Oxytocin stimulates glucose uptake in skeletal muscle cells through the calcium-CaMKK-AMPK pathway

Eun Soo Lee, Kyung Ok Uhm, Yun Mi Lee, Joseph Kwon, Sun-Hwa Park, Hyeon Soo Kim

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Oxytocin is a mammalian hormone that is released mainly after distension of the uterine cervix. In this study, we report that oxytocin stimulates intracellular release of calcium, and also activates AMPK (AMP-activated protein kinase) in C2C12 myoblast cells in a time/dose-dependent manner. Oxytocin receptor mRNA was detected in C2C12 cells. In addition, oxytocin stimulated glucose uptake and, moreover, inhibition of either CaMKK (Ca2+/calmodulin-dependent protein kinase kinase) or AMPK blocked oxytocin-mediated AMPK activation and glucose uptake. Taken together, our findings suggest that oxytocin may serve a peripheral metabolic function in skeletal muscle cells through the calcium-CaMKK-AMPK pathway.

Original languageEnglish
Pages (from-to)71-74
Number of pages4
JournalRegulatory Peptides
Volume151
Issue number1-3
DOIs
Publication statusPublished - 2008 Nov 29

Fingerprint

Calcium-Calmodulin-Dependent Protein Kinase Kinase
Calcium-Calmodulin-Dependent Protein Kinases
Oxytocin
Muscle Cells
Muscle
Skeletal Muscle
Cells
AMP-Activated Protein Kinases
Calcium
Glucose
Oxytocin Receptors
Myoblasts
Cervix Uteri
Phosphotransferases
Chemical activation
AMP-activated protein kinase kinase
Hormones
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Oxytocin stimulates glucose uptake in skeletal muscle cells through the calcium-CaMKK-AMPK pathway. / Lee, Eun Soo; Uhm, Kyung Ok; Lee, Yun Mi; Kwon, Joseph; Park, Sun-Hwa; Kim, Hyeon Soo.

In: Regulatory Peptides, Vol. 151, No. 1-3, 29.11.2008, p. 71-74.

Research output: Contribution to journalArticle

Lee, Eun Soo ; Uhm, Kyung Ok ; Lee, Yun Mi ; Kwon, Joseph ; Park, Sun-Hwa ; Kim, Hyeon Soo. / Oxytocin stimulates glucose uptake in skeletal muscle cells through the calcium-CaMKK-AMPK pathway. In: Regulatory Peptides. 2008 ; Vol. 151, No. 1-3. pp. 71-74.
@article{c238277bac88420683366502af099418,
title = "Oxytocin stimulates glucose uptake in skeletal muscle cells through the calcium-CaMKK-AMPK pathway",
abstract = "Oxytocin is a mammalian hormone that is released mainly after distension of the uterine cervix. In this study, we report that oxytocin stimulates intracellular release of calcium, and also activates AMPK (AMP-activated protein kinase) in C2C12 myoblast cells in a time/dose-dependent manner. Oxytocin receptor mRNA was detected in C2C12 cells. In addition, oxytocin stimulated glucose uptake and, moreover, inhibition of either CaMKK (Ca2+/calmodulin-dependent protein kinase kinase) or AMPK blocked oxytocin-mediated AMPK activation and glucose uptake. Taken together, our findings suggest that oxytocin may serve a peripheral metabolic function in skeletal muscle cells through the calcium-CaMKK-AMPK pathway.",
keywords = "ACC, AMPK, Calcium, CaMKK, Oxytocin",
author = "Lee, {Eun Soo} and Uhm, {Kyung Ok} and Lee, {Yun Mi} and Joseph Kwon and Sun-Hwa Park and Kim, {Hyeon Soo}",
year = "2008",
month = "11",
day = "29",
doi = "10.1016/j.regpep.2008.05.001",
language = "English",
volume = "151",
pages = "71--74",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - Oxytocin stimulates glucose uptake in skeletal muscle cells through the calcium-CaMKK-AMPK pathway

AU - Lee, Eun Soo

AU - Uhm, Kyung Ok

AU - Lee, Yun Mi

AU - Kwon, Joseph

AU - Park, Sun-Hwa

AU - Kim, Hyeon Soo

PY - 2008/11/29

Y1 - 2008/11/29

N2 - Oxytocin is a mammalian hormone that is released mainly after distension of the uterine cervix. In this study, we report that oxytocin stimulates intracellular release of calcium, and also activates AMPK (AMP-activated protein kinase) in C2C12 myoblast cells in a time/dose-dependent manner. Oxytocin receptor mRNA was detected in C2C12 cells. In addition, oxytocin stimulated glucose uptake and, moreover, inhibition of either CaMKK (Ca2+/calmodulin-dependent protein kinase kinase) or AMPK blocked oxytocin-mediated AMPK activation and glucose uptake. Taken together, our findings suggest that oxytocin may serve a peripheral metabolic function in skeletal muscle cells through the calcium-CaMKK-AMPK pathway.

AB - Oxytocin is a mammalian hormone that is released mainly after distension of the uterine cervix. In this study, we report that oxytocin stimulates intracellular release of calcium, and also activates AMPK (AMP-activated protein kinase) in C2C12 myoblast cells in a time/dose-dependent manner. Oxytocin receptor mRNA was detected in C2C12 cells. In addition, oxytocin stimulated glucose uptake and, moreover, inhibition of either CaMKK (Ca2+/calmodulin-dependent protein kinase kinase) or AMPK blocked oxytocin-mediated AMPK activation and glucose uptake. Taken together, our findings suggest that oxytocin may serve a peripheral metabolic function in skeletal muscle cells through the calcium-CaMKK-AMPK pathway.

KW - ACC

KW - AMPK

KW - Calcium

KW - CaMKK

KW - Oxytocin

UR - http://www.scopus.com/inward/record.url?scp=56549092664&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=56549092664&partnerID=8YFLogxK

U2 - 10.1016/j.regpep.2008.05.001

DO - 10.1016/j.regpep.2008.05.001

M3 - Article

VL - 151

SP - 71

EP - 74

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 1-3

ER -