Overweight in mice and enhanced adipogenesis in vitro are associated with lack of the hedgehog coreceptor boc

Hye Jin Lee, Shin Bum Jo, Anthony I. Romer, Hyo Jeong Lim, Min Jung Kim, Seung-Hoi Koo, Robert S. Krauss, Jong Sun Kang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Obesity arises from a combination of genetic, environmental, and behavioral factors. However, the processes that regulate white adipose tissue (WAT) expansion at the level of the adipocyte are not well understood. The Hedgehog (HH) pathway plays a conserved role in adipogenesis, inhibiting fat formation in vivo and in vitro, but it has not been shown that mice with reduced HH pathway activity have enhanced adiposity. We report that mice lacking the HH coreceptor BOC displayed age-related overweight and excess WAT. They also displayed alterations in some metabolic parameters but normal food intake. Furthermore, they had an exacerbated response to a high-fat diet, including enhanced weight gain and adipocyte hypertrophy, livers with greater fat accumulation, and elevated expression of genes related to adipogenesis, lipid metabolism, and adipokine production. Cultured Boc(-/-) mouse embryo fibroblasts showed enhanced adipogenesis relative to Boc(+/+) cells, and they expressed reduced levels of HH pathway target genes. Therefore, a loss-of-function mutation in an HH pathway component is associated with WAT accumulation and overweight in mice. Variant alleles of such HH regulators may contribute to WAT accumulation in human individuals with additional genetic or lifestyle-based predisposition to obesity.

Original languageEnglish
Pages (from-to)2092-2103
Number of pages12
JournalDiabetes
Volume64
Issue number6
DOIs
Publication statusPublished - 2015 Jun 1

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Adipogenesis
Hedgehogs
White Adipose Tissue
Adipocytes
Obesity
Fats
Tissue Expansion
Behavioral Genetics
Adipokines
Adiposity
High Fat Diet
Lipid Metabolism
Hypertrophy
Weight Gain
In Vitro Techniques
Life Style
Embryonic Structures
Fibroblasts
Eating
Alleles

ASJC Scopus subject areas

  • Medicine(all)

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Overweight in mice and enhanced adipogenesis in vitro are associated with lack of the hedgehog coreceptor boc. / Lee, Hye Jin; Jo, Shin Bum; Romer, Anthony I.; Lim, Hyo Jeong; Kim, Min Jung; Koo, Seung-Hoi; Krauss, Robert S.; Kang, Jong Sun.

In: Diabetes, Vol. 64, No. 6, 01.06.2015, p. 2092-2103.

Research output: Contribution to journalArticle

Lee, HJ, Jo, SB, Romer, AI, Lim, HJ, Kim, MJ, Koo, S-H, Krauss, RS & Kang, JS 2015, 'Overweight in mice and enhanced adipogenesis in vitro are associated with lack of the hedgehog coreceptor boc', Diabetes, vol. 64, no. 6, pp. 2092-2103. https://doi.org/10.2337/db14-1017
Lee, Hye Jin ; Jo, Shin Bum ; Romer, Anthony I. ; Lim, Hyo Jeong ; Kim, Min Jung ; Koo, Seung-Hoi ; Krauss, Robert S. ; Kang, Jong Sun. / Overweight in mice and enhanced adipogenesis in vitro are associated with lack of the hedgehog coreceptor boc. In: Diabetes. 2015 ; Vol. 64, No. 6. pp. 2092-2103.
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