Patterning protein molecules on poly(ethylene glycol) coated Si(1 1 1)

Yongseok Jun; Taewoon Cha; Athena Guo; X. Y. Zhu

doi:10.1016/j.biomaterials.2003.10.053

Patterning protein molecules on poly(ethylene glycol) coated Si(1 1 1)

Yongseok Jun, Taewoon Cha, Athena Guo, X. Y. Zhu

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

We demonstrate spatially localized immobilization of protein molecules on high-density poly(ethylene glycol) (PEG) coated Si(111). Patterns of HO- and CH₃O-terminated PEG regions are formed on silicon surfaces based on soft lithography techniques and an efficient reaction between alcohol functional groups and chlorine-terminated silicon. Activation of the HO-terminated PEG brush is achieved via either partial oxidation to form aldehyde groups or via attachment of efficient leaving groups. Protein molecules are covalently immobilized to these activated regions on the PEG/Si surface.

Original language	English
Pages (from-to)	3503-3509
Number of pages	7
Journal	Biomaterials
Volume	25
Issue number	17
DOIs	https://doi.org/10.1016/j.biomaterials.2003.10.053
Publication status	Published - 2004 Aug
Externally published	Yes

Keywords

Polyethylene oxide
Protein adsorption
Surface grafting
Surface modification

ASJC Scopus subject areas

Bioengineering
Ceramics and Composites
Biophysics
Biomaterials
Mechanics of Materials

Access to Document

10.1016/j.biomaterials.2003.10.053

Cite this

@article{fe0b7e7066ee4f10adabb82816db2288,

title = "Patterning protein molecules on poly(ethylene glycol) coated Si(1 1 1)",

abstract = "We demonstrate spatially localized immobilization of protein molecules on high-density poly(ethylene glycol) (PEG) coated Si(111). Patterns of HO- and CH3O-terminated PEG regions are formed on silicon surfaces based on soft lithography techniques and an efficient reaction between alcohol functional groups and chlorine-terminated silicon. Activation of the HO-terminated PEG brush is achieved via either partial oxidation to form aldehyde groups or via attachment of efficient leaving groups. Protein molecules are covalently immobilized to these activated regions on the PEG/Si surface.",

keywords = "Polyethylene oxide, Protein adsorption, Surface grafting, Surface modification",

author = "Yongseok Jun and Taewoon Cha and Athena Guo and Zhu, {X. Y.}",

note = "Funding Information: Financial support from the National Science Foundation, Grant BES-0234005 to X.Y.Z., and National Institute of Health, Grant 1R43RR17130-01 to A.G., are gratefully acknowledged. We thank Prof. Younan Xia (University of Washington, Seattle) for generously providing the PDMS stamps. ",

year = "2004",

month = aug,

doi = "10.1016/j.biomaterials.2003.10.053",

language = "English",

volume = "25",

pages = "3503--3509",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier BV",

number = "17",

}

TY - JOUR

T1 - Patterning protein molecules on poly(ethylene glycol) coated Si(1 1 1)

AU - Jun, Yongseok

AU - Cha, Taewoon

AU - Guo, Athena

AU - Zhu, X. Y.

N1 - Funding Information: Financial support from the National Science Foundation, Grant BES-0234005 to X.Y.Z., and National Institute of Health, Grant 1R43RR17130-01 to A.G., are gratefully acknowledged. We thank Prof. Younan Xia (University of Washington, Seattle) for generously providing the PDMS stamps.

PY - 2004/8

Y1 - 2004/8

N2 - We demonstrate spatially localized immobilization of protein molecules on high-density poly(ethylene glycol) (PEG) coated Si(111). Patterns of HO- and CH3O-terminated PEG regions are formed on silicon surfaces based on soft lithography techniques and an efficient reaction between alcohol functional groups and chlorine-terminated silicon. Activation of the HO-terminated PEG brush is achieved via either partial oxidation to form aldehyde groups or via attachment of efficient leaving groups. Protein molecules are covalently immobilized to these activated regions on the PEG/Si surface.

AB - We demonstrate spatially localized immobilization of protein molecules on high-density poly(ethylene glycol) (PEG) coated Si(111). Patterns of HO- and CH3O-terminated PEG regions are formed on silicon surfaces based on soft lithography techniques and an efficient reaction between alcohol functional groups and chlorine-terminated silicon. Activation of the HO-terminated PEG brush is achieved via either partial oxidation to form aldehyde groups or via attachment of efficient leaving groups. Protein molecules are covalently immobilized to these activated regions on the PEG/Si surface.

KW - Polyethylene oxide

KW - Protein adsorption

KW - Surface grafting

KW - Surface modification

UR - http://www.scopus.com/inward/record.url?scp=1542328780&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2003.10.053

DO - 10.1016/j.biomaterials.2003.10.053

M3 - Article

C2 - 15020124

AN - SCOPUS:1542328780

SN - 0142-9612

VL - 25

SP - 3503

EP - 3509

JO - Biomaterials

JF - Biomaterials

IS - 17

ER -

Patterning protein molecules on poly(ethylene glycol) coated Si(1 1 1)

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this