p67 isoform of mouse disabled 2 protein acts as a transcriptional activator during the differentiation of F9 cells

Young Cho Si Young Cho, Won Jeon Jae Won Jeon, Ho Lee Sang Ho Lee, S. S. Park

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

The mouse disabled 2(mDab2) gene is a mouse homologue of the Drosophila disabled gene and is alternatively spliced to form two isoforms, p96 and p67. Although p96 has been known to regulate the Ras-Sos G-protein signal transduction pathway by inter-acting with Grb2, little is known about the biological function of p67. Recent studies have shown that the expression of mDab2 is markedly up-regulated during the retinoic acid (RA)-induced differentiation of F9 cells, suggesting another role for mDab2 in cell differentiation [Cho, Lee and Park (1999) Mol. Cells 9, 179-184). In the present study, we first elucidated the biological function of p67 isoform of mDab2 and identified its binding partner. Unlike p96, p67 largely resides in RA-treated F9 cell nuclei. In this system, p67 interacts with mouse androgen-receptor interacting protein 3, termed the mDab2 interacting protein, which acts as a transcriptional co-regulator. By using a fusion protein with a heterologous DNA-binding domain (GAL4), we showed that p67 had an intrinsic transcriptional activation function. These results suggest that mDab2 p67 may function as a transcriptional co-factor for certain complexes of transcriptional regulatory dements involved in the RA-induced differentiation of F9 cells.

Original languageEnglish
Pages (from-to)645-650
Number of pages6
JournalBiochemical Journal
Volume352
Issue number3
DOIs
Publication statusPublished - 2000 Dec 15

Keywords

  • Proline-rich domain
  • Retinoic acid
  • Transactivation
  • mDab2 interacting protein

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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