Peroral immunization of microencapsulated human VP8 * in combination with cholera toxin induces intestinal antibody responses

Doo Kyung Kang, Pyeung Hyeun Kim, Eun Ju Ko, Jun Young Seo, Seung Yong Seong, Yong Hee Kim, Ick Chan Kwon, Seo Young Jeong, Jai Myung Yang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To develop an orally delivered subunit vaccine for rotavirus infection, a trypsin cleavage product of VP4, recombinant VP8*, was expressed in Escherichia coli. The recombinant VP8* (rVP8*), purified by affinity chromatography, was reactive against human rotavirus positive serum in Western-blot analysis. To further evaluate the immunogenicity of the oral-delivered rVP8*, it was encapsulated with alginate-microshpere and administered in combination with cholera toxin (CT) as a mucosal adjuvant perorally into mice. The ELISPOT assay showed that the number of rVP8*-specific IgG1 antibody secreting cells increased about 3-fold and about 2-fold in spleen and Peyer's patch, respectively as compared to non-immune mice. In addition, the number of rVP8*-specific IgA antibody secreting cells increased about 2-fold in Peyer's patch. Finally, rVP8*-specific IgA antibody response was significantly enhanced in the intestinal fluids from the mice immunized perorally with encapsulated rVP8* and CT. Taken together, these results indicate that rVP8* possessed proper immunogenicity and it would be potentially useful as a subunit vaccine against rotavirus-associated disease through peroral immunization.

Original languageEnglish
Pages (from-to)609-616
Number of pages8
JournalMolecules and cells
Volume9
Issue number6
Publication statusPublished - 1999 Dec 31

Keywords

  • IgA
  • Microspheres
  • Rotavirus
  • Subunit vaccine
  • VP8*

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Kang, D. K., Kim, P. H., Ko, E. J., Seo, J. Y., Seong, S. Y., Kim, Y. H., Kwon, I. C., Jeong, S. Y., & Yang, J. M. (1999). Peroral immunization of microencapsulated human VP8 * in combination with cholera toxin induces intestinal antibody responses. Molecules and cells, 9(6), 609-616.