Oxygen transfer correlation in high cell density culture of recombinant E. coli

Chul Soo Shin, Min Sun Hong, Jeewon Lee

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A recombinant E. coli BL21[pET3a-T2M2] was cultivated in fed-batch cultures and cell mass increased to more than 70g/L. The volumetric oxygen transfer coefficient was estimated in a range of various fermentation parameters (agitation speed, oxygen flow rate and cell mass concentration) and finally the oxygen transfer correlation in bioreactor containing the recombinant E. coli cultures was determined as: k(L)a = 0.0195 (P(g)/V)0.55 (V3)0.64 (1 + 2.12X + 0.20X2)-0.25.

Original languageEnglish
Pages (from-to)679-682
Number of pages4
JournalBiotechnology Techniques
Volume10
Issue number9
Publication statusPublished - 1996
Externally publishedYes

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Escherichia coli
Cell Culture Techniques
Cell Count
Oxygen
Batch Cell Culture Techniques
Bioreactors
Fermentation
Flow rate

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry

Cite this

Oxygen transfer correlation in high cell density culture of recombinant E. coli. / Shin, Chul Soo; Hong, Min Sun; Lee, Jeewon.

In: Biotechnology Techniques, Vol. 10, No. 9, 1996, p. 679-682.

Research output: Contribution to journalArticle

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