Performance of β-galactosidase pretreated with lactose to prevent activity loss during the enzyme immobilisation process

Yoon Seok Song, Jong H. Lee, Seong Woo Kang, Seung Wook Kim

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In this study, Kluyveromyces lactis β-galactosidase was pretreated with lactose to prevent loss of activity during the immobilisation process, and glutaraldehyde was used as a linker to immobilise β-galactosidase on the surface of a silica gel. The pretreatment of β-galactosidase strongly improved its activity after immobilisation. Specifically, the activity of pretreated immobilised β-galactosidase was 2.6 times greater than that of non-pretreated immobilised β-galactosidase. The optimal temperature, pH and ionic strength of buffer for pretreated immobilised β-galactosidase were 37 °C, pH 7.5 and 20 mM potassium phosphate buffer, respectively. These values were shifted by 5 °C and pH by 0.5 when compared to the soluble β-galactosidase. Moreover, the pretreated immobilised β-galactosidase showed a better reusability than did non-pretreated immobilised β-galactosidase, with 63.9% of its original activity being retained after 10 reuses.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalFood Chemistry
Volume123
Issue number1
DOIs
Publication statusPublished - 2010 Nov 1

Fingerprint

Galactosidases
Enzyme immobilization
galactosidases
immobilized enzymes
Lactose
Immobilization
lactose
Enzymes
Buffers
buffers
Kluyveromyces
Kluyveromyces marxianus var. lactis
potassium phosphates
Silica Gel
glutaraldehyde
Reusability
Glutaral
silica gel
ionic strength
Ionic strength

Keywords

  • β-Galactosidase
  • Immobilisation
  • Lactose
  • Pretreatment
  • Silica gel

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Performance of β-galactosidase pretreated with lactose to prevent activity loss during the enzyme immobilisation process. / Song, Yoon Seok; Lee, Jong H.; Kang, Seong Woo; Kim, Seung Wook.

In: Food Chemistry, Vol. 123, No. 1, 01.11.2010, p. 1-5.

Research output: Contribution to journalArticle

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