Overproduction, purification, and characterization of heat stable aldolase from Methanococcus jannaschii, a hyperthermophic archaea

In-Geol Choi, Chun Seok Cho, Yunje Cho, Yeon Gyu Yu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

An aldolase gene has been cloned from Methanococcus jannaschii. The coding region of the gene has been expressed in E. coli using a pET system to a level of 30% of total cellular proteins. The protein was purified to more than 95% homogeneity by heat treatment and ion exchange chromatography. The protein performed an aldol condensation reaction with glyceraldehyde as substrate and dihydroxyacetone phosphate as a carboxyl donor. The protein was determined to be a type II aldolase which requires the Zn 2+ ion as a metal cofactor. This enzyme has a broad range of optimum pH (7-9) and temperature (50-80°C). It shows strong stability against heat, chemical denaturants, as well as a high percentage of organic solvents. The half-life of this enzyme at 85°C is more than 24 h and it maintains more than 90% of aldolase activity in the presence of 6 M urea, 50% acetonitrile, or 15% isopropyl alcohol.

Original languageEnglish
Pages (from-to)130-134
Number of pages5
JournalJournal of Biochemistry and Molecular Biology
Volume31
Issue number2
Publication statusPublished - 1998 Mar 31
Externally publishedYes

Fingerprint

Methanocaldococcus
Fructose-Bisphosphate Aldolase
Archaea
Purification
Hot Temperature
Proteins
Genes
Dihydroxyacetone Phosphate
Glyceraldehyde
Condensation reactions
2-Propanol
Ion Exchange Chromatography
Enzymes
Chromatography
Organic solvents
Escherichia coli
Half-Life
Urea
Ion exchange
Metals

Keywords

  • Aldolase
  • Fuculose
  • Hyperthermophile
  • Methanococcus jannaschii

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Overproduction, purification, and characterization of heat stable aldolase from Methanococcus jannaschii, a hyperthermophic archaea. / Choi, In-Geol; Cho, Chun Seok; Cho, Yunje; Yu, Yeon Gyu.

In: Journal of Biochemistry and Molecular Biology, Vol. 31, No. 2, 31.03.1998, p. 130-134.

Research output: Contribution to journalArticle

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