Pep-1 peptide-conjugated elastic liposomal formulation of taxifolin glycoside for the treatment of atopic dermatitis in NC/Nga mice

Myung Joo Kang, Jae Yoon Eum, Sang Han Park, Mean Hyung Kang, Kwan Hee Park, Sun Eun Choi, Min Won Lee, Kyung Ho Kang, Chil Hwan Oh, Young Wook Choi

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

In order to develop topical preparations of taxifolin glycoside (TXG) for the treatment of atopic dermatitis (AD), formulations of Pep-1 peptide-conjugated elastic liposomes (Pep1-EL) were examined for their in vitro skin permeation profile and in vivo therapeutic efficacy. TXG-loaded Pep1-EL - a nanovesicle consisting of phosphatidylcholine, Tween 80, N-[4-(p-maleimidophenyl)butyryl]-phosphatidylethanolamine (MPB-PE), and Pep-1 peptide - is 130. nm in size, and has a zeta potential of 25. mV and a deformability index value of 60. Here, we examined the skin permeability of several topical preparations using a Franz diffusion cell mounted with depilated mouse skin and found that formulations of Pep1-EL exhibited superior absorption when compared to aqueous solution, EL or Pep-1 peptide-admixed EL formulations. Both transepidermal water loss and skin surface hydration were also measured using AD-induced NC/Nga mice, and the TXG-loaded Pep1-EL treatment group displayed a significantly expedited recovery in skin barrier function when compared to the controls treated with a TXG aqueous solution (p<0.05). AD-associated immune responses - including serum interleukine-4, immunoglobulin E, and interferon-gamma - were also regulated by topical application of TXG-loaded Pep1-EL. In conclusion, the novel Pep1-EL formulation of TXG shows substantial promise in the treatment of AD as a result of its desirable skin delivery-promoting capability.

Original languageEnglish
Pages (from-to)198-204
Number of pages7
JournalInternational Journal of Pharmaceutics
Volume402
Issue number1-2
DOIs
Publication statusPublished - 2010 Dec 15

Keywords

  • Atopic dermatitis
  • Elastic liposomes
  • NC/Nga mice
  • Pep-1 peptide
  • Taxifolin glycoside

ASJC Scopus subject areas

  • Pharmaceutical Science

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