Oxyresveratrol stimulates mucin production in an NAD+-dependent manner in human intestinal goblet cells

Dahyun Hwang, Hyun A. Jo, Seong Ho Ma, Young Hee Lim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The intestinal mucus layer plays an important role in the management of inflammatory bowel disease. The aim of this study was to investigate the effects of oxyresveratrol (OXY), an antioxidant, on the stimulation of mucin production in human LS 174T goblet cells and the underlying mechanism thereof. OXY increased MUC2 expression at both the mRNA and protein levels. By performing two-dimensional gel electrophoresis, we found that the expression of nicotinic acid phosphoribosyltransferase1 (NaPRT1) in OXY-treated LS 174T cells was greatly increased compared with that in negative control cells. In addition, the NAD+/NADH ratio was increased in proportion to OXY in LS 174T cells. The expression of NAD+-synthesis enzymes, NaPRT1, nicotinamide riboside kinase1 (NRK1) and nicotinamide mononucleotide adenylyltransferase1 (Nmnat1) was significantly increased at both the mRNA and protein levels in OXY-treated LS 174T cells. The inhibition of NaPRT1 and NRK1 did not decrease MUC2 expression after inhibiting by small interfering RNA (siRNA)-NaPRT1 and siRNA-NRK1, respectively; however, inhibition of Nmnat by an Nmnat inhibitor decreased MUC2 expression in a dose-dependent manner. In conclusion, OXY increases NAD+ levels, resulting in the stimulation of MUC2 expression in LS 174T cells. These findings present a novel role for NAD+ in stimulation of MUC2 expression.

Original languageEnglish
Pages (from-to)880-888
Number of pages9
JournalFood and Chemical Toxicology
Volume118
DOIs
Publication statusPublished - 2018 Aug 1

Fingerprint

Goblet Cells
goblet cells
mucins
Mucins
nicotinamide
NAD
niacin
Niacin
small interfering RNA
cells
Small Interfering RNA
Nicotinamide Mononucleotide
inflammatory bowel disease
two-dimensional gel electrophoresis
Messenger RNA
mucus
Electrophoresis, Gel, Two-Dimensional
Mucus
Electrophoresis
Inflammatory Bowel Diseases

Keywords

  • Goblet cells
  • Intestinal mucus layer
  • MUC2
  • NAD
  • Oxyresveratrol

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Oxyresveratrol stimulates mucin production in an NAD+-dependent manner in human intestinal goblet cells. / Hwang, Dahyun; Jo, Hyun A.; Ma, Seong Ho; Lim, Young Hee.

In: Food and Chemical Toxicology, Vol. 118, 01.08.2018, p. 880-888.

Research output: Contribution to journalArticle

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