PGK1 induction by a hydrogen peroxide treatment is suppressed by antioxidants in human colon carcinoma cells

Chan Ho Jang, In Ae Lee, Young Ran Ha, Jinkyu Lim, Mi Kyung Sung, Sung Joon Lee, Jong Sang Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Few protein biomarkers for oxidative stress have been reported. In this study, we attempted to identify the proteins selectively overexpressed in human colon tumor cells by treating with hydrogen peroxide as oxidative stress. A proteomic analysis followed by western blotting showed that phosphoglycerate kinase 1 (PGK1) was induced by hydrogen peroxide in a dose-dependent manner, while its expression was suppressed by a co-treatment with delphinidin, a known antioxidant. Furthermore, several antioxidants, including α-tocopherol, butylated hydroxytoluene (BHT), and Trolox, also inhibited the PGK1 induction caused by hydrogen peroxide. The data suggest that PGK1 might be a potential protein biomarker of intracellular oxidative status.

Original languageEnglish
Pages (from-to)1799-1808
Number of pages10
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number7
DOIs
Publication statusPublished - 2008

Keywords

  • Antioxidant
  • Biomarker
  • Oxidative stress
  • Phosphoglycerate kinase 1 (PGK1)

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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