p38 mitogen-activated protein kinase mediates lipopolysaccharide, not interferon-γ, -induced inducible nitric oxide synthase expression in mouse BV2 microglial cells

Inn O. Han, Ki W. Kim, Jong Hoon Ryu, Won-Ki Kim

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The present study examined the role of mitogen-activated protein kinases (MAPKs) in inducible nitric oxide synthase (iNOS) induction by lipopolysaccharide (LPS) or interferon-γ (IFN-γ) in the murine microglial cell line BV2 cells. LPS rapidly (<2 h) induced iNOS mRNA expression whereas IFN-γ did not within 8 h after stimulation. LPS activated three MAPK subtypes, extracellular signal-regulated kinase (ERK), p38 and c-Jun N-terminal kinase (JNK) as early as 15 min after stimulation. In contrast, IFN-γ activated only ERK after 6 h of stimulation and did not alter the activity of p38 and JNK. LPS-induced iNOS expression was markedly decreased by the p38 inhibitor SB203580, but not by the MAPK or ERK kinase inhibitor PD98059. IFN-γ-induced nitric oxide (NO) generation was partially inhibited by PD98059 and SB203580 only in combination. The results demonstrate that MAPKs differentially mediate NO production by LPS- and IFN-γ in BV2 cells.

Original languageEnglish
Pages (from-to)9-12
Number of pages4
JournalNeuroscience Letters
Volume325
Issue number1
DOIs
Publication statusPublished - 2002 May 31
Externally publishedYes

Fingerprint

p38 Mitogen-Activated Protein Kinases
Nitric Oxide Synthase Type II
Interferons
Lipopolysaccharides
Mitogen-Activated Protein Kinases
JNK Mitogen-Activated Protein Kinases
Extracellular Signal-Regulated MAP Kinases
Mitogen-Activated Protein Kinase 12
Nitric Oxide
Phosphotransferases
Cell Line
Messenger RNA
SB 203580
2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one

Keywords

  • c-Jun N-terminal kinase
  • Extracellular signal-regulated kinase
  • Inducible nitric oxide synthase
  • Interferon-γ
  • Lipopolysaccharide
  • Microglia
  • Nitric oxide
  • p38

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

p38 mitogen-activated protein kinase mediates lipopolysaccharide, not interferon-γ, -induced inducible nitric oxide synthase expression in mouse BV2 microglial cells. / Han, Inn O.; Kim, Ki W.; Ryu, Jong Hoon; Kim, Won-Ki.

In: Neuroscience Letters, Vol. 325, No. 1, 31.05.2002, p. 9-12.

Research output: Contribution to journalArticle

@article{16d788d40b7741edb670c95a69cefa3f,
title = "p38 mitogen-activated protein kinase mediates lipopolysaccharide, not interferon-γ, -induced inducible nitric oxide synthase expression in mouse BV2 microglial cells",
abstract = "The present study examined the role of mitogen-activated protein kinases (MAPKs) in inducible nitric oxide synthase (iNOS) induction by lipopolysaccharide (LPS) or interferon-γ (IFN-γ) in the murine microglial cell line BV2 cells. LPS rapidly (<2 h) induced iNOS mRNA expression whereas IFN-γ did not within 8 h after stimulation. LPS activated three MAPK subtypes, extracellular signal-regulated kinase (ERK), p38 and c-Jun N-terminal kinase (JNK) as early as 15 min after stimulation. In contrast, IFN-γ activated only ERK after 6 h of stimulation and did not alter the activity of p38 and JNK. LPS-induced iNOS expression was markedly decreased by the p38 inhibitor SB203580, but not by the MAPK or ERK kinase inhibitor PD98059. IFN-γ-induced nitric oxide (NO) generation was partially inhibited by PD98059 and SB203580 only in combination. The results demonstrate that MAPKs differentially mediate NO production by LPS- and IFN-γ in BV2 cells.",
keywords = "c-Jun N-terminal kinase, Extracellular signal-regulated kinase, Inducible nitric oxide synthase, Interferon-γ, Lipopolysaccharide, Microglia, Nitric oxide, p38",
author = "Han, {Inn O.} and Kim, {Ki W.} and Ryu, {Jong Hoon} and Won-Ki Kim",
year = "2002",
month = "5",
day = "31",
doi = "10.1016/S0304-3940(02)00218-5",
language = "English",
volume = "325",
pages = "9--12",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - p38 mitogen-activated protein kinase mediates lipopolysaccharide, not interferon-γ, -induced inducible nitric oxide synthase expression in mouse BV2 microglial cells

AU - Han, Inn O.

AU - Kim, Ki W.

AU - Ryu, Jong Hoon

AU - Kim, Won-Ki

PY - 2002/5/31

Y1 - 2002/5/31

N2 - The present study examined the role of mitogen-activated protein kinases (MAPKs) in inducible nitric oxide synthase (iNOS) induction by lipopolysaccharide (LPS) or interferon-γ (IFN-γ) in the murine microglial cell line BV2 cells. LPS rapidly (<2 h) induced iNOS mRNA expression whereas IFN-γ did not within 8 h after stimulation. LPS activated three MAPK subtypes, extracellular signal-regulated kinase (ERK), p38 and c-Jun N-terminal kinase (JNK) as early as 15 min after stimulation. In contrast, IFN-γ activated only ERK after 6 h of stimulation and did not alter the activity of p38 and JNK. LPS-induced iNOS expression was markedly decreased by the p38 inhibitor SB203580, but not by the MAPK or ERK kinase inhibitor PD98059. IFN-γ-induced nitric oxide (NO) generation was partially inhibited by PD98059 and SB203580 only in combination. The results demonstrate that MAPKs differentially mediate NO production by LPS- and IFN-γ in BV2 cells.

AB - The present study examined the role of mitogen-activated protein kinases (MAPKs) in inducible nitric oxide synthase (iNOS) induction by lipopolysaccharide (LPS) or interferon-γ (IFN-γ) in the murine microglial cell line BV2 cells. LPS rapidly (<2 h) induced iNOS mRNA expression whereas IFN-γ did not within 8 h after stimulation. LPS activated three MAPK subtypes, extracellular signal-regulated kinase (ERK), p38 and c-Jun N-terminal kinase (JNK) as early as 15 min after stimulation. In contrast, IFN-γ activated only ERK after 6 h of stimulation and did not alter the activity of p38 and JNK. LPS-induced iNOS expression was markedly decreased by the p38 inhibitor SB203580, but not by the MAPK or ERK kinase inhibitor PD98059. IFN-γ-induced nitric oxide (NO) generation was partially inhibited by PD98059 and SB203580 only in combination. The results demonstrate that MAPKs differentially mediate NO production by LPS- and IFN-γ in BV2 cells.

KW - c-Jun N-terminal kinase

KW - Extracellular signal-regulated kinase

KW - Inducible nitric oxide synthase

KW - Interferon-γ

KW - Lipopolysaccharide

KW - Microglia

KW - Nitric oxide

KW - p38

UR - http://www.scopus.com/inward/record.url?scp=0037204864&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037204864&partnerID=8YFLogxK

U2 - 10.1016/S0304-3940(02)00218-5

DO - 10.1016/S0304-3940(02)00218-5

M3 - Article

VL - 325

SP - 9

EP - 12

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1

ER -