Peyer's patch-mediated intestinal immune system modulating activity of pectic-type polysaccharide from peel of Citrus unshiu

Hyung Joo Suh, Hyun Seuk Yang, Kyung Soo Ra, Dong Ouk Noh, Ki Han Kwon, Jong Hyun Hwang, Kwang Won Yu

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

An intestinal immune system modulating polysaccharide (CUI-3IIb-3-2, 18 kDa) was purified from Citrus unshiu peel. CUI-3IIb-3-2 mainly comprised GalA, GlcA, Ara, Gal and Rha, and it consisted of 4-linked GalA, terminal Araf, 4- or 5-linked/3,4- or 3,5-branched Ara, terminal Gal, and 2-linked/2,4-branched Rha. After CUI-3IIb-3-2 digestion by endo-α-d-(1 → 4)-polygalacturonase, its hydrolysate was fractionated into PG-1 and PG-2. Methylation analyses of PG-1 and PG-2 using base-catalysed β-elimination suggested that CUI-3IIb-3-2 be assumed as pectic-type polysaccharide. Since the activities of PG-1 and PG-2 were potently decreased, the whole polysaccharide structure of CUI-3IIb-3-2 would be essential to maintain the activity. Meanwhile, when CUI-3IIb was orally administered in mice, bone marrow cell proliferation and GM-CSF/IL-6 production from Peyer's patch cell were significantly higher (1.76- and 2.03/2.51-fold, respectively) than a saline. Therefore, a pectic-type polysaccharide from citrus peel could stimulate Peyer's patches and produce hematopoietic growth factors resulted in bone marrow cell proliferation.

Original languageEnglish
Pages (from-to)1079-1086
Number of pages8
JournalFood Chemistry
Volume138
Issue number2-3
DOIs
Publication statusPublished - 2013 Jun 1

Keywords

  • Base-catalysed β-elimination
  • Citrus unshiu peel
  • Intestinal immune system modulating activity
  • Pectic-type polysaccharide
  • Peyer's patch

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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