P19 Embryonal carcinoma cells

A new model for the study of endothelial cell differentiation

Seung Cheol Choi, Ji Hyun Choi, Wan Joo Shim, Do-Sun Lim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

P19 embryonal carcinoma cells were examined under different conditions of embryoid body (EB) formation to evaluate endothelial differentiation. A prominent capillary-like network including PECAM-1 positive cells and upregulated expression of endothelial markers Tie-1, Tie-2, Flk-1 and PECAM-1 were identified in the DMSO-treated group during EB formation as well as after VEGF treatment. About 4% of the P19 cells differentiated into PECAM-1 positive cells on day 14 of differentiation. These results suggest that the P19 cells have the potential for endothelial cell differentiation and provide a new model system for future research.

Original languageEnglish
Pages (from-to)1169-1175
Number of pages7
JournalBiotechnology Letters
Volume30
Issue number7
DOIs
Publication statusPublished - 2008 Jul 1

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CD31 Antigens
Embryonal Carcinoma Stem Cells
Endothelial cells
Cell Differentiation
Endothelial Cells
Cells
Embryoid Bodies
Dimethyl Sulfoxide
Vascular Endothelial Growth Factor A

Keywords

  • Carcinoma cells
  • Dimethyl sulphoxide
  • Embryoid body
  • Endothelial cell differentiation
  • P19 embryonal carcinoma cells
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

P19 Embryonal carcinoma cells : A new model for the study of endothelial cell differentiation. / Choi, Seung Cheol; Choi, Ji Hyun; Shim, Wan Joo; Lim, Do-Sun.

In: Biotechnology Letters, Vol. 30, No. 7, 01.07.2008, p. 1169-1175.

Research output: Contribution to journalArticle

Choi, Seung Cheol ; Choi, Ji Hyun ; Shim, Wan Joo ; Lim, Do-Sun. / P19 Embryonal carcinoma cells : A new model for the study of endothelial cell differentiation. In: Biotechnology Letters. 2008 ; Vol. 30, No. 7. pp. 1169-1175.
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