Peyer's patch-mediated intestinal immune system modulating activity of pectic-type polysaccharide from peel of Citrus unshiu

Hyung Joo Suh, Hyun Seuk Yang, Kyung Soo Ra, Dong Ouk Noh, Ki Han Kwon, Jong Hyun Hwang, Kwang Won Yu

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

An intestinal immune system modulating polysaccharide (CUI-3IIb-3-2, 18 kDa) was purified from Citrus unshiu peel. CUI-3IIb-3-2 mainly comprised GalA, GlcA, Ara, Gal and Rha, and it consisted of 4-linked GalA, terminal Araf, 4- or 5-linked/3,4- or 3,5-branched Ara, terminal Gal, and 2-linked/2,4-branched Rha. After CUI-3IIb-3-2 digestion by endo-α-d-(1 → 4)-polygalacturonase, its hydrolysate was fractionated into PG-1 and PG-2. Methylation analyses of PG-1 and PG-2 using base-catalysed β-elimination suggested that CUI-3IIb-3-2 be assumed as pectic-type polysaccharide. Since the activities of PG-1 and PG-2 were potently decreased, the whole polysaccharide structure of CUI-3IIb-3-2 would be essential to maintain the activity. Meanwhile, when CUI-3IIb was orally administered in mice, bone marrow cell proliferation and GM-CSF/IL-6 production from Peyer's patch cell were significantly higher (1.76- and 2.03/2.51-fold, respectively) than a saline. Therefore, a pectic-type polysaccharide from citrus peel could stimulate Peyer's patches and produce hematopoietic growth factors resulted in bone marrow cell proliferation.

Original languageEnglish
Pages (from-to)1079-1086
Number of pages8
JournalFood Chemistry
Volume138
Issue number2-3
DOIs
Publication statusPublished - 2013 Jun 1

Fingerprint

Peyer's Patches
Citrus unshiu
Peyer's patches
Citrus
Immune system
Polysaccharides
immune system
Immune System
polysaccharides
bone marrow cells
Cell proliferation
Bone Marrow Cells
cell proliferation
Bone
Cell Proliferation
citrus peels
Polygalacturonase
Methylation
Granulocyte-Macrophage Colony-Stimulating Factor
polygalacturonase

Keywords

  • Base-catalysed β-elimination
  • Citrus unshiu peel
  • Intestinal immune system modulating activity
  • Pectic-type polysaccharide
  • Peyer's patch

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Peyer's patch-mediated intestinal immune system modulating activity of pectic-type polysaccharide from peel of Citrus unshiu. / Suh, Hyung Joo; Yang, Hyun Seuk; Ra, Kyung Soo; Noh, Dong Ouk; Kwon, Ki Han; Hwang, Jong Hyun; Yu, Kwang Won.

In: Food Chemistry, Vol. 138, No. 2-3, 01.06.2013, p. 1079-1086.

Research output: Contribution to journalArticle

Suh, Hyung Joo ; Yang, Hyun Seuk ; Ra, Kyung Soo ; Noh, Dong Ouk ; Kwon, Ki Han ; Hwang, Jong Hyun ; Yu, Kwang Won. / Peyer's patch-mediated intestinal immune system modulating activity of pectic-type polysaccharide from peel of Citrus unshiu. In: Food Chemistry. 2013 ; Vol. 138, No. 2-3. pp. 1079-1086.
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abstract = "An intestinal immune system modulating polysaccharide (CUI-3IIb-3-2, 18 kDa) was purified from Citrus unshiu peel. CUI-3IIb-3-2 mainly comprised GalA, GlcA, Ara, Gal and Rha, and it consisted of 4-linked GalA, terminal Araf, 4- or 5-linked/3,4- or 3,5-branched Ara, terminal Gal, and 2-linked/2,4-branched Rha. After CUI-3IIb-3-2 digestion by endo-α-d-(1 → 4)-polygalacturonase, its hydrolysate was fractionated into PG-1 and PG-2. Methylation analyses of PG-1 and PG-2 using base-catalysed β-elimination suggested that CUI-3IIb-3-2 be assumed as pectic-type polysaccharide. Since the activities of PG-1 and PG-2 were potently decreased, the whole polysaccharide structure of CUI-3IIb-3-2 would be essential to maintain the activity. Meanwhile, when CUI-3IIb was orally administered in mice, bone marrow cell proliferation and GM-CSF/IL-6 production from Peyer's patch cell were significantly higher (1.76- and 2.03/2.51-fold, respectively) than a saline. Therefore, a pectic-type polysaccharide from citrus peel could stimulate Peyer's patches and produce hematopoietic growth factors resulted in bone marrow cell proliferation.",
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AU - Yu, Kwang Won

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AB - An intestinal immune system modulating polysaccharide (CUI-3IIb-3-2, 18 kDa) was purified from Citrus unshiu peel. CUI-3IIb-3-2 mainly comprised GalA, GlcA, Ara, Gal and Rha, and it consisted of 4-linked GalA, terminal Araf, 4- or 5-linked/3,4- or 3,5-branched Ara, terminal Gal, and 2-linked/2,4-branched Rha. After CUI-3IIb-3-2 digestion by endo-α-d-(1 → 4)-polygalacturonase, its hydrolysate was fractionated into PG-1 and PG-2. Methylation analyses of PG-1 and PG-2 using base-catalysed β-elimination suggested that CUI-3IIb-3-2 be assumed as pectic-type polysaccharide. Since the activities of PG-1 and PG-2 were potently decreased, the whole polysaccharide structure of CUI-3IIb-3-2 would be essential to maintain the activity. Meanwhile, when CUI-3IIb was orally administered in mice, bone marrow cell proliferation and GM-CSF/IL-6 production from Peyer's patch cell were significantly higher (1.76- and 2.03/2.51-fold, respectively) than a saline. Therefore, a pectic-type polysaccharide from citrus peel could stimulate Peyer's patches and produce hematopoietic growth factors resulted in bone marrow cell proliferation.

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