Partial purification of antioxidative peptides from gelatin Hydrolysates of Alaska pollock Surimi refiner discharge

Min Soo Heu, Chan Ho Park, Hyung Jun Kim, Jae W. Park, Jin Soo Kim

Research output: Contribution to journalArticle

Abstract

This study is conducted to partially purify an antioxidative peptide in a two-step gelatin hydrolysate from Alaska pollock surimi refiner discharge, which was obtained by sequential treatment with Pronase E and Flavourzyme. The two-step gelatin hydrolysate was fractionated using chromatographic methods. Based on the same protein concentration of each fraction, the antioxidative activities (85.1-95.4%) of positive fractions fractionated by ion-exchange chromatography were higher than those (27.2-87.8%) from gel filtration. Then, further purification of the positive fractions was performed. Among them, the partially purified A1C1L2G1 and A1C1L2G2 fractions showed 96.2% and 85.1% inhibition, respectively, of linoleic acid peroxidation. The A1C1L2G1 fraction was composed of 15 kinds of amino acids and the predominant amino acids were proline, glycine and alanine. The results obtained in this study suggested that the fraction partially purified through chromatographic methods from the two-step gelatin hydrolysate of Alaska pollock surimi refiner discharge could be useful as a supplementary source for improving health functionality.

Original languageEnglish
Pages (from-to)249-257
Number of pages9
JournalFisheries and Aquatic Sciences
Volume12
Issue number4
DOIs
Publication statusPublished - 2009 Dec

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Keywords

  • Fish gelatin
  • Gelatin hydrolysate
  • Seafood by-products
  • Surimi
  • Surimi refiner discharge

ASJC Scopus subject areas

  • Oceanography
  • Biochemistry
  • Aquatic Science
  • Molecular Biology

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