Abstract
Oxymatrine is a traditional Chinese herbal product that exhibits anti-inflammatory effects in models of heart, brain and liver injury. We investigated the impact of oxymatrine in an acute model of intestinal injury and inflammation. Oxymatrine significantly decreased LPS-induced NF-κB-driven luciferase activity, correlating with diminished induction of Cxcl2, Tnfaand Il6mRNA expression in rat IEC-6 and murine BMDC. Although oxymatrine decreased LPS-induced p65 nuclear translocation and binding to the Cxcl2 gene promoter, this effect was independent of IκBα degradation/phosphorylation. DSS-induced weight loss and histological damage were ameliorated in oxymatrine-treated C57BL/6-WT-mice. While this effect correlated with reduced colonic Il6and Il1βmRNA accumulation, global NF-κB activity as measured in NF-κBEGFP mice was unaffected. Our data demonstrate that oxymatrine reduces LPS-induced NF-κB nuclear translocation and activity independently of IκBα status, prevents intestinal inflammation through blockade of inflammatory signaling and ameliorates overall intestinal inflammation in vivo.
| Original language | English |
|---|---|
| Article number | 01629 |
| Journal | Scientific Reports |
| Volume | 3 |
| DOIs | |
| Publication status | Published - 2013 Apr 9 |
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Oxymatrine prevents NF-κB nuclear translocation and ameliorates acute intestinal inflammation. / Guzman, Javier Rivera; Koo, Ja Seol; Goldsmith, Jason R.; Mühlbauer, Marcus; Narula, Acharan; Jobin, Christian.
In: Scientific Reports, Vol. 3, 01629, 09.04.2013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oxymatrine prevents NF-κB nuclear translocation and ameliorates acute intestinal inflammation
AU - Guzman, Javier Rivera
AU - Koo, Ja Seol
AU - Goldsmith, Jason R.
AU - Mühlbauer, Marcus
AU - Narula, Acharan
AU - Jobin, Christian
PY - 2013/4/9
Y1 - 2013/4/9
N2 - Oxymatrine is a traditional Chinese herbal product that exhibits anti-inflammatory effects in models of heart, brain and liver injury. We investigated the impact of oxymatrine in an acute model of intestinal injury and inflammation. Oxymatrine significantly decreased LPS-induced NF-κB-driven luciferase activity, correlating with diminished induction of Cxcl2, Tnfaand Il6mRNA expression in rat IEC-6 and murine BMDC. Although oxymatrine decreased LPS-induced p65 nuclear translocation and binding to the Cxcl2 gene promoter, this effect was independent of IκBα degradation/phosphorylation. DSS-induced weight loss and histological damage were ameliorated in oxymatrine-treated C57BL/6-WT-mice. While this effect correlated with reduced colonic Il6and Il1βmRNA accumulation, global NF-κB activity as measured in NF-κBEGFP mice was unaffected. Our data demonstrate that oxymatrine reduces LPS-induced NF-κB nuclear translocation and activity independently of IκBα status, prevents intestinal inflammation through blockade of inflammatory signaling and ameliorates overall intestinal inflammation in vivo.
AB - Oxymatrine is a traditional Chinese herbal product that exhibits anti-inflammatory effects in models of heart, brain and liver injury. We investigated the impact of oxymatrine in an acute model of intestinal injury and inflammation. Oxymatrine significantly decreased LPS-induced NF-κB-driven luciferase activity, correlating with diminished induction of Cxcl2, Tnfaand Il6mRNA expression in rat IEC-6 and murine BMDC. Although oxymatrine decreased LPS-induced p65 nuclear translocation and binding to the Cxcl2 gene promoter, this effect was independent of IκBα degradation/phosphorylation. DSS-induced weight loss and histological damage were ameliorated in oxymatrine-treated C57BL/6-WT-mice. While this effect correlated with reduced colonic Il6and Il1βmRNA accumulation, global NF-κB activity as measured in NF-κBEGFP mice was unaffected. Our data demonstrate that oxymatrine reduces LPS-induced NF-κB nuclear translocation and activity independently of IκBα status, prevents intestinal inflammation through blockade of inflammatory signaling and ameliorates overall intestinal inflammation in vivo.
UR - http://www.scopus.com/inward/record.url?scp=84895095291&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84895095291&partnerID=8YFLogxK
U2 - 10.1038/srep01629
DO - 10.1038/srep01629
M3 - Article
C2 - 23568217
AN - SCOPUS:84895095291
VL - 3
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 01629
ER -