TY - JOUR
T1 - Permselective glucose sensing with GLUT1-rich cancer cell membranes
AU - Kim, Insu
AU - Kwon, Dohyung
AU - Lee, Dongtak
AU - Lee, Gyudo
AU - Yoon, Dae Sung
N1 - Funding Information:
This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (MSIP) (No. NRF-2016R1A2B4010269, NRF-2017R1A6A3A11034311 and NRF-2018M3C1B7020722). This material is also supported by the Ministry of Trade, Industry and Energy under Industrial Technology Innovation Program (No.10079316). Dr. Gyudo Lee is thankful for the financial support by the Korea University Grant.
PY - 2019/6/15
Y1 - 2019/6/15
N2 - Enzymatic blood glucose detection with selectivity is one of the most important conundrums, because human blood contains many components that can hinder enzyme-substrate reactions. Meanwhile, cancer cells express much higher levels of glucose transporter-1 on their cell membrane to selectively and excessively uptake more α-D-glucose than do normal cells. Inspired by such cellular permselectivity for glucose, herein we significantly improved the selectivity of a glucose sensor by using a breast cancer cell membrane (BCCM). The BCCM was extracted from MDA-MB-231 cells and coated onto an enzyme-deposited electrode via a vesicle fusion method. We investigated BCCM-coated sensors using ATR-FTIR, SEM, AFM, and cyclic voltammetry. The exceptional permselectivity of BCCM-coated sensors was validated using glucose solutions containing various interfering molecules (e.g., D-(−)-fructose, D-(+)-xylose, D-(+)-maltose, L-cysteine, L-ascorbic acid, and uric acid) and human serum (4.35–7.35 mM of glucose), implying their high potential for practical use.
AB - Enzymatic blood glucose detection with selectivity is one of the most important conundrums, because human blood contains many components that can hinder enzyme-substrate reactions. Meanwhile, cancer cells express much higher levels of glucose transporter-1 on their cell membrane to selectively and excessively uptake more α-D-glucose than do normal cells. Inspired by such cellular permselectivity for glucose, herein we significantly improved the selectivity of a glucose sensor by using a breast cancer cell membrane (BCCM). The BCCM was extracted from MDA-MB-231 cells and coated onto an enzyme-deposited electrode via a vesicle fusion method. We investigated BCCM-coated sensors using ATR-FTIR, SEM, AFM, and cyclic voltammetry. The exceptional permselectivity of BCCM-coated sensors was validated using glucose solutions containing various interfering molecules (e.g., D-(−)-fructose, D-(+)-xylose, D-(+)-maltose, L-cysteine, L-ascorbic acid, and uric acid) and human serum (4.35–7.35 mM of glucose), implying their high potential for practical use.
KW - Cancer cell membrane
KW - Electrochemical analysis
KW - Glucose biosensor
KW - Glucose transporter-1
KW - Permselectivity
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U2 - 10.1016/j.bios.2019.04.007
DO - 10.1016/j.bios.2019.04.007
M3 - Article
C2 - 31004924
AN - SCOPUS:85064445152
VL - 135
SP - 82
EP - 87
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
ER -