Pause-and-stop: The effects of osmotic stress on cell proliferation during early leaf development in Arabidopsis and a role for ethylene signaling in cell cycle arrest

Aleksandra Skirycz, Hannes Claeys, Stefanie de Bodt, Akira Oikawa, Shoko Shinoda, Megan Andriankaja, Katrien Maleux, Nubia Barbosa Eloy, Frederik Coppens, Sang-Dong Yoo, Kazuki Saito, Dirk Inzé

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Abstract

Despite its relevance for agricultural production, environmental stress-induced growth inhibition, which is responsible for-significant yield reductions, is only poorly understood. Here, we investigated the molecular mechanisms underlying cell-cycle inhibition in young proliferating leaves of the model plant Arabidopsis thaliana when subjected to mild osmotic stress. A detailed cellular analysis demonstrated that as soon as osmotic stress is sensed, cell cycle progression rapidly arrests,-but cells are kept in a latent ambivalent state allowing a quick recovery (pause). Remarkably, cell cycle arrest coincides with-an increase in 1-aminocyclopropane-1-carboxylate levels and the activation of ethylene signaling. Our work showed that-ethylene acts on cell cycle progression via inhibition of cyclin-dependent kinase A activity independently of EIN3 transcriptional-control. When the stress persists, cells exit the mitotic cell cycle and initiate the differentiation process (stop).-This stop is reflected by early endore duplication onset, in a process independent of ethylene. Nonetheless, the potential to-partially recover the decreased cell numbers remains due to the activity of meristemoids. Together, these data present a-conceptual framework to understand how environmental stress reduces plant growth.

Original languageEnglish
Pages (from-to)1876-1888
Number of pages13
JournalPlant Cell
Volume23
Issue number5
DOIs
Publication statusPublished - 2011 May 1
Externally publishedYes

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ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Skirycz, A., Claeys, H., de Bodt, S., Oikawa, A., Shinoda, S., Andriankaja, M., Maleux, K., Eloy, N. B., Coppens, F., Yoo, S-D., Saito, K., & Inzé, D. (2011). Pause-and-stop: The effects of osmotic stress on cell proliferation during early leaf development in Arabidopsis and a role for ethylene signaling in cell cycle arrest. Plant Cell, 23(5), 1876-1888. https://doi.org/10.1105/tpc.111.084160