Oxymatrine prevents NF-κB nuclear translocation and ameliorates acute intestinal inflammation

Javier Rivera Guzman, Ja Seol Koo, Jason R. Goldsmith, Marcus Mühlbauer, Acharan Narula, Christian Jobin

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74 Citations (Scopus)

Abstract

Oxymatrine is a traditional Chinese herbal product that exhibits anti-inflammatory effects in models of heart, brain and liver injury. We investigated the impact of oxymatrine in an acute model of intestinal injury and inflammation. Oxymatrine significantly decreased LPS-induced NF-κB-driven luciferase activity, correlating with diminished induction of Cxcl2, Tnfaand Il6mRNA expression in rat IEC-6 and murine BMDC. Although oxymatrine decreased LPS-induced p65 nuclear translocation and binding to the Cxcl2 gene promoter, this effect was independent of IκBα degradation/phosphorylation. DSS-induced weight loss and histological damage were ameliorated in oxymatrine-treated C57BL/6-WT-mice. While this effect correlated with reduced colonic Il6and Il1βmRNA accumulation, global NF-κB activity as measured in NF-κBEGFP mice was unaffected. Our data demonstrate that oxymatrine reduces LPS-induced NF-κB nuclear translocation and activity independently of IκBα status, prevents intestinal inflammation through blockade of inflammatory signaling and ameliorates overall intestinal inflammation in vivo.

Original languageEnglish
Article number1629
JournalScientific reports
Volume3
DOIs
Publication statusPublished - 2013 Apr 9

ASJC Scopus subject areas

  • General

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