Peroxiredoxin I is an indicator of microglia activation and protects against hydrogen peroxide-mediated microglial death

Sun Uk Kim, Chang Nam Hwang, Hu Nan Sun, Mei Hua Jin, Ying Hao Han, Hwang Lee, Jin Man Kim, Sang Keun Kim, Dae Yeul Yu, Dong Seok Lee, Sang Ho Lee

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Imbalance between oxidative stress and antioxidative defence system is generally known as one of mechanisms causing an oxidative stress-medieated neuropathogenesis. Peroxiredoxins (Prxs), a family of antioxidative enzymes neutralizing cellular hydroperoxides, was characterized recently, but their distributions and roles have not been resolved clearly or controversial in the central nervous system, Therefore, the present study was carried out to determine the specific cell types that express Prx I in the mouse brain and primary neural cells, and to examine its antioxidative role in the preferential cell types. Immunohistochemical reactivity for Prx I was detected dominantly in oligodendrocytes and rarely in microglia, whereas strong and specific immunoreactivity for Prx I was observed exclusively in microglia of primary neural cell culture. Further evidences for Prx I specificity were its relatively high expression in BV-2 microglial cells and its upregulated expression in microglia after lipopolysaccharide (LPS) stimulation. These results imply that Prx I can be used as an indicator of microglial activation. Inhibition of p38 MAPK ablated LPS-mediated Prx I upregulation and sensitized the microglia to H2O2-mediated cell death. These findings indicate that Prx I function as a scavenger for H2O2 generated during microglial activation. The results of this study will help in unraveling the neuropathologic roles of the six Prx isoforms in neural function.

Original languageEnglish
Pages (from-to)820-825
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume31
Issue number5
DOIs
Publication statusPublished - 2008 May 1

Fingerprint

Peroxiredoxins
Microglia
Hydrogen Peroxide
Lipopolysaccharides
Oxidative Stress
Primary Cell Culture
Oligodendroglia
p38 Mitogen-Activated Protein Kinases
Protein Isoforms
Cell Death
Up-Regulation
Central Nervous System
Brain
Enzymes

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Peroxiredoxin I is an indicator of microglia activation and protects against hydrogen peroxide-mediated microglial death. / Kim, Sun Uk; Hwang, Chang Nam; Sun, Hu Nan; Jin, Mei Hua; Han, Ying Hao; Lee, Hwang; Kim, Jin Man; Kim, Sang Keun; Yu, Dae Yeul; Lee, Dong Seok; Lee, Sang Ho.

In: Biological and Pharmaceutical Bulletin, Vol. 31, No. 5, 01.05.2008, p. 820-825.

Research output: Contribution to journalArticle

Kim, Sun Uk ; Hwang, Chang Nam ; Sun, Hu Nan ; Jin, Mei Hua ; Han, Ying Hao ; Lee, Hwang ; Kim, Jin Man ; Kim, Sang Keun ; Yu, Dae Yeul ; Lee, Dong Seok ; Lee, Sang Ho. / Peroxiredoxin I is an indicator of microglia activation and protects against hydrogen peroxide-mediated microglial death. In: Biological and Pharmaceutical Bulletin. 2008 ; Vol. 31, No. 5. pp. 820-825.
@article{4337ade20efd48f296fe247bad30e90e,
title = "Peroxiredoxin I is an indicator of microglia activation and protects against hydrogen peroxide-mediated microglial death",
abstract = "Imbalance between oxidative stress and antioxidative defence system is generally known as one of mechanisms causing an oxidative stress-medieated neuropathogenesis. Peroxiredoxins (Prxs), a family of antioxidative enzymes neutralizing cellular hydroperoxides, was characterized recently, but their distributions and roles have not been resolved clearly or controversial in the central nervous system, Therefore, the present study was carried out to determine the specific cell types that express Prx I in the mouse brain and primary neural cells, and to examine its antioxidative role in the preferential cell types. Immunohistochemical reactivity for Prx I was detected dominantly in oligodendrocytes and rarely in microglia, whereas strong and specific immunoreactivity for Prx I was observed exclusively in microglia of primary neural cell culture. Further evidences for Prx I specificity were its relatively high expression in BV-2 microglial cells and its upregulated expression in microglia after lipopolysaccharide (LPS) stimulation. These results imply that Prx I can be used as an indicator of microglial activation. Inhibition of p38 MAPK ablated LPS-mediated Prx I upregulation and sensitized the microglia to H2O2-mediated cell death. These findings indicate that Prx I function as a scavenger for H2O2 generated during microglial activation. The results of this study will help in unraveling the neuropathologic roles of the six Prx isoforms in neural function.",
keywords = "Cell death, Hydrogen peroxide, Microglia, Peroxiredoxin I",
author = "Kim, {Sun Uk} and Hwang, {Chang Nam} and Sun, {Hu Nan} and Jin, {Mei Hua} and Han, {Ying Hao} and Hwang Lee and Kim, {Jin Man} and Kim, {Sang Keun} and Yu, {Dae Yeul} and Lee, {Dong Seok} and Lee, {Sang Ho}",
year = "2008",
month = "5",
day = "1",
doi = "10.1248/bpb.31.820",
language = "English",
volume = "31",
pages = "820--825",
journal = "Biological and Pharmaceutical Bulletin",
issn = "0918-6158",
publisher = "Pharmaceutical Society of Japan",
number = "5",

}

TY - JOUR

T1 - Peroxiredoxin I is an indicator of microglia activation and protects against hydrogen peroxide-mediated microglial death

AU - Kim, Sun Uk

AU - Hwang, Chang Nam

AU - Sun, Hu Nan

AU - Jin, Mei Hua

AU - Han, Ying Hao

AU - Lee, Hwang

AU - Kim, Jin Man

AU - Kim, Sang Keun

AU - Yu, Dae Yeul

AU - Lee, Dong Seok

AU - Lee, Sang Ho

PY - 2008/5/1

Y1 - 2008/5/1

N2 - Imbalance between oxidative stress and antioxidative defence system is generally known as one of mechanisms causing an oxidative stress-medieated neuropathogenesis. Peroxiredoxins (Prxs), a family of antioxidative enzymes neutralizing cellular hydroperoxides, was characterized recently, but their distributions and roles have not been resolved clearly or controversial in the central nervous system, Therefore, the present study was carried out to determine the specific cell types that express Prx I in the mouse brain and primary neural cells, and to examine its antioxidative role in the preferential cell types. Immunohistochemical reactivity for Prx I was detected dominantly in oligodendrocytes and rarely in microglia, whereas strong and specific immunoreactivity for Prx I was observed exclusively in microglia of primary neural cell culture. Further evidences for Prx I specificity were its relatively high expression in BV-2 microglial cells and its upregulated expression in microglia after lipopolysaccharide (LPS) stimulation. These results imply that Prx I can be used as an indicator of microglial activation. Inhibition of p38 MAPK ablated LPS-mediated Prx I upregulation and sensitized the microglia to H2O2-mediated cell death. These findings indicate that Prx I function as a scavenger for H2O2 generated during microglial activation. The results of this study will help in unraveling the neuropathologic roles of the six Prx isoforms in neural function.

AB - Imbalance between oxidative stress and antioxidative defence system is generally known as one of mechanisms causing an oxidative stress-medieated neuropathogenesis. Peroxiredoxins (Prxs), a family of antioxidative enzymes neutralizing cellular hydroperoxides, was characterized recently, but their distributions and roles have not been resolved clearly or controversial in the central nervous system, Therefore, the present study was carried out to determine the specific cell types that express Prx I in the mouse brain and primary neural cells, and to examine its antioxidative role in the preferential cell types. Immunohistochemical reactivity for Prx I was detected dominantly in oligodendrocytes and rarely in microglia, whereas strong and specific immunoreactivity for Prx I was observed exclusively in microglia of primary neural cell culture. Further evidences for Prx I specificity were its relatively high expression in BV-2 microglial cells and its upregulated expression in microglia after lipopolysaccharide (LPS) stimulation. These results imply that Prx I can be used as an indicator of microglial activation. Inhibition of p38 MAPK ablated LPS-mediated Prx I upregulation and sensitized the microglia to H2O2-mediated cell death. These findings indicate that Prx I function as a scavenger for H2O2 generated during microglial activation. The results of this study will help in unraveling the neuropathologic roles of the six Prx isoforms in neural function.

KW - Cell death

KW - Hydrogen peroxide

KW - Microglia

KW - Peroxiredoxin I

UR - http://www.scopus.com/inward/record.url?scp=44349135066&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44349135066&partnerID=8YFLogxK

U2 - 10.1248/bpb.31.820

DO - 10.1248/bpb.31.820

M3 - Article

C2 - 18451500

AN - SCOPUS:44349135066

VL - 31

SP - 820

EP - 825

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

SN - 0918-6158

IS - 5

ER -