PEGylated conjugated linoleic acid stimulation of apoptosis via a p53-mediated signaling pathway in MCF-7 breast cancer cells

Ji Hye Seo, Hyun-Seuk Moon, In Yong Kim, Ding Ding Guo, Hong Gu Lee, Yun Jaie Choi, Chong Su Cho

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The objective of this study was to investigate whether PEGylated conjugated linoleic acid (PCLA), as compared with conjugated linoleic acid (CLA) alone, displays anti-cancer properties in MCF-7 breast cancer cells. To generate PCLA, CLA was simply coupled to poly(ethylene glycol) (PEG) at the melting state of PEG without a solvent or a catalyst. The coupling reaction generated an ester linkage between the carboxyl group of CLA and hydroxyl one of PEG. The half-life of the generated PCLA was 52 h at pH 7.4 at 37 °C, indicating that PCLA potentially acts as a pro-drug. Apoptosis of MCF-7 breast cancer cells treated with PCLA showed a dose response to PCLA concentration during treatment. In addition, pro-apoptotic proteins such as Bax were up-regulated, whereas anti-apoptotic proteins, such as Bcl-2, were down-regulated by treatment with both CLA and PCLA. The tumor suppressor gene p53 was significantly up-regulated by treatment with increasing concentrations of PCLA, suggesting that PCLA-induced apoptosis is regulated by a p53-mediated signaling pathway. Overall, the anti-cancer effects of PCLA on MCF-7 breast cancer cells may have therapeutic significance.

Original languageEnglish
Pages (from-to)621-626
Number of pages6
JournalEuropean Journal of Pharmaceutics and Biopharmaceutics
Volume70
Issue number2
DOIs
Publication statusPublished - 2008 Oct 1
Externally publishedYes

Fingerprint

Conjugated Linoleic Acids
Apoptosis
Breast Neoplasms
Apoptosis Regulatory Proteins
Ethylene Glycol
Prodrugs
Tumor Suppressor Genes
Hydroxyl Radical
Freezing

Keywords

  • Anti-cancer
  • Conjugated linoleic acid (CLA)
  • Nanoparticle
  • PEGylation
  • Poly (ethylene glycol) (PEG)
  • Pro-drug

ASJC Scopus subject areas

  • Biotechnology
  • Pharmaceutical Science

Cite this

PEGylated conjugated linoleic acid stimulation of apoptosis via a p53-mediated signaling pathway in MCF-7 breast cancer cells. / Seo, Ji Hye; Moon, Hyun-Seuk; Kim, In Yong; Guo, Ding Ding; Lee, Hong Gu; Choi, Yun Jaie; Cho, Chong Su.

In: European Journal of Pharmaceutics and Biopharmaceutics, Vol. 70, No. 2, 01.10.2008, p. 621-626.

Research output: Contribution to journalArticle

Seo, Ji Hye ; Moon, Hyun-Seuk ; Kim, In Yong ; Guo, Ding Ding ; Lee, Hong Gu ; Choi, Yun Jaie ; Cho, Chong Su. / PEGylated conjugated linoleic acid stimulation of apoptosis via a p53-mediated signaling pathway in MCF-7 breast cancer cells. In: European Journal of Pharmaceutics and Biopharmaceutics. 2008 ; Vol. 70, No. 2. pp. 621-626.
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