Peptide based protein kinase detection using a nanomechanical dynamic microcantilever

Kyo Seon Hwang, Jeong Hoon Lee, Dong Won Chun, Byung Hak Cha, Ga Young Han, Jung ho Park, Eun Gyeong Yang, Dae Sung Yoon, Tae Song Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A nanomechanical microcantilever promises to bring about revolution in the field of chemical and biological analysis system development. We report the peptide immobilized nanomechanical microcantilever operated in dynamic vibration mode for quantitative label-free detection of protein kinase A. The detection principle of dynamic cantilever is that specific interaction on the microcantilever surface lead to the dynamic response change of microcantilevers. We achieved the protein kinase A detection in the low concentration as much as 6.6 pM through measuring the dynamic response change of microcantilever.

Original languageEnglish
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherJapan Academic Association Inc
Pages768-770
Number of pages3
Publication statusPublished - 2006 Jan 1
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: 2006 Nov 52006 Nov 9

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period06/11/506/11/9

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Cyclic AMP-Dependent Protein Kinases
Protein Kinases
Peptides
Dynamic response
Proteins
Labels

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Hwang, K. S., Lee, J. H., Chun, D. W., Cha, B. H., Han, G. Y., Park, J. H., ... Kim, T. S. (2006). Peptide based protein kinase detection using a nanomechanical dynamic microcantilever. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 768-770). Japan Academic Association Inc.

Peptide based protein kinase detection using a nanomechanical dynamic microcantilever. / Hwang, Kyo Seon; Lee, Jeong Hoon; Chun, Dong Won; Cha, Byung Hak; Han, Ga Young; Park, Jung ho; Yang, Eun Gyeong; Yoon, Dae Sung; Kim, Tae Song.

Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. p. 768-770.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hwang, KS, Lee, JH, Chun, DW, Cha, BH, Han, GY, Park, JH, Yang, EG, Yoon, DS & Kim, TS 2006, Peptide based protein kinase detection using a nanomechanical dynamic microcantilever. in Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, pp. 768-770, 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan, 06/11/5.
Hwang KS, Lee JH, Chun DW, Cha BH, Han GY, Park JH et al. Peptide based protein kinase detection using a nanomechanical dynamic microcantilever. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc. 2006. p. 768-770
Hwang, Kyo Seon ; Lee, Jeong Hoon ; Chun, Dong Won ; Cha, Byung Hak ; Han, Ga Young ; Park, Jung ho ; Yang, Eun Gyeong ; Yoon, Dae Sung ; Kim, Tae Song. / Peptide based protein kinase detection using a nanomechanical dynamic microcantilever. Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. pp. 768-770
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