Abstract
A hot pepper (Capsicum annuum) cDNA clone encoding pathogenesis-related protein 10 (CaPR-10) was isolated by differential screening of a cDNA library prepared from pepper leaves inoculated with tobacco mosaic virus pathotype (TMV-P0). CaPR-10 transcripts were induced in the incompatible interaction with TMV-P0 or Xanthomonas campestris pv. vesicatoria (Xcv) but not induced in the compatible interaction. Characterization of enzymatic properties of CaPR-10 indicated that the recombinant protein exhibits a ribonucleolytic activity against TMV RNA, as well as against pepper total RNA, and shows its putative antiviral activity in several conditions. The CaPR-10 protein existed at very low level in leaf tissue but was dramatically induced as soon as plants were inoculated with TMV-P0, and this was correlated with the increase of its ribonucleolytic activity. Immunoblot analysis and pull-down assays using proteins extracted from pepper leaves showed that TMV-P0 inoculation led to the phosphorylation of CaPR-10, a modification that should affect its capacity for RNase function. We present data that the induction and subsequent phosphorylation of CaPR-10 increased its ribonucleolytic activity to cleave invading viral RNAs, and this activity should be important to its antiviral pathway during viral attack in vivo.
Original language | English |
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Pages (from-to) | 186-198 |
Number of pages | 13 |
Journal | Plant Journal |
Volume | 37 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2004 Jan |
Keywords
- Antiviral activity
- PR-10
- Pepper (Capsicum annuum L.)
- Ribonucleolytic activity
- Tobacco mosaic virus
ASJC Scopus subject areas
- Genetics
- Plant Science
- Cell Biology