PDMS-based micro PCR chip with Parylene coating

Young Shik Shin, Keunchang Cho, Sun Hee Lim, Seok Chung, Sung J. Park, Chanil Chung, Dong Chul Han, Jun Keun Chang

Research output: Contribution to journalArticle

269 Citations (Scopus)

Abstract

We have developed a microchip for polymerase chain reaction (PCR) with polydimethylsiloxane (PDMS). PDMS has good characteristics: it is cheap, transparent, easy to fabricate and biocompatible. But in micro PCR, the porosity of PDMS causes several critical problems such as bubble formation, sample evaporation and protein adsorption. To solve those problems, we coated the micro PCR chips with Parylene film, which has low permeability to moisture and long-term stability. We investigated the influence of low thermal conductivity of PDMS and Parylene on the thermal characteristics of the PCR chips with numerical analysis. The thermal responses of micro PCR chips were compared for three materials: silicon, glass and PDMS. From the results, we identified appropriate thermal responses of the PDMS-based micro PCR chips by heating both the top and bottom sides. We could successfully amplify the angiotensin converting enzyme gene with as small a volume as 2 μl on the PDMS-based micro PCR chips without any additives.

Original languageEnglish
Pages (from-to)768-774
Number of pages7
JournalJournal of Micromechanics and Microengineering
Volume13
Issue number5
DOIs
Publication statusPublished - 2003 Sep 1
Externally publishedYes

Fingerprint

polymerase chain reaction
Polymerase chain reaction
Polydimethylsiloxane
chips
coatings
Coatings
angiotensins
Bubble formation
Peptidyl-Dipeptidase A
Silicon
parylene
baysilon
moisture
genes
numerical analysis
enzymes
Numerical analysis
Thermal conductivity
permeability
Evaporation

ASJC Scopus subject areas

  • Computational Mechanics
  • Mechanics of Materials
  • Materials Science(all)
  • Instrumentation

Cite this

Shin, Y. S., Cho, K., Lim, S. H., Chung, S., Park, S. J., Chung, C., ... Chang, J. K. (2003). PDMS-based micro PCR chip with Parylene coating. Journal of Micromechanics and Microengineering, 13(5), 768-774. https://doi.org/10.1088/0960-1317/13/5/332

PDMS-based micro PCR chip with Parylene coating. / Shin, Young Shik; Cho, Keunchang; Lim, Sun Hee; Chung, Seok; Park, Sung J.; Chung, Chanil; Han, Dong Chul; Chang, Jun Keun.

In: Journal of Micromechanics and Microengineering, Vol. 13, No. 5, 01.09.2003, p. 768-774.

Research output: Contribution to journalArticle

Shin, YS, Cho, K, Lim, SH, Chung, S, Park, SJ, Chung, C, Han, DC & Chang, JK 2003, 'PDMS-based micro PCR chip with Parylene coating', Journal of Micromechanics and Microengineering, vol. 13, no. 5, pp. 768-774. https://doi.org/10.1088/0960-1317/13/5/332
Shin, Young Shik ; Cho, Keunchang ; Lim, Sun Hee ; Chung, Seok ; Park, Sung J. ; Chung, Chanil ; Han, Dong Chul ; Chang, Jun Keun. / PDMS-based micro PCR chip with Parylene coating. In: Journal of Micromechanics and Microengineering. 2003 ; Vol. 13, No. 5. pp. 768-774.
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