Abstract
In order to detect and identify ubiquitous lipid raft marker proteins, we isolated lipid rafts from different mouse organs, including the liver, lung, large brain, and kidney, and analyzed their proteins via 2-DE. Many protein spots were determined to be ubiquitous in all of the lipid rafts, and were annotated via LC and MS/MS. Twelve proteins were identified as ubiquitous raft proteins, and most of these were determined to be mitochondrial proteins, including mortalin, prohibitin, voltage-dependent anion channel, ATP synthase, NADH dehydrogenase, and ubiquinol-cytochrome c reductase. Via immunoblotting, these proteins were shown to exist in detergent-resistant lipid rafts prepared using different organ tissues. Since these oxidation-reduction respiratory chains and ATP synthase complex were detected in detergent-resistant lipid raft fractions which had been isolated from the plasma membrane but not from the mitochondria, and found in the cell surface when determined by immunofluoresence and immunohistochemistry, we conclude that plasma membrane lipid rafts might contain oxidation-reduction respiratory chains and ATP synthase complex.
| Original language | English |
|---|---|
| Pages (from-to) | 2444-2453 |
| Number of pages | 10 |
| Journal | Proteomics |
| Volume | 6 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2006 Apr 1 |
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Keywords
- 2-D PAGE
- ATP synthase
- Lipid raft
- Mitochondria
- Oxidation-reduction respiratory chains
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Oxidation-reduction respiratory chains and ATP synthase complex are localized in detergent-resistant lipid rafts. / Kim, Ki Bum; Lee, Joong Won; Chang, Seok Lee; Kim, Bong Woo; Choo, Hyo Jung; Jung, Soon Young; Chi, Sung-Gil; Yoon, Young Sil; Yoon, Gyesoon; Ko, Young-Gyu.
In: Proteomics, Vol. 6, No. 8, 01.04.2006, p. 2444-2453.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oxidation-reduction respiratory chains and ATP synthase complex are localized in detergent-resistant lipid rafts
AU - Kim, Ki Bum
AU - Lee, Joong Won
AU - Chang, Seok Lee
AU - Kim, Bong Woo
AU - Choo, Hyo Jung
AU - Jung, Soon Young
AU - Chi, Sung-Gil
AU - Yoon, Young Sil
AU - Yoon, Gyesoon
AU - Ko, Young-Gyu
PY - 2006/4/1
Y1 - 2006/4/1
N2 - In order to detect and identify ubiquitous lipid raft marker proteins, we isolated lipid rafts from different mouse organs, including the liver, lung, large brain, and kidney, and analyzed their proteins via 2-DE. Many protein spots were determined to be ubiquitous in all of the lipid rafts, and were annotated via LC and MS/MS. Twelve proteins were identified as ubiquitous raft proteins, and most of these were determined to be mitochondrial proteins, including mortalin, prohibitin, voltage-dependent anion channel, ATP synthase, NADH dehydrogenase, and ubiquinol-cytochrome c reductase. Via immunoblotting, these proteins were shown to exist in detergent-resistant lipid rafts prepared using different organ tissues. Since these oxidation-reduction respiratory chains and ATP synthase complex were detected in detergent-resistant lipid raft fractions which had been isolated from the plasma membrane but not from the mitochondria, and found in the cell surface when determined by immunofluoresence and immunohistochemistry, we conclude that plasma membrane lipid rafts might contain oxidation-reduction respiratory chains and ATP synthase complex.
AB - In order to detect and identify ubiquitous lipid raft marker proteins, we isolated lipid rafts from different mouse organs, including the liver, lung, large brain, and kidney, and analyzed their proteins via 2-DE. Many protein spots were determined to be ubiquitous in all of the lipid rafts, and were annotated via LC and MS/MS. Twelve proteins were identified as ubiquitous raft proteins, and most of these were determined to be mitochondrial proteins, including mortalin, prohibitin, voltage-dependent anion channel, ATP synthase, NADH dehydrogenase, and ubiquinol-cytochrome c reductase. Via immunoblotting, these proteins were shown to exist in detergent-resistant lipid rafts prepared using different organ tissues. Since these oxidation-reduction respiratory chains and ATP synthase complex were detected in detergent-resistant lipid raft fractions which had been isolated from the plasma membrane but not from the mitochondria, and found in the cell surface when determined by immunofluoresence and immunohistochemistry, we conclude that plasma membrane lipid rafts might contain oxidation-reduction respiratory chains and ATP synthase complex.
KW - 2-D PAGE
KW - ATP synthase
KW - Lipid raft
KW - Mitochondria
KW - Oxidation-reduction respiratory chains
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UR - http://www.scopus.com/inward/citedby.url?scp=33646246726&partnerID=8YFLogxK
U2 - 10.1002/pmic.200500574
DO - 10.1002/pmic.200500574
M3 - Article
VL - 6
SP - 2444
EP - 2453
JO - Proteomics
JF - Proteomics
SN - 1615-9853
IS - 8
ER -