Oxytocin produces thermal analgesia via vasopressin-1a receptor by modulating TRPV1 and potassium conductance in the dorsal root ganglion neurons

Rafael Taeho Han, Han Byul Kim, Young Beom Kim, Kyungmin Choi, Gi Yeon Park, Pa Reum Lee, Jaehee Lee, Hye Young Kim, Chul Kyu Park, Youngnam Kang, Seog Bae Oh, Heung Sik Na

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Recent studies have provided several lines of evidence that peripheral administration of oxytocin induces analgesia in human and rodents. However, the exact underlying mechanism of analgesia still remains elusive. In the present study, we aimed to identify which receptor could mediate the analgesic effect of intraperitoneal injection of oxytocin and its cellular mechanisms in thermal pain behavior. We found that oxytocin-induced analgesia could be reversed by d(CH2)5[Tyr(Me)2, Dab5] AVP, a vasopressin-1a (V1a) receptor antagonist, but not by desGly-NH2-d(CH2)5[DTyr2, Thr4]OVT, an oxytocin receptor antagonist. Single cell RT-PCR analysis revealed that V1a receptor, compared to oxytocin, vasopressin-1b and vasopressin-2 receptors, was more profoundly expressed in dorsal root ganglion (DRG) neurons and the expression of V1a receptor was predominant in transient receptor potential vanilloid 1 (TRPV1)-expressing DRG neurons. Fura-2 based calcium imaging experiments showed that capsaicin-induced calcium transient was significantly inhibited by oxytocin and that such inhibition was reversed by V1a receptor antagonist. Additionally, whole cell patch clamp recording demonstrated that oxytocin significantly increased potassium conductance via V1a receptor in DRG neurons. Taken together, our findings suggest that analgesic effects produced by peripheral administration of oxytocin were attributable to the activation of V1a receptor, resulting in reduction of TRPV1 activity and enhancement of potassium conductance in DRG neurons.

Original languageEnglish
Pages (from-to)173-182
Number of pages10
JournalKorean Journal of Physiology and Pharmacology
Volume22
Issue number2
DOIs
Publication statusPublished - 2018 Mar 1

Fingerprint

Vasopressin Receptors
Spinal Ganglia
Oxytocin
Analgesia
Potassium
Hot Temperature
Neurons
Analgesics
Oxytocin Receptors
Calcium
Fura-2
Capsaicin
vanilloid receptor subtype 1
Intraperitoneal Injections
Vasopressins
Rodentia
Pain
Polymerase Chain Reaction

Keywords

  • Dorsal root ganglion
  • Electrophysiology
  • Oxytocin
  • Pain
  • Vasopressin receptor

ASJC Scopus subject areas

  • Physiology
  • Pharmacology

Cite this

Oxytocin produces thermal analgesia via vasopressin-1a receptor by modulating TRPV1 and potassium conductance in the dorsal root ganglion neurons. / Han, Rafael Taeho; Kim, Han Byul; Kim, Young Beom; Choi, Kyungmin; Park, Gi Yeon; Lee, Pa Reum; Lee, Jaehee; Kim, Hye Young; Park, Chul Kyu; Kang, Youngnam; Oh, Seog Bae; Na, Heung Sik.

In: Korean Journal of Physiology and Pharmacology, Vol. 22, No. 2, 01.03.2018, p. 173-182.

Research output: Contribution to journalArticle

Han, Rafael Taeho ; Kim, Han Byul ; Kim, Young Beom ; Choi, Kyungmin ; Park, Gi Yeon ; Lee, Pa Reum ; Lee, Jaehee ; Kim, Hye Young ; Park, Chul Kyu ; Kang, Youngnam ; Oh, Seog Bae ; Na, Heung Sik. / Oxytocin produces thermal analgesia via vasopressin-1a receptor by modulating TRPV1 and potassium conductance in the dorsal root ganglion neurons. In: Korean Journal of Physiology and Pharmacology. 2018 ; Vol. 22, No. 2. pp. 173-182.
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AU - Han, Rafael Taeho

AU - Kim, Han Byul

AU - Kim, Young Beom

AU - Choi, Kyungmin

AU - Park, Gi Yeon

AU - Lee, Pa Reum

AU - Lee, Jaehee

AU - Kim, Hye Young

AU - Park, Chul Kyu

AU - Kang, Youngnam

AU - Oh, Seog Bae

AU - Na, Heung Sik

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KW - Pain

KW - Vasopressin receptor

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