Oxidative stress and apoptosis induced by ZnO nanoparticles in HaCaT cells

Hyun Cheol Bae, Hwa Jung Ryu, Sang Hoon Jeong, Eun Young Lee, Yoon Hee Park, Kyung Goo Lee, Byeong Hyeok Choi, Eun Ho Maeng, Meyoung-Kon Kim, Sang Wook Son

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Zinc oxide (ZnO) nanoparticles (NPs) are used in the cosmetic industry in cosmetics and sunscreen. ZnO NPs have been reported to elicit various adverse cellular effects, including cytotoxicity. However, the underlying mechanisms of these adverse effects have not been fully characterized. To investigate the potential of cytotoxicity induced by ZnO NPs, we evaluated cytosolic reactive oxygen species levels in human keratinocyte HaCaT cells treated with ZnO NPs having different surface charges and particle sizes. A short period of treatment (30 min) with 100 nm ZnO NPs resulted in a greater increase of cytosolic ROS levels, compared to treatment with 20 nm ZnO NPs at the same concentration. During a long period of treatment (24 h) with ZnO NPs, intracellular ROS was increased in cells treated with 20 μg/mL 20 nm (+/-) charged ZnO. No significant difference according to differences in surface charge was observed. In addition, total levels of caspase-7 and PARP were decreased by ZnO NPs. These results demonstrated that ZnO NPs could induce ROS mediated apoptosis.

Original languageEnglish
Pages (from-to)333-337
Number of pages5
JournalMolecular and Cellular Toxicology
Volume7
Issue number4
DOIs
Publication statusPublished - 2011 Dec 1

Fingerprint

Zinc Oxide
Oxidative stress
Nanoparticles
Oxidative Stress
Apoptosis
Cosmetics
Cytotoxicity
Surface charge
Sun hoods
Caspase 7
Sunscreening Agents
Keratinocytes
Particle Size
Reactive Oxygen Species
Industry
Particle size

Keywords

  • Cytotoxicity
  • Keratinocyte
  • ROS
  • ZnO

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health
  • Pathology and Forensic Medicine

Cite this

Oxidative stress and apoptosis induced by ZnO nanoparticles in HaCaT cells. / Bae, Hyun Cheol; Ryu, Hwa Jung; Jeong, Sang Hoon; Lee, Eun Young; Park, Yoon Hee; Lee, Kyung Goo; Choi, Byeong Hyeok; Maeng, Eun Ho; Kim, Meyoung-Kon; Son, Sang Wook.

In: Molecular and Cellular Toxicology, Vol. 7, No. 4, 01.12.2011, p. 333-337.

Research output: Contribution to journalArticle

Bae, Hyun Cheol ; Ryu, Hwa Jung ; Jeong, Sang Hoon ; Lee, Eun Young ; Park, Yoon Hee ; Lee, Kyung Goo ; Choi, Byeong Hyeok ; Maeng, Eun Ho ; Kim, Meyoung-Kon ; Son, Sang Wook. / Oxidative stress and apoptosis induced by ZnO nanoparticles in HaCaT cells. In: Molecular and Cellular Toxicology. 2011 ; Vol. 7, No. 4. pp. 333-337.
@article{630202b384d641158cc09e3bb490c27c,
title = "Oxidative stress and apoptosis induced by ZnO nanoparticles in HaCaT cells",
abstract = "Zinc oxide (ZnO) nanoparticles (NPs) are used in the cosmetic industry in cosmetics and sunscreen. ZnO NPs have been reported to elicit various adverse cellular effects, including cytotoxicity. However, the underlying mechanisms of these adverse effects have not been fully characterized. To investigate the potential of cytotoxicity induced by ZnO NPs, we evaluated cytosolic reactive oxygen species levels in human keratinocyte HaCaT cells treated with ZnO NPs having different surface charges and particle sizes. A short period of treatment (30 min) with 100 nm ZnO NPs resulted in a greater increase of cytosolic ROS levels, compared to treatment with 20 nm ZnO NPs at the same concentration. During a long period of treatment (24 h) with ZnO NPs, intracellular ROS was increased in cells treated with 20 μg/mL 20 nm (+/-) charged ZnO. No significant difference according to differences in surface charge was observed. In addition, total levels of caspase-7 and PARP were decreased by ZnO NPs. These results demonstrated that ZnO NPs could induce ROS mediated apoptosis.",
keywords = "Cytotoxicity, Keratinocyte, ROS, ZnO",
author = "Bae, {Hyun Cheol} and Ryu, {Hwa Jung} and Jeong, {Sang Hoon} and Lee, {Eun Young} and Park, {Yoon Hee} and Lee, {Kyung Goo} and Choi, {Byeong Hyeok} and Maeng, {Eun Ho} and Meyoung-Kon Kim and Son, {Sang Wook}",
year = "2011",
month = "12",
day = "1",
doi = "10.1007/s13273-011-0042-9",
language = "English",
volume = "7",
pages = "333--337",
journal = "Molecular and Cellular Toxicology",
issn = "1738-642X",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - Oxidative stress and apoptosis induced by ZnO nanoparticles in HaCaT cells

AU - Bae, Hyun Cheol

AU - Ryu, Hwa Jung

AU - Jeong, Sang Hoon

AU - Lee, Eun Young

AU - Park, Yoon Hee

AU - Lee, Kyung Goo

AU - Choi, Byeong Hyeok

AU - Maeng, Eun Ho

AU - Kim, Meyoung-Kon

AU - Son, Sang Wook

PY - 2011/12/1

Y1 - 2011/12/1

N2 - Zinc oxide (ZnO) nanoparticles (NPs) are used in the cosmetic industry in cosmetics and sunscreen. ZnO NPs have been reported to elicit various adverse cellular effects, including cytotoxicity. However, the underlying mechanisms of these adverse effects have not been fully characterized. To investigate the potential of cytotoxicity induced by ZnO NPs, we evaluated cytosolic reactive oxygen species levels in human keratinocyte HaCaT cells treated with ZnO NPs having different surface charges and particle sizes. A short period of treatment (30 min) with 100 nm ZnO NPs resulted in a greater increase of cytosolic ROS levels, compared to treatment with 20 nm ZnO NPs at the same concentration. During a long period of treatment (24 h) with ZnO NPs, intracellular ROS was increased in cells treated with 20 μg/mL 20 nm (+/-) charged ZnO. No significant difference according to differences in surface charge was observed. In addition, total levels of caspase-7 and PARP were decreased by ZnO NPs. These results demonstrated that ZnO NPs could induce ROS mediated apoptosis.

AB - Zinc oxide (ZnO) nanoparticles (NPs) are used in the cosmetic industry in cosmetics and sunscreen. ZnO NPs have been reported to elicit various adverse cellular effects, including cytotoxicity. However, the underlying mechanisms of these adverse effects have not been fully characterized. To investigate the potential of cytotoxicity induced by ZnO NPs, we evaluated cytosolic reactive oxygen species levels in human keratinocyte HaCaT cells treated with ZnO NPs having different surface charges and particle sizes. A short period of treatment (30 min) with 100 nm ZnO NPs resulted in a greater increase of cytosolic ROS levels, compared to treatment with 20 nm ZnO NPs at the same concentration. During a long period of treatment (24 h) with ZnO NPs, intracellular ROS was increased in cells treated with 20 μg/mL 20 nm (+/-) charged ZnO. No significant difference according to differences in surface charge was observed. In addition, total levels of caspase-7 and PARP were decreased by ZnO NPs. These results demonstrated that ZnO NPs could induce ROS mediated apoptosis.

KW - Cytotoxicity

KW - Keratinocyte

KW - ROS

KW - ZnO

UR - http://www.scopus.com/inward/record.url?scp=84863063470&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863063470&partnerID=8YFLogxK

U2 - 10.1007/s13273-011-0042-9

DO - 10.1007/s13273-011-0042-9

M3 - Article

VL - 7

SP - 333

EP - 337

JO - Molecular and Cellular Toxicology

JF - Molecular and Cellular Toxicology

SN - 1738-642X

IS - 4

ER -