Performance evaluation of four rapid antigen tests for the detection of Respiratory syncytial virus

Bo Kyeung Jung, Sung Hyuk Choi, Jong Han Lee, Jung Hwa Lee, Chae Seung Lim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Rapid identification of Respiratory syncytial virus (RSV) is important in the management of infected patients. Rapid diagnostic tests (RDT) are widely used for this purpose. This study aimed to evaluate the clinical performance of four RSV antigen tests including the BinaxNow RSV Card test, SD Bioline RSV test, BD Veritor RSV test, and Humasis RSV antigen test in comparison with real-time RT–PCR as the reference method. Nasopharyngeal swabs were collected from 280 patients with symptoms of lower respiratory tract infection and stored at −80°C. All swabs were tested for RSV using four rapid antigen tests and real time RT–PCR. The sensitivity of the BinaxNow RSV Card test, SD Bioline RSV test, BD Veritor RSV test, and Humasis RSV Antigen tests were 62.5%, 61.3%, 65.0%, and 67.5% for RSV A, and 61.3%, 65.0%, 61.3%, and 67.5% for RSV B compared to real time RT–PCR, respectively. The specificity of BD Veritor RSV test was 95.8% and those of the other three RDTs was 100%. Commercial RSV antigen detection assays are useful tools for the rapid diagnosis of RSV infection. However, confirmatory testing is always recommended. J. Med. Virol. 88:1720–1724, 2016.

Original languageEnglish
Pages (from-to)1720-1724
Number of pages5
JournalJournal of Medical Virology
Volume88
Issue number10
DOIs
Publication statusPublished - 2016 Oct 1

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Respiratory Syncytial Viruses
Antigens
Respiratory Syncytial Virus Infections
Routine Diagnostic Tests
Respiratory Tract Infections

Keywords

  • rapid antigen test
  • Respiratory syncytial virus
  • RT–PCR
  • sensitivity
  • specificity

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Performance evaluation of four rapid antigen tests for the detection of Respiratory syncytial virus. / Jung, Bo Kyeung; Choi, Sung Hyuk; Lee, Jong Han; Lee, Jung Hwa; Lim, Chae Seung.

In: Journal of Medical Virology, Vol. 88, No. 10, 01.10.2016, p. 1720-1724.

Research output: Contribution to journalArticle

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