Overexpression of the transforming growth factor-β-inducible gene βig- h3 in anterior polar cataracts

Eunjoo H. Lee, Young Seomun, Kyung Hoon Hwang, Jung Eun Kim, In San Kim, Jae Ho Kim, Choun Ki Joo

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

PURPOSE. In anterior polar cataracts and the fibrosis that can occur after cataract surgery, lens epithelial cells synthesize abundant extracellular matrix molecules and transdifferentiate into myofibroblast-like cells. Transforming growth factor (TGF)-β has been implicated as a key player in these cataractous changes. The purpose of this study was to determine whether the TGF-βinducible gene h3 (βig-h3) is expressed in lens epithelial cells from patients with anterior polar cataracts and to test whether βig-h3 is induced by TGF-β in cultured lens epithelial cells. METHODS. Lens epithelial cells attached to the anterior capsules of human cataractous lenses and noncataractous lenses were examined for the expression of βig-h3 mRNA and protein using reverse transcription-polymerase chain reaction and immunohistochemical analyses. The effect of TGF-β on βig-h3 gene expression was also tested in human lens epithelial B-3 cells using Northern and Western blot analyses. RESULTS. βig-h3 mRNA was not detected in lens epithelial cells from patients with clear lenses or patients with nuclear cataracts. Significant expression of mRNA for βig-h3 was observed in lens epithelial cells from patients with anterior polar cataracts. Immunohistochemical analysis using anti-βig-h3 antiserum indicated that βig-h3 protein was present within the subcapsular plaques of anterior polar cataracts. Treatment of human lens epithelial B-3 cells with TGF-β1 led to an increase in βig-h3 mRNA and the secretion of βig-h3 protein into the culture medium. CONCLUSIONS. βig-h3 may serve as a marker for anterior polar cataracts in addition to previously known proteins, fibronectin, type I collagen, and α-smooth muscle actin. The functions of this protein in lens pathology need to be further investigated.

Original languageEnglish
Pages (from-to)1840-1845
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume41
Issue number7
Publication statusPublished - 2000 Jun 26

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ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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