Pendimethalin induces apoptosis in testicular cells via hampering ER-mitochondrial function and autophagy

Jiyeon Ham, Whasun Lim, Gwonhwa Song

Research output: Contribution to journalArticlepeer-review

Abstract

Pendimethalin (PDM) is a dinitroaniline crop pesticide that is extensively utilized worldwide. However, the reproductive toxicity and cellular mechanisms of PDM have not been identified. Therefore, we elucidated the adverse effects of PDM on the reproductive system using mouse testicular Leydig and Sertoli cells (TM3 and TM4 cells, respectively). Our results demonstrated that PDM suppressed the viability and proliferation of TM3 and TM4 cells. Additionally, PDM induced cytosolic calcium upregulation and permeabilization of mitochondrial membrane potential in both TM3 and TM4 cells. We also verified that PDM activates the endoplasmic reticulum (ER) stress pathway and autophagy. Furthermore, we confirmed that activation of ER stress and autophagy were blocked by 2-aminoethoxydiphenyl borate (2-APB) treatment. Finally, we confirmed PDM-induced cell cycle arrest and apoptosis in TM3 and TM4 cells. Thus, we first demonstrated that PDM impedes the survival of testis cells, and further, their function.

Original languageEnglish
Article number116835
JournalEnvironmental Pollution
Volume278
DOIs
Publication statusPublished - 2021 Jun 1

Keywords

  • Autophagy
  • ER stress
  • Herbicides
  • Inositol 1,4,5-triphosphate receptors
  • Pendimethalin

ASJC Scopus subject areas

  • Toxicology
  • Pollution
  • Health, Toxicology and Mutagenesis

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