Permselective glucose sensing with GLUT1-rich cancer cell membranes

Insu Kim, Dohyung Kwon, Dongtak Lee, Gyudo Lee, Dae Sung Yoon

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Enzymatic blood glucose detection with selectivity is one of the most important conundrums, because human blood contains many components that can hinder enzyme-substrate reactions. Meanwhile, cancer cells express much higher levels of glucose transporter-1 on their cell membrane to selectively and excessively uptake more α-D-glucose than do normal cells. Inspired by such cellular permselectivity for glucose, herein we significantly improved the selectivity of a glucose sensor by using a breast cancer cell membrane (BCCM). The BCCM was extracted from MDA-MB-231 cells and coated onto an enzyme-deposited electrode via a vesicle fusion method. We investigated BCCM-coated sensors using ATR-FTIR, SEM, AFM, and cyclic voltammetry. The exceptional permselectivity of BCCM-coated sensors was validated using glucose solutions containing various interfering molecules (e.g., D-(−)-fructose, D-(+)-xylose, D-(+)-maltose, L-cysteine, L-ascorbic acid, and uric acid) and human serum (4.35–7.35 mM of glucose), implying their high potential for practical use.

Original languageEnglish
Pages (from-to)82-87
Number of pages6
JournalBiosensors and Bioelectronics
Volume135
DOIs
Publication statusPublished - 2019 Jun 15

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Keywords

  • Cancer cell membrane
  • Electrochemical analysis
  • Glucose biosensor
  • Glucose transporter-1
  • Permselectivity

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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