Oxidation-reduction respiratory chains and ATP synthase complex are localized in detergent-resistant lipid rafts

Ki Bum Kim, Joong Won Lee, Seok Lee Chang, Bong Woo Kim, Hyo Jung Choo, Soon Young Jung, Sung-Gil Chi, Young Sil Yoon, Gyesoon Yoon, Young-Gyu Ko

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

In order to detect and identify ubiquitous lipid raft marker proteins, we isolated lipid rafts from different mouse organs, including the liver, lung, large brain, and kidney, and analyzed their proteins via 2-DE. Many protein spots were determined to be ubiquitous in all of the lipid rafts, and were annotated via LC and MS/MS. Twelve proteins were identified as ubiquitous raft proteins, and most of these were determined to be mitochondrial proteins, including mortalin, prohibitin, voltage-dependent anion channel, ATP synthase, NADH dehydrogenase, and ubiquinol-cytochrome c reductase. Via immunoblotting, these proteins were shown to exist in detergent-resistant lipid rafts prepared using different organ tissues. Since these oxidation-reduction respiratory chains and ATP synthase complex were detected in detergent-resistant lipid raft fractions which had been isolated from the plasma membrane but not from the mitochondria, and found in the cell surface when determined by immunofluoresence and immunohistochemistry, we conclude that plasma membrane lipid rafts might contain oxidation-reduction respiratory chains and ATP synthase complex.

Original languageEnglish
Pages (from-to)2444-2453
Number of pages10
JournalProteomics
Volume6
Issue number8
DOIs
Publication statusPublished - 2006 Apr 1

Fingerprint

Electron Transport
Detergents
Oxidation-Reduction
Adenosine Triphosphate
Lipids
Proteins
Cell membranes
Voltage-Dependent Anion Channels
Cell Membrane
NADH Dehydrogenase
Mitochondria
Electron Transport Complex III
Mitochondrial Proteins
Membrane Lipids
Immunoblotting
Liver
Brain
Immunohistochemistry
Tissue
Kidney

Keywords

  • 2-D PAGE
  • ATP synthase
  • Lipid raft
  • Mitochondria
  • Oxidation-reduction respiratory chains

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Oxidation-reduction respiratory chains and ATP synthase complex are localized in detergent-resistant lipid rafts. / Kim, Ki Bum; Lee, Joong Won; Chang, Seok Lee; Kim, Bong Woo; Choo, Hyo Jung; Jung, Soon Young; Chi, Sung-Gil; Yoon, Young Sil; Yoon, Gyesoon; Ko, Young-Gyu.

In: Proteomics, Vol. 6, No. 8, 01.04.2006, p. 2444-2453.

Research output: Contribution to journalArticle

Kim, Ki Bum ; Lee, Joong Won ; Chang, Seok Lee ; Kim, Bong Woo ; Choo, Hyo Jung ; Jung, Soon Young ; Chi, Sung-Gil ; Yoon, Young Sil ; Yoon, Gyesoon ; Ko, Young-Gyu. / Oxidation-reduction respiratory chains and ATP synthase complex are localized in detergent-resistant lipid rafts. In: Proteomics. 2006 ; Vol. 6, No. 8. pp. 2444-2453.
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