Pep-1 peptide-conjugated elastic liposomal formulation of taxifolin glycoside for the treatment of atopic dermatitis in NC/Nga mice

Myung Joo Kang, Jae Yoon Eum, Sang Han Park, Mean Hyung Kang, Kwan Hee Park, Sun Eun Choi, Min Won Lee, Kyung Ho Kang, Chil Hwan Oh, Young Wook Choi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In order to develop topical preparations of taxifolin glycoside (TXG) for the treatment of atopic dermatitis (AD), formulations of Pep-1 peptide-conjugated elastic liposomes (Pep1-EL) were examined for their in vitro skin permeation profile and in vivo therapeutic efficacy. TXG-loaded Pep1-EL - a nanovesicle consisting of phosphatidylcholine, Tween 80, N-[4-(p-maleimidophenyl)butyryl]-phosphatidylethanolamine (MPB-PE), and Pep-1 peptide - is 130. nm in size, and has a zeta potential of 25. mV and a deformability index value of 60. Here, we examined the skin permeability of several topical preparations using a Franz diffusion cell mounted with depilated mouse skin and found that formulations of Pep1-EL exhibited superior absorption when compared to aqueous solution, EL or Pep-1 peptide-admixed EL formulations. Both transepidermal water loss and skin surface hydration were also measured using AD-induced NC/Nga mice, and the TXG-loaded Pep1-EL treatment group displayed a significantly expedited recovery in skin barrier function when compared to the controls treated with a TXG aqueous solution (p<0.05). AD-associated immune responses - including serum interleukine-4, immunoglobulin E, and interferon-gamma - were also regulated by topical application of TXG-loaded Pep1-EL. In conclusion, the novel Pep1-EL formulation of TXG shows substantial promise in the treatment of AD as a result of its desirable skin delivery-promoting capability.

Original languageEnglish
Pages (from-to)198-204
Number of pages7
JournalInternational Journal of Pharmaceutics
Volume402
Issue number1-2
DOIs
Publication statusPublished - 2010 Dec 15

Fingerprint

Atopic Dermatitis
Glycosides
Liposomes
Skin
Therapeutics
taxifolin
Pep-1 peptide
Polysorbates
Phosphatidylcholines
Immunoglobulin E
Interferon-gamma
Permeability
Water
Serum

Keywords

  • Atopic dermatitis
  • Elastic liposomes
  • NC/Nga mice
  • Pep-1 peptide
  • Taxifolin glycoside

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Pep-1 peptide-conjugated elastic liposomal formulation of taxifolin glycoside for the treatment of atopic dermatitis in NC/Nga mice. / Kang, Myung Joo; Eum, Jae Yoon; Park, Sang Han; Kang, Mean Hyung; Park, Kwan Hee; Choi, Sun Eun; Lee, Min Won; Kang, Kyung Ho; Oh, Chil Hwan; Choi, Young Wook.

In: International Journal of Pharmaceutics, Vol. 402, No. 1-2, 15.12.2010, p. 198-204.

Research output: Contribution to journalArticle

Kang, Myung Joo ; Eum, Jae Yoon ; Park, Sang Han ; Kang, Mean Hyung ; Park, Kwan Hee ; Choi, Sun Eun ; Lee, Min Won ; Kang, Kyung Ho ; Oh, Chil Hwan ; Choi, Young Wook. / Pep-1 peptide-conjugated elastic liposomal formulation of taxifolin glycoside for the treatment of atopic dermatitis in NC/Nga mice. In: International Journal of Pharmaceutics. 2010 ; Vol. 402, No. 1-2. pp. 198-204.
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abstract = "In order to develop topical preparations of taxifolin glycoside (TXG) for the treatment of atopic dermatitis (AD), formulations of Pep-1 peptide-conjugated elastic liposomes (Pep1-EL) were examined for their in vitro skin permeation profile and in vivo therapeutic efficacy. TXG-loaded Pep1-EL - a nanovesicle consisting of phosphatidylcholine, Tween 80, N-[4-(p-maleimidophenyl)butyryl]-phosphatidylethanolamine (MPB-PE), and Pep-1 peptide - is 130. nm in size, and has a zeta potential of 25. mV and a deformability index value of 60. Here, we examined the skin permeability of several topical preparations using a Franz diffusion cell mounted with depilated mouse skin and found that formulations of Pep1-EL exhibited superior absorption when compared to aqueous solution, EL or Pep-1 peptide-admixed EL formulations. Both transepidermal water loss and skin surface hydration were also measured using AD-induced NC/Nga mice, and the TXG-loaded Pep1-EL treatment group displayed a significantly expedited recovery in skin barrier function when compared to the controls treated with a TXG aqueous solution (p<0.05). AD-associated immune responses - including serum interleukine-4, immunoglobulin E, and interferon-gamma - were also regulated by topical application of TXG-loaded Pep1-EL. In conclusion, the novel Pep1-EL formulation of TXG shows substantial promise in the treatment of AD as a result of its desirable skin delivery-promoting capability.",
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AU - Park, Kwan Hee

AU - Choi, Sun Eun

AU - Lee, Min Won

AU - Kang, Kyung Ho

AU - Oh, Chil Hwan

AU - Choi, Young Wook

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AB - In order to develop topical preparations of taxifolin glycoside (TXG) for the treatment of atopic dermatitis (AD), formulations of Pep-1 peptide-conjugated elastic liposomes (Pep1-EL) were examined for their in vitro skin permeation profile and in vivo therapeutic efficacy. TXG-loaded Pep1-EL - a nanovesicle consisting of phosphatidylcholine, Tween 80, N-[4-(p-maleimidophenyl)butyryl]-phosphatidylethanolamine (MPB-PE), and Pep-1 peptide - is 130. nm in size, and has a zeta potential of 25. mV and a deformability index value of 60. Here, we examined the skin permeability of several topical preparations using a Franz diffusion cell mounted with depilated mouse skin and found that formulations of Pep1-EL exhibited superior absorption when compared to aqueous solution, EL or Pep-1 peptide-admixed EL formulations. Both transepidermal water loss and skin surface hydration were also measured using AD-induced NC/Nga mice, and the TXG-loaded Pep1-EL treatment group displayed a significantly expedited recovery in skin barrier function when compared to the controls treated with a TXG aqueous solution (p<0.05). AD-associated immune responses - including serum interleukine-4, immunoglobulin E, and interferon-gamma - were also regulated by topical application of TXG-loaded Pep1-EL. In conclusion, the novel Pep1-EL formulation of TXG shows substantial promise in the treatment of AD as a result of its desirable skin delivery-promoting capability.

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