Peritoneal cells mediate immune responses and cross-protection against influenza A virus

Avishekh Gautam, Byoung Kwon Park, Te Ha Kim, Madhav Akauliya, Dongbum Kim, Sony Maharjan, Joongwon Park, Jinsoo Kim, Hanseul Lee, Man-Seong Park, Younghee Lee, Hyung Joo Kwon

Research output: Contribution to journalArticle

Abstract

Intraperitoneal inoculation with live influenza A virus confers protection against intranasal infections in mice and ferrets. However, the responses of peritoneal cells to influenza A virus have not been investigated. Here we show that intraperitoneal inoculation with A/WSN/1933 (H1N1) virus induced virus-reactive IgG production in the peritoneal cavity in mice. The infection resulted in substantial but transient B cell and macrophage depletion along with massive neutrophil infiltration, but virus growth was not detected. Influenza A viruses bound to α-2,6-linked sialic acids of B cells and macrophages and induced apoptotic death of peritoneal cavity cells. However, reinfection with A/WSN/1933 virus did not have adverse effects on immune cells most likely because of the neutralizing antibodies produced in response to the first exposure. Infection of BALB/c mice with A/WSN/1933 induced cross-protection against an otherwise lethal intraperitoneal dose of A/Hongkong/4801/2014 (H3N2) virus. This information suggests that immunological responses in the peritoneal cavity can induce effective defense against future virus infection. Considering the unexpected potent immunoregulatory activity of the peritoneal cells against influenza viruses, we suggest that comparative studies on various immune reactions after infection through different routes may contribute to better selection of vaccination routes in development of efficacious influenza vaccines.

Original languageEnglish
Article number1160
JournalFrontiers in immunology
Volume10
Issue numberMAY
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Cross Protection
Influenza A virus
Peritoneal Cavity
Viruses
Infection
B-Lymphocytes
Macrophages
H3N2 Subtype Influenza A Virus
Sialic Acids
H1N1 Subtype Influenza A Virus
Ferrets
Neutrophil Infiltration
Influenza Vaccines
Hong Kong
Virus Diseases
Neutralizing Antibodies
Orthomyxoviridae
Vaccination
Immunoglobulin G
Growth

Keywords

  • Apoptosis
  • Cross protection
  • Influenza A virus
  • Neutralizing antibody
  • Peritoneal cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Gautam, A., Park, B. K., Kim, T. H., Akauliya, M., Kim, D., Maharjan, S., ... Kwon, H. J. (2019). Peritoneal cells mediate immune responses and cross-protection against influenza A virus. Frontiers in immunology, 10(MAY), [1160]. https://doi.org/10.3389/fimmu.2019.01160

Peritoneal cells mediate immune responses and cross-protection against influenza A virus. / Gautam, Avishekh; Park, Byoung Kwon; Kim, Te Ha; Akauliya, Madhav; Kim, Dongbum; Maharjan, Sony; Park, Joongwon; Kim, Jinsoo; Lee, Hanseul; Park, Man-Seong; Lee, Younghee; Kwon, Hyung Joo.

In: Frontiers in immunology, Vol. 10, No. MAY, 1160, 01.01.2019.

Research output: Contribution to journalArticle

Gautam, A, Park, BK, Kim, TH, Akauliya, M, Kim, D, Maharjan, S, Park, J, Kim, J, Lee, H, Park, M-S, Lee, Y & Kwon, HJ 2019, 'Peritoneal cells mediate immune responses and cross-protection against influenza A virus', Frontiers in immunology, vol. 10, no. MAY, 1160. https://doi.org/10.3389/fimmu.2019.01160
Gautam, Avishekh ; Park, Byoung Kwon ; Kim, Te Ha ; Akauliya, Madhav ; Kim, Dongbum ; Maharjan, Sony ; Park, Joongwon ; Kim, Jinsoo ; Lee, Hanseul ; Park, Man-Seong ; Lee, Younghee ; Kwon, Hyung Joo. / Peritoneal cells mediate immune responses and cross-protection against influenza A virus. In: Frontiers in immunology. 2019 ; Vol. 10, No. MAY.
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