Oxygen-evolving enhancer protein 2 is phosphorylated by glycine-rich protein 3/wall-associated kinase 1 in Arabidopsis

Eun Ju Yang, Young Ah Oh, Eui Seung Lee, Ae Ran Park, Somi K. Cho, Yung Joon Yoo, Ohkmae Kim

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The Arabidopsis wall-associated receptor kinase, WAK1, is a member of WAK family that links the plasma membrane to the extracellular matrix. A glycine-rich secreted protein, AtGRP-3, was previously shown to regulate WAK1 functions through binding to the extracellular domain of WAK1. In this study, we sought to determine the downstream molecules of the AtGRP-3/WAK1 signaling pathway, by using two-dimensional gel electrophoresis combined with Edman sequencing and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). We report here that a chloroplast protein, oxygen-evolving enhancer protein 2 (OEE2), specifically interacts with the cytoplasmic kinase domain of WAK1 and becomes phosphorylated in an AtGRP-3-dependent manner. The phosphorylation of OEE2 is also induced in Arabidopsis by treatment with avirulent Pseudomonas syringae. Taken together, these results suggest that OEE2 activity is regulated by AtGRP-3/WAK1.

Original languageEnglish
Pages (from-to)862-868
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume305
Issue number4
DOIs
Publication statusPublished - 2003 Jun 13
Externally publishedYes

Fingerprint

Arabidopsis
Glycine
Phosphotransferases
Oxygen
Proteins
Chloroplast Proteins
Pseudomonas syringae
Phosphorylation
Electrophoresis, Gel, Two-Dimensional
Cell membranes
Electrophoresis
Ionization
Extracellular Matrix
Mass spectrometry
Desorption
Mass Spectrometry
Lasers
Gels
Cell Membrane
Molecules

Keywords

  • Arabidopsis
  • AtGRP-3
  • Kinase
  • OEE2
  • Receptor
  • Signal transduction
  • WAK1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Oxygen-evolving enhancer protein 2 is phosphorylated by glycine-rich protein 3/wall-associated kinase 1 in Arabidopsis. / Yang, Eun Ju; Oh, Young Ah; Lee, Eui Seung; Park, Ae Ran; Cho, Somi K.; Yoo, Yung Joon; Kim, Ohkmae.

In: Biochemical and Biophysical Research Communications, Vol. 305, No. 4, 13.06.2003, p. 862-868.

Research output: Contribution to journalArticle

Yang, Eun Ju ; Oh, Young Ah ; Lee, Eui Seung ; Park, Ae Ran ; Cho, Somi K. ; Yoo, Yung Joon ; Kim, Ohkmae. / Oxygen-evolving enhancer protein 2 is phosphorylated by glycine-rich protein 3/wall-associated kinase 1 in Arabidopsis. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 305, No. 4. pp. 862-868.
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