Pathophysiological changes induced by Pseudomonas aeruginosa infection are involved in MMP-12 and MMP-13 upregulation in human carcinoma epithelial cells and a pneumonia mouse model

Ji Won Park, In Sik Shin, Un Hwan Ha, Sei Ryang Oh, Jae-Hong Kim, Kyung Seop Ahn

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Pseudomonas aeruginosa infections persist in patients with cystic fibrosis (CF) and drive lung disease progression. P. aeruginosa potently activates the innate immune system mostly through the recognition of pathogen-associated molecular patterns, such as flagellin. Matrix metalloproteinases 12 and 13 (MMP-12 and MMP-13, respectively) exacerbate chronic lung infection and inflammation by promoting uncontrolled tissue rearrangements and fibrosis, yet the underlying molecular mechanisms by which this occurs remain largely unknown. In this study, we used quantitative bacteriology, histological examination, and proinflammatory cytokine levels to evaluate the effects of MMP-12 and MMP-13 on P. aeruginosa strain K-induced infection and pneumonia in H292 epithelial cells and mice, respectively. Under inflammatory stimulation, mRNA and protein expression levels of proinflammatory mediators were higher in strain K-infected mice and cells than in uninfected counterparts, in which MMP-12 and MMP-13 expression reached levels similar to those observed in epithelial cells. Moreover, we also found that the NF-κB pathway might be involved in the induction of cytokines in response to strain K infection. Taken together, these data suggest that MMP-12 and MMP-13 alter strain K infection in mice and play a role in inflammatory regulation by modulating cytokine levels.

Original languageEnglish
Pages (from-to)4791-4799
Number of pages9
JournalInfection and Immunity
Volume83
Issue number12
DOIs
Publication statusPublished - 2015

Fingerprint

Pseudomonas Infections
Matrix Metalloproteinases
Pseudomonas aeruginosa
Pneumonia
Up-Regulation
Epithelial Cells
Carcinoma
Cytokines
Infection
Matrix Metalloproteinase 12
Matrix Metalloproteinase 13
Flagellin
Bacteriology
Cystic Fibrosis
Lung Diseases
Disease Progression
Immune System
Fibrosis
Messenger RNA

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Pathophysiological changes induced by Pseudomonas aeruginosa infection are involved in MMP-12 and MMP-13 upregulation in human carcinoma epithelial cells and a pneumonia mouse model. / Park, Ji Won; Shin, In Sik; Ha, Un Hwan; Oh, Sei Ryang; Kim, Jae-Hong; Ahn, Kyung Seop.

In: Infection and Immunity, Vol. 83, No. 12, 2015, p. 4791-4799.

Research output: Contribution to journalArticle

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