Oxyresveratrol improves tight junction integrity through the PKC and MAPK signaling pathways in Caco-2 cells

Hyun A. Jo, Dahyun Hwang, Jeong Keun Kim, Young Hee Lim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Strengthening intestinal tight junctions (TJ) provides an effective barrier from the external environment and is important for recovery from inflammatory bowel disease. Oxyresveratrol (OXY), an isomer of hydroxylated resveratrol, is isolated from many plants. The aim of this study was to investigate the effect of OXY on intestinal TJ and to elucidate the mechanism underlying the OXY-mediated increase in TJ integrity in human intestinal Caco-2 cells. OXY-treated Caco-2 cell monolayers showed decreased monolayer permeability as evaluated by paracellular transport assay. The results showed that OXY significantly increased the levels of TJ-related genes and proteins (Claudin-1, Occludin and ZO-1) compared with those of the negative control. OXY activated protein kinase C (PKC) and increased expression levels of mitogen-activated protein kinase (MAPK) genes. OXY also increased gene and protein levels of the transcription factor Cdx-2. Expression levels of TJ, PKC and Cdx-2 proteins and transepithelial electrical resistance (TEER) value decreased in OXY-treated Caco-2 cells following treatment with a pan-PKC inhibitor compared with those of the untreated control. In conclusion, OXY strengthens the integrity of the intestinal TJ barrier via activation of the PKC and MAPK pathways.

Original languageEnglish
Pages (from-to)203-213
Number of pages11
JournalFood and Chemical Toxicology
Volume108
DOIs
Publication statusPublished - 2017 Oct 1

Fingerprint

tight junctions
Tight Junctions
protein kinase C
Mitogen-Activated Protein Kinases
mitogen-activated protein kinase
Protein Kinase C
Caco-2 Cells
electrical resistance
genes
proteins
inflammatory bowel disease
Monolayers
resveratrol
cells
Claudin-1
Occludin
Tight Junction Proteins
isomers
puag-haad
permeability

Keywords

  • Intestinal permeability
  • Mitogen-activated protein kinase
  • Oxyresveratrol
  • Protein kinase C
  • Tight junctions

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Oxyresveratrol improves tight junction integrity through the PKC and MAPK signaling pathways in Caco-2 cells. / Jo, Hyun A.; Hwang, Dahyun; Kim, Jeong Keun; Lim, Young Hee.

In: Food and Chemical Toxicology, Vol. 108, 01.10.2017, p. 203-213.

Research output: Contribution to journalArticle

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