Abstract
PURPOSE. In anterior polar cataracts and the fibrosis that can occur after cataract surgery, lens epithelial cells synthesize abundant extracellular matrix molecules and transdifferentiate into myofibroblast-like cells. Transforming growth factor (TGF)-β has been implicated as a key player in these cataractous changes. The purpose of this study was to determine whether the TGF-βinducible gene h3 (βig-h3) is expressed in lens epithelial cells from patients with anterior polar cataracts and to test whether βig-h3 is induced by TGF-β in cultured lens epithelial cells. METHODS. Lens epithelial cells attached to the anterior capsules of human cataractous lenses and noncataractous lenses were examined for the expression of βig-h3 mRNA and protein using reverse transcription-polymerase chain reaction and immunohistochemical analyses. The effect of TGF-β on βig-h3 gene expression was also tested in human lens epithelial B-3 cells using Northern and Western blot analyses. RESULTS. βig-h3 mRNA was not detected in lens epithelial cells from patients with clear lenses or patients with nuclear cataracts. Significant expression of mRNA for βig-h3 was observed in lens epithelial cells from patients with anterior polar cataracts. Immunohistochemical analysis using anti-βig-h3 antiserum indicated that βig-h3 protein was present within the subcapsular plaques of anterior polar cataracts. Treatment of human lens epithelial B-3 cells with TGF-β1 led to an increase in βig-h3 mRNA and the secretion of βig-h3 protein into the culture medium. CONCLUSIONS. βig-h3 may serve as a marker for anterior polar cataracts in addition to previously known proteins, fibronectin, type I collagen, and α-smooth muscle actin. The functions of this protein in lens pathology need to be further investigated.
| Original language | English |
|---|---|
| Pages (from-to) | 1840-1845 |
| Number of pages | 6 |
| Journal | Investigative Ophthalmology and Visual Science |
| Volume | 41 |
| Issue number | 7 |
| Publication status | Published - 2000 Jun 26 |
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ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience
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Overexpression of the transforming growth factor-β-inducible gene βig- h3 in anterior polar cataracts. / Lee, Eunjoo H.; Seomun, Young; Hwang, Kyung Hoon; Kim, Jung Eun; Kim, In-San; Kim, Jae Ho; Joo, Choun Ki.
In: Investigative Ophthalmology and Visual Science, Vol. 41, No. 7, 26.06.2000, p. 1840-1845.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Overexpression of the transforming growth factor-β-inducible gene βig- h3 in anterior polar cataracts
AU - Lee, Eunjoo H.
AU - Seomun, Young
AU - Hwang, Kyung Hoon
AU - Kim, Jung Eun
AU - Kim, In-San
AU - Kim, Jae Ho
AU - Joo, Choun Ki
PY - 2000/6/26
Y1 - 2000/6/26
N2 - PURPOSE. In anterior polar cataracts and the fibrosis that can occur after cataract surgery, lens epithelial cells synthesize abundant extracellular matrix molecules and transdifferentiate into myofibroblast-like cells. Transforming growth factor (TGF)-β has been implicated as a key player in these cataractous changes. The purpose of this study was to determine whether the TGF-βinducible gene h3 (βig-h3) is expressed in lens epithelial cells from patients with anterior polar cataracts and to test whether βig-h3 is induced by TGF-β in cultured lens epithelial cells. METHODS. Lens epithelial cells attached to the anterior capsules of human cataractous lenses and noncataractous lenses were examined for the expression of βig-h3 mRNA and protein using reverse transcription-polymerase chain reaction and immunohistochemical analyses. The effect of TGF-β on βig-h3 gene expression was also tested in human lens epithelial B-3 cells using Northern and Western blot analyses. RESULTS. βig-h3 mRNA was not detected in lens epithelial cells from patients with clear lenses or patients with nuclear cataracts. Significant expression of mRNA for βig-h3 was observed in lens epithelial cells from patients with anterior polar cataracts. Immunohistochemical analysis using anti-βig-h3 antiserum indicated that βig-h3 protein was present within the subcapsular plaques of anterior polar cataracts. Treatment of human lens epithelial B-3 cells with TGF-β1 led to an increase in βig-h3 mRNA and the secretion of βig-h3 protein into the culture medium. CONCLUSIONS. βig-h3 may serve as a marker for anterior polar cataracts in addition to previously known proteins, fibronectin, type I collagen, and α-smooth muscle actin. The functions of this protein in lens pathology need to be further investigated.
AB - PURPOSE. In anterior polar cataracts and the fibrosis that can occur after cataract surgery, lens epithelial cells synthesize abundant extracellular matrix molecules and transdifferentiate into myofibroblast-like cells. Transforming growth factor (TGF)-β has been implicated as a key player in these cataractous changes. The purpose of this study was to determine whether the TGF-βinducible gene h3 (βig-h3) is expressed in lens epithelial cells from patients with anterior polar cataracts and to test whether βig-h3 is induced by TGF-β in cultured lens epithelial cells. METHODS. Lens epithelial cells attached to the anterior capsules of human cataractous lenses and noncataractous lenses were examined for the expression of βig-h3 mRNA and protein using reverse transcription-polymerase chain reaction and immunohistochemical analyses. The effect of TGF-β on βig-h3 gene expression was also tested in human lens epithelial B-3 cells using Northern and Western blot analyses. RESULTS. βig-h3 mRNA was not detected in lens epithelial cells from patients with clear lenses or patients with nuclear cataracts. Significant expression of mRNA for βig-h3 was observed in lens epithelial cells from patients with anterior polar cataracts. Immunohistochemical analysis using anti-βig-h3 antiserum indicated that βig-h3 protein was present within the subcapsular plaques of anterior polar cataracts. Treatment of human lens epithelial B-3 cells with TGF-β1 led to an increase in βig-h3 mRNA and the secretion of βig-h3 protein into the culture medium. CONCLUSIONS. βig-h3 may serve as a marker for anterior polar cataracts in addition to previously known proteins, fibronectin, type I collagen, and α-smooth muscle actin. The functions of this protein in lens pathology need to be further investigated.
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M3 - Article
C2 - 10845607
AN - SCOPUS:0034043431
VL - 41
SP - 1840
EP - 1845
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
SN - 0146-0404
IS - 7
ER -