Peripheral mGluR5 antagonist attenuated craniofacial muscle pain and inflammation but not mGluR1 antagonist in lightly anesthetized rats

Ho Jeong Lee, Hyo Soon Choi, Jin Sook Ju, Yong Chul Bae, Sung Kyo Kim, Young Wook Yoon, Dong Kuk Ahn

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The present study investigated the role of peripheral group I metabotropic glutamate receptors (mGluRs) in MO-induced nociceptive behaviour and inflammation in the masseter muscles of lightly anesthetized rats. Experiments were carried out on male Sprague-Dawley rats weighing 300-400 g. After initial anesthesia with sodium pentobarbital (40 mg/kg, i.p.), one femoral vein was cannulated and connected to an infusion pump for intravenous infusion of sodium pentobarbital. The rate of infusion was adjusted to provide a constant level of anesthesia. Mustard oil (MO, 30 μl) was injected into the mid-region of the left masseter muscle via a 30-gauge needle over 10 s. After 30 μl injection of 5, 10, 15, or 20% MO into the masseter muscle, the total number of hindpaw shaking behaviour and extravasated Evans' blue dye concentration in the masseter muscle were significantly higher in the MO-treated group in a dose-dependent manner compared with the vehicle (mineral oil)-treated group. Intramuscular pretreatment with 3 or 5% lidocaine reduced MO-induced hindpaw shaking behaviour and increases in extravasated Evans' blue dye concentration. Intramuscular pretreatment with 5 mM MCPG, non-selective group I/II mGluR antagonist, or MPEP, a selective group I mGluR5 antagonist, produced a significant attenuation of MO-induced hindpaw shaking behaviour and increases in extravasated Evans' blue dye concentration in the masseter muscle while LY367385, a selective group I mGluR1 antagonist, did not affect MO-induced nociceptive behaviour and inflammation in the masseter muscle. These results indicate that peripheral mGluR5 plays important role in mediating MO-induced nociceptive behaviour and inflammation in the craniofacial muscle.

Original languageEnglish
Pages (from-to)378-385
Number of pages8
JournalBrain Research Bulletin
Volume70
Issue number4-6
DOIs
Publication statusPublished - 2006 Oct 16

Fingerprint

Masseter Muscle
Facial Pain
Myalgia
Inflammation
Evans Blue
Coloring Agents
Pentobarbital
alpha-methyl-4-carboxyphenylglycine
Anesthesia
Infusion Pumps
Mineral Oil
Metabotropic Glutamate Receptors
Femoral Vein
Lidocaine
Intravenous Infusions
Needles
Sprague Dawley Rats
metabotropic glutamate receptor type 1
Muscles
Injections

Keywords

  • Antinociception
  • Muscle inflammation
  • Muscle pain
  • Nociceptive behaviour
  • Peripheral mGluR

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Peripheral mGluR5 antagonist attenuated craniofacial muscle pain and inflammation but not mGluR1 antagonist in lightly anesthetized rats. / Lee, Ho Jeong; Choi, Hyo Soon; Ju, Jin Sook; Bae, Yong Chul; Kim, Sung Kyo; Yoon, Young Wook; Ahn, Dong Kuk.

In: Brain Research Bulletin, Vol. 70, No. 4-6, 16.10.2006, p. 378-385.

Research output: Contribution to journalArticle

Lee, Ho Jeong ; Choi, Hyo Soon ; Ju, Jin Sook ; Bae, Yong Chul ; Kim, Sung Kyo ; Yoon, Young Wook ; Ahn, Dong Kuk. / Peripheral mGluR5 antagonist attenuated craniofacial muscle pain and inflammation but not mGluR1 antagonist in lightly anesthetized rats. In: Brain Research Bulletin. 2006 ; Vol. 70, No. 4-6. pp. 378-385.
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