 were localized mainly in the epithelial layer, submucosal glands, vascular endothelium, and inflammatory cells in healthy and allergic nasal mucosa and nasal polyps. The cellular source that generated superoxide anion is also localized in the epithelial cells, submucosal glands, vascular endothelium, and inflammatory cells, demonstrating the similar sites of expression of NOX1 and NOX4 in healthy and allergic nasal mucosa and nasal polyps. NOX1 and NOX4 mRNA and proteins and superoxide anions had increased levels of expression in allergic nasal mucosa and nasal polyps compared with healthy nasal mucosa. Conclusions: These results indicate that NOX1 and NOX4 may play an important role in reactive oxygen species production, contributing to the oxidative stress in allergic rhinitis and nasal polyp tissues.

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