p-Coumaric acid inhibition of CREB phosphorylation reduces cellular melanogenesis

Hee jin Jun, Ji Hae Lee, Bo Ram Cho, Woo Duck Seo, Dong Woo Kim, Kang Jin Cho, Sung-Joon Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)


We investigated the in vitro effects of p-coumaric acid on melanogenesis. The melanin content in B16F1 cells stimulated with p-coumaric acid significantly decreased (68 % vs. control) through inhibition of tyrosinase enzyme activity assessed using both cell-free and cell-based assays (46 and 27 % compared with control, respectively). In addition, stimulating B16F1 cells with p-coumaric acid reduced cyclic adenosine monophosphate (cAMP)-responsive element-binding protein (CREB) protein phosphorylation (26 % vs. control), which in turn downregulated the expression of the microphthalmia-associated transcription factor (MITF) and its target gene tyrosinase (27 and 20 % vs. control, respectively). p-Coumaric acid has a hypopigmentation effect in melanocytes by both directly inhibiting tyrosinase enzyme activity and reducing CREB phosphorylation, which inhibits MITF and tyrosinase expression.

Original languageEnglish
Pages (from-to)1207-1211
Number of pages5
JournalEuropean Food Research and Technology
Issue number6
Publication statusPublished - 2012 Dec 1



  • cAMP-responsive element-binding protein
  • Melanogenesis
  • Microphthalmia-associated transcription factor
  • p-Coumaric acid
  • Tyrosinase

ASJC Scopus subject areas

  • Food Science
  • Chemistry(all)
  • Biochemistry
  • Biotechnology
  • Industrial and Manufacturing Engineering

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