Persistent infection of a gammaherpesvirus in the central nervous system

Hye Ri Kang, Hye Jeong Cho, Sungbum Kim, In Ho Song, Tae Sup Lee, Seungmin Hwang, Ren Sun, Moon Jung Song

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3 Citations (Scopus)

Abstract

Human gammaherpesvirus infections of the central nervous system (CNS) have been linked to various neurological diseases. Murine gammaherpesvirus 68 (MHV-68), genetically related and biologically similar to human gammaherpesviruses, infects the CNS in laboratory mice. However, viral persistency of MHV-68 has not been studied following CNS infection. In this study, we undertook the noninvasive bioluminescence imaging of a recombinant MHV-68 expressing the firefly luciferase (M3FL) to monitor virus progression after CNS infection. The M3FL virus inoculated in the brain systemically spread to the abdominal area in bioluminescence imaging, which was further confirmed by detection of viral genome and transcripts. The disseminated wild-type virus established latency in the spleen. Moreover, the treatment of the infected mice with CsA induced reactivation of latent MHV-68 from the brain and the spleen. Our results suggest that MHV-68 may persist both inside and outside the CNS once it gains access to the CNS.

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalVirology
Volume423
Issue number1
DOIs
Publication statusPublished - 2012 Feb 5

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Keywords

  • Bioluminescence imaging
  • Central nervous system
  • Gammaherpesvirus
  • Persistent infection

ASJC Scopus subject areas

  • Virology

Cite this

Kang, H. R., Cho, H. J., Kim, S., Song, I. H., Lee, T. S., Hwang, S., Sun, R., & Song, M. J. (2012). Persistent infection of a gammaherpesvirus in the central nervous system. Virology, 423(1), 23-29. https://doi.org/10.1016/j.virol.2011.11.012