PH-responsive water soluble smart vesicles containing a bis(styryl)benzene derivative for two-photon microscopy imaging

Okhil Kumar Nag, Chang Su Lim, Bao Lam Nguyen, Boram Kim, Jihye Jang, Ji Hee Han, Bong-Rae Cho, Han Young Woo

Research output: Contribution to journalArticle

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Abstract

We report self-assembled polymeric nanovesicles composed of 1,4-bis{4′-[N,N-bis(6′′-trimethylammoniumhexyl)amino]styryl} benzene tetrabromide (C1), poly[(ethylene oxide)-block-(sodium 2-acrylamido-2-methyl-1-propane sulfonate)] (E m-A n), and hexadecyltrimethylammonium bromide (C 16). Transmission electron microscopy (TEM) micrographs confirm the vesicular bilayer structures and atomic force microscopy (AFM) images show that the C1/E m-A n/C 16 complexes form spherical nanostructures with a particle size ranging from 40 to 80 nm. The encapsulation of C1 inside the nanovesicles enhances ∼2-fold the fluorescence quantum yield (η) and two-photon action cross-section (ηδ, where δ is the two-photon absorption cross-section), and allows internalization into the cells, as revealed by the bright two-photon microscopy (TPM) images of human cervical epithelioid carcinoma (HeLa) cells labeled with the nanovesicles. Moreover, nanovesicles containing a chemotherapeutic drug and a neutral molecule can also be prepared. Furthermore, the C1/vesicular complex is disassembled under acidic conditions, highlighting its potential as a pH-responsive smart nanocarrier for the intracellular drug delivery. These results suggest a new possibility of using nanovesicles as efficient two-photon probes for TPM imaging and possibly as nanocarriers for intracellular drug delivery.

Original languageEnglish
Pages (from-to)1977-1984
Number of pages8
JournalJournal of Materials Chemistry
Volume22
Issue number5
DOIs
Publication statusPublished - 2012 Feb 7

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Benzene Derivatives
Benzene
Microscopic examination
Photons
Derivatives
Imaging techniques
Water
Drug delivery
Propane
Quantum yield
Polyethylene oxides
Encapsulation
Atomic force microscopy
Nanostructures
Sodium
Fluorescence
Particle size
Transmission electron microscopy
Molecules
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Materials Chemistry
  • Chemistry(all)

Cite this

PH-responsive water soluble smart vesicles containing a bis(styryl)benzene derivative for two-photon microscopy imaging. / Nag, Okhil Kumar; Lim, Chang Su; Nguyen, Bao Lam; Kim, Boram; Jang, Jihye; Han, Ji Hee; Cho, Bong-Rae; Woo, Han Young.

In: Journal of Materials Chemistry, Vol. 22, No. 5, 07.02.2012, p. 1977-1984.

Research output: Contribution to journalArticle

Nag, Okhil Kumar ; Lim, Chang Su ; Nguyen, Bao Lam ; Kim, Boram ; Jang, Jihye ; Han, Ji Hee ; Cho, Bong-Rae ; Woo, Han Young. / PH-responsive water soluble smart vesicles containing a bis(styryl)benzene derivative for two-photon microscopy imaging. In: Journal of Materials Chemistry. 2012 ; Vol. 22, No. 5. pp. 1977-1984.
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