Panax ginseng effects on DNA damage, CYP1A1 expression and histopathological changes in testes of rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin

Ju-Han Lee, Dong Geun Sul, Eunha Oh, Woon Won Jung, Kwang Woo Hwang, Tae Sung Hwang, Keon Cheol Lee, Nam Hee Won

Research output: Contribution to journalArticle

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Abstract

The effects of Panax ginseng extracts on DNA damage, expression of cytochrome P450 (CYP) 1A1 and reproductive toxicity were evaluated in the testis of rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxinthe (TCDD). Fifty rats were divided into five groups according to treatment with 2,3,7,8-TCDD and P. ginseng extracts. Single cell gel electrophoresis assays were performed to evaluate DNA damage that occurred in the lymphocytes of rats. Histological changes in the seminiferous tubules of the testis were determined using Johnsen's scoring system and Real Time-PCR was performed to evaluate the mRNA expression of CYP1A1. Significant pathological effects were observed in the 2,3,7,8-TCDD treated rats including a reduced seminiferous tubular diameter, an increased number of damaged tubules (maturation arrest, eosinophilic degeneration and spermatid giant cells) and increased Johnsen's score. DNA damage and the expression of CYP1A1 mRNA were significantly increased in rat testes. There were no significant differences between the control and animals treated with P. ginseng extracts. However, a significantly decreased level of DNA damage, decreased CYP1A1 expression and reduced pathological effects were observed in the 2,3,7,8-TCDD with P. ginseng extracts treated groups when compared with the TCDD treated group. In summary, our study demonstrates that 2,3,7,8-TCDD induces the pathological and genotoxical damage in rat testes, while P. ginseng extract treatment exhibits a therapeutic capacity to reduce these effects via reduction of CYP1A1 mRNA.

Original languageEnglish
Pages (from-to)2237-2244
Number of pages8
JournalFood and Chemical Toxicology
Volume45
Issue number11
DOIs
Publication statusPublished - 2007 Nov 1

Fingerprint

tetrachlorodibenzo-p-dioxin
Panax ginseng
Panax
Cytochrome P-450 CYP1A1
DNA damage
DNA Damage
Testis
Rats
testes
DNA
rats
extracts
Messenger RNA
reproductive toxicology
Seminiferous Tubules
Comet Assay
Spermatids
seminiferous tubules
Lymphocytes
spermatids

Keywords

  • 2,3,7,8-TCDD
  • CYP1A1
  • DNA damage
  • Panax ginseng
  • RT-PCR
  • Testes

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Panax ginseng effects on DNA damage, CYP1A1 expression and histopathological changes in testes of rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin. / Lee, Ju-Han; Sul, Dong Geun; Oh, Eunha; Jung, Woon Won; Hwang, Kwang Woo; Hwang, Tae Sung; Lee, Keon Cheol; Won, Nam Hee.

In: Food and Chemical Toxicology, Vol. 45, No. 11, 01.11.2007, p. 2237-2244.

Research output: Contribution to journalArticle

Lee, Ju-Han ; Sul, Dong Geun ; Oh, Eunha ; Jung, Woon Won ; Hwang, Kwang Woo ; Hwang, Tae Sung ; Lee, Keon Cheol ; Won, Nam Hee. / Panax ginseng effects on DNA damage, CYP1A1 expression and histopathological changes in testes of rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin. In: Food and Chemical Toxicology. 2007 ; Vol. 45, No. 11. pp. 2237-2244.
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abstract = "The effects of Panax ginseng extracts on DNA damage, expression of cytochrome P450 (CYP) 1A1 and reproductive toxicity were evaluated in the testis of rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxinthe (TCDD). Fifty rats were divided into five groups according to treatment with 2,3,7,8-TCDD and P. ginseng extracts. Single cell gel electrophoresis assays were performed to evaluate DNA damage that occurred in the lymphocytes of rats. Histological changes in the seminiferous tubules of the testis were determined using Johnsen's scoring system and Real Time-PCR was performed to evaluate the mRNA expression of CYP1A1. Significant pathological effects were observed in the 2,3,7,8-TCDD treated rats including a reduced seminiferous tubular diameter, an increased number of damaged tubules (maturation arrest, eosinophilic degeneration and spermatid giant cells) and increased Johnsen's score. DNA damage and the expression of CYP1A1 mRNA were significantly increased in rat testes. There were no significant differences between the control and animals treated with P. ginseng extracts. However, a significantly decreased level of DNA damage, decreased CYP1A1 expression and reduced pathological effects were observed in the 2,3,7,8-TCDD with P. ginseng extracts treated groups when compared with the TCDD treated group. In summary, our study demonstrates that 2,3,7,8-TCDD induces the pathological and genotoxical damage in rat testes, while P. ginseng extract treatment exhibits a therapeutic capacity to reduce these effects via reduction of CYP1A1 mRNA.",
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AU - Lee, Ju-Han

AU - Sul, Dong Geun

AU - Oh, Eunha

AU - Jung, Woon Won

AU - Hwang, Kwang Woo

AU - Hwang, Tae Sung

AU - Lee, Keon Cheol

AU - Won, Nam Hee

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