Partial male sterility induced in tobacco by overproduction of mRNA of sweet potato small subunit ADP-glucose pyrophosphorylase

Jung M. Bae, Chee H. Harn, Kyoung H. Tae, Mi C. Suh, Jeong Sheop Shin, Jang R. Liu

Research output: Contribution to journalArticle

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Abstract

Previously we generated transgenic tobacco plants overexpressing sweet potato ADP-glucose pyrophosphorylase (AGPase) small subunit cDNA under control of CaMV 35S promoter. In order to understand the role of small subunit AGPase in pollen development, these transgenic tobacco plants were studied. Nine out of ten transgenic tobacco plants produced more than 50% aborted pollen grains. The two transgenic lines T4 and T27 produced more than 75 % aborted pollen grains. Analysis of inheritance of the transgene inserts in T4 and T27 revealed that transgene co-segregated with the pollen abortion phenotype. Microscopic examination of aborted pollen grains showed that their cytoplasm did not contain starch granules, nuclei or any other organelles. Transcript levels of the transgene were very high in stamen tissues of transgenic plants. Transcript levels were correlated with the severity of the pollen abortion phenotype in transgenic plants, indicating that the pollen abortion phenotype probably resulted from overproduction of sweet potato small subunit AGPase mRNAs. Western blot analysis revealed that AGPase small subunit polypeptide levels were significantly reduced in stamen tissues of T4 and T27 compared with control plant. These results suggest that AGPase small subunit gene may play an important role in pollen development.

Original languageEnglish
Pages (from-to)1273-1279
Number of pages7
JournalJournal of Plant Physiology
Volume158
Issue number10
Publication statusPublished - 2001 Nov 7

Fingerprint

Glucose-1-Phosphate Adenylyltransferase
Ipomoea batatas
Male Infertility
male fertility
sweet potatoes
Pollen
Tobacco
tobacco
abortion (plants)
pollen
Genetically Modified Plants
Messenger RNA
genetically modified organisms
transgenes
stamens
phenotype
Transgenes
transgenic plants
Phenotype
starch granules

Keywords

  • ADP-glucose pyrophosphorylase
  • Ipomoea batatas cv. White star
  • Nicotiana tabacum
  • Pollen development
  • Starch

ASJC Scopus subject areas

  • Plant Science

Cite this

Partial male sterility induced in tobacco by overproduction of mRNA of sweet potato small subunit ADP-glucose pyrophosphorylase. / Bae, Jung M.; Harn, Chee H.; Tae, Kyoung H.; Suh, Mi C.; Shin, Jeong Sheop; Liu, Jang R.

In: Journal of Plant Physiology, Vol. 158, No. 10, 07.11.2001, p. 1273-1279.

Research output: Contribution to journalArticle

Bae, Jung M. ; Harn, Chee H. ; Tae, Kyoung H. ; Suh, Mi C. ; Shin, Jeong Sheop ; Liu, Jang R. / Partial male sterility induced in tobacco by overproduction of mRNA of sweet potato small subunit ADP-glucose pyrophosphorylase. In: Journal of Plant Physiology. 2001 ; Vol. 158, No. 10. pp. 1273-1279.
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