Patterns of plasma fatty acids in rat models with adenovirus infection

Jeong Paik Man, Ho Park Ki, Joong Jean Park, Rae Kim Kyoung, Hwan Ahn Young, Tae Shin Gyu, Gwang Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Adenoviral vectors are among the most promising vectors available for human gene therapy. However, the use of recombinant adenoviral vectors, including replication-competent adenovirus (RCA), raises a variety of safety concerns in relation to the development of new therapies based on gene therapy. To examine how organic compounds change in rat plasma following the injection of adenovirus, β-galactosidase expressing recombinant adenovirus (designated rAdLacZ) or RCA, we investigated the content of fatty acids (FAs), which are important biochemical indicators in pathological conditions. Pattern recognition analysis on the level of FAs in rat plasma is described for the visual discrimination of adenovirus infection groups from normal controls. Plasma FAs from four control rats (normal group), and from four rats with rAdLacZ infection and six rats with RCA infection (the two abnormal groups), were examined by gas chromatography-mass spectrometry in selected ion monitoring modes as their tert-butyldimethylsilyl derivatives. In total, 20 FAs were positively detected and quantified. The results of the Student's t-test on the normal mean of two abnormal groups, the levels of three FAs (p < 0.05) from rAdLacZ group and eleven FAs (p < 0.05) from RCA group were significantly different. When star symbol plotting was applied to the group mean values of 20 FAs after normalization to the corresponding normal mean values, the resulting eicosagonal star patterns of the two infected groups were distorted into similar shapes, but were distinguishable from each other. Thus, these approaches will be useful for screening and monitoring of diagnostic markers for the effects of infection following the use of adenoviral vectors in gene therapy.

Original languageEnglish
Pages (from-to)119-124
Number of pages6
JournalJournal of Biochemistry and Molecular Biology
Volume40
Issue number1
Publication statusPublished - 2007 Jan 1

Fingerprint

Adenoviridae Infections
Rats
Fatty Acids
Adenoviridae
Plasmas
Gene therapy
Genetic Therapy
Stars
Rat control
Galactosidases
Monitoring
Infection
Organic compounds
Gas chromatography
Gas Chromatography-Mass Spectrometry
Pattern recognition
Mass spectrometry
Screening
Reference Values
Ions

Keywords

  • Adenovirus
  • Fatty acids
  • Gas chromatographymass spectrometry
  • Infection
  • Star symbol plot

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Man, J. P., Ki, H. P., Park, J. J., Kyoung, R. K., Young, H. A., Gyu, T. S., & Lee, G. (2007). Patterns of plasma fatty acids in rat models with adenovirus infection. Journal of Biochemistry and Molecular Biology, 40(1), 119-124.

Patterns of plasma fatty acids in rat models with adenovirus infection. / Man, Jeong Paik; Ki, Ho Park; Park, Joong Jean; Kyoung, Rae Kim; Young, Hwan Ahn; Gyu, Tae Shin; Lee, Gwang.

In: Journal of Biochemistry and Molecular Biology, Vol. 40, No. 1, 01.01.2007, p. 119-124.

Research output: Contribution to journalArticle

Man, JP, Ki, HP, Park, JJ, Kyoung, RK, Young, HA, Gyu, TS & Lee, G 2007, 'Patterns of plasma fatty acids in rat models with adenovirus infection', Journal of Biochemistry and Molecular Biology, vol. 40, no. 1, pp. 119-124.
Man, Jeong Paik ; Ki, Ho Park ; Park, Joong Jean ; Kyoung, Rae Kim ; Young, Hwan Ahn ; Gyu, Tae Shin ; Lee, Gwang. / Patterns of plasma fatty acids in rat models with adenovirus infection. In: Journal of Biochemistry and Molecular Biology. 2007 ; Vol. 40, No. 1. pp. 119-124.
@article{6935e2a7447f48e6bafaa6b6834b75ad,
title = "Patterns of plasma fatty acids in rat models with adenovirus infection",
abstract = "Adenoviral vectors are among the most promising vectors available for human gene therapy. However, the use of recombinant adenoviral vectors, including replication-competent adenovirus (RCA), raises a variety of safety concerns in relation to the development of new therapies based on gene therapy. To examine how organic compounds change in rat plasma following the injection of adenovirus, β-galactosidase expressing recombinant adenovirus (designated rAdLacZ) or RCA, we investigated the content of fatty acids (FAs), which are important biochemical indicators in pathological conditions. Pattern recognition analysis on the level of FAs in rat plasma is described for the visual discrimination of adenovirus infection groups from normal controls. Plasma FAs from four control rats (normal group), and from four rats with rAdLacZ infection and six rats with RCA infection (the two abnormal groups), were examined by gas chromatography-mass spectrometry in selected ion monitoring modes as their tert-butyldimethylsilyl derivatives. In total, 20 FAs were positively detected and quantified. The results of the Student's t-test on the normal mean of two abnormal groups, the levels of three FAs (p < 0.05) from rAdLacZ group and eleven FAs (p < 0.05) from RCA group were significantly different. When star symbol plotting was applied to the group mean values of 20 FAs after normalization to the corresponding normal mean values, the resulting eicosagonal star patterns of the two infected groups were distorted into similar shapes, but were distinguishable from each other. Thus, these approaches will be useful for screening and monitoring of diagnostic markers for the effects of infection following the use of adenoviral vectors in gene therapy.",
keywords = "Adenovirus, Fatty acids, Gas chromatographymass spectrometry, Infection, Star symbol plot",
author = "Man, {Jeong Paik} and Ki, {Ho Park} and Park, {Joong Jean} and Kyoung, {Rae Kim} and Young, {Hwan Ahn} and Gyu, {Tae Shin} and Gwang Lee",
year = "2007",
month = "1",
day = "1",
language = "English",
volume = "40",
pages = "119--124",
journal = "BMB Reports",
issn = "1976-6696",
publisher = "The Biochemical Society of the Republic of Korea",
number = "1",

}

TY - JOUR

T1 - Patterns of plasma fatty acids in rat models with adenovirus infection

AU - Man, Jeong Paik

AU - Ki, Ho Park

AU - Park, Joong Jean

AU - Kyoung, Rae Kim

AU - Young, Hwan Ahn

AU - Gyu, Tae Shin

AU - Lee, Gwang

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Adenoviral vectors are among the most promising vectors available for human gene therapy. However, the use of recombinant adenoviral vectors, including replication-competent adenovirus (RCA), raises a variety of safety concerns in relation to the development of new therapies based on gene therapy. To examine how organic compounds change in rat plasma following the injection of adenovirus, β-galactosidase expressing recombinant adenovirus (designated rAdLacZ) or RCA, we investigated the content of fatty acids (FAs), which are important biochemical indicators in pathological conditions. Pattern recognition analysis on the level of FAs in rat plasma is described for the visual discrimination of adenovirus infection groups from normal controls. Plasma FAs from four control rats (normal group), and from four rats with rAdLacZ infection and six rats with RCA infection (the two abnormal groups), were examined by gas chromatography-mass spectrometry in selected ion monitoring modes as their tert-butyldimethylsilyl derivatives. In total, 20 FAs were positively detected and quantified. The results of the Student's t-test on the normal mean of two abnormal groups, the levels of three FAs (p < 0.05) from rAdLacZ group and eleven FAs (p < 0.05) from RCA group were significantly different. When star symbol plotting was applied to the group mean values of 20 FAs after normalization to the corresponding normal mean values, the resulting eicosagonal star patterns of the two infected groups were distorted into similar shapes, but were distinguishable from each other. Thus, these approaches will be useful for screening and monitoring of diagnostic markers for the effects of infection following the use of adenoviral vectors in gene therapy.

AB - Adenoviral vectors are among the most promising vectors available for human gene therapy. However, the use of recombinant adenoviral vectors, including replication-competent adenovirus (RCA), raises a variety of safety concerns in relation to the development of new therapies based on gene therapy. To examine how organic compounds change in rat plasma following the injection of adenovirus, β-galactosidase expressing recombinant adenovirus (designated rAdLacZ) or RCA, we investigated the content of fatty acids (FAs), which are important biochemical indicators in pathological conditions. Pattern recognition analysis on the level of FAs in rat plasma is described for the visual discrimination of adenovirus infection groups from normal controls. Plasma FAs from four control rats (normal group), and from four rats with rAdLacZ infection and six rats with RCA infection (the two abnormal groups), were examined by gas chromatography-mass spectrometry in selected ion monitoring modes as their tert-butyldimethylsilyl derivatives. In total, 20 FAs were positively detected and quantified. The results of the Student's t-test on the normal mean of two abnormal groups, the levels of three FAs (p < 0.05) from rAdLacZ group and eleven FAs (p < 0.05) from RCA group were significantly different. When star symbol plotting was applied to the group mean values of 20 FAs after normalization to the corresponding normal mean values, the resulting eicosagonal star patterns of the two infected groups were distorted into similar shapes, but were distinguishable from each other. Thus, these approaches will be useful for screening and monitoring of diagnostic markers for the effects of infection following the use of adenoviral vectors in gene therapy.

KW - Adenovirus

KW - Fatty acids

KW - Gas chromatographymass spectrometry

KW - Infection

KW - Star symbol plot

UR - http://www.scopus.com/inward/record.url?scp=33846606113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846606113&partnerID=8YFLogxK

M3 - Article

C2 - 17244492

AN - SCOPUS:33846606113

VL - 40

SP - 119

EP - 124

JO - BMB Reports

JF - BMB Reports

SN - 1976-6696

IS - 1

ER -